LIN28 antibody

Principal name

LIN28 antibody

Alternative names for LIN28 antibody

LIN-28, LIN28A, CSDD1, ZCCHC1, Lin-28 homolog A

SwissProt ID

Q45KJ5 (Chick), Q5EB47 (Xentr), Q8JHC4 (Xenla), Q8K3Y3 (Mouse), Q9H9Z2 (Human)

Gene ID

79727 (LIN28A), 4779727 (A2C4J7)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Gt (Goat), Rb (Rabbit), Sh (Sheep)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Dot blot (Dot), Flow Cytometry (F)

Background of LIN28 antibody

Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.

General readings

Peng, S., et al. Oncogene 29(14):2153-2159(2010)
Qiu, C., et al. Nucleic Acids Res. 38(4):1240-1248(2010)
Iliopoulos, D., et al. Cell 139(4):693-706(2009)
Heo, I., et al. Cell 138(4):696-708(2009)
West, J.A., et al. Nature 460(7257):909-913(2009)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

LIN28 (Center) antibody

LIN28A Antibody (Center) (Cat. #TA324388) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the LIN28A antibody detected the LIN28A protein (arrow). Rabbit Ig Purified Hu, Ms F, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

LIN28 antibody

Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only. Rabbit IgG Purified Hu Dot, E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

LIN28 antibody

Western blot analysis of Lin28 in Raji cell lysate with Lin28 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

LIN28 antibody

Predicted band size: 23 kDa . Positive control: HepG2 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lysate: 30 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

LIN28 antibody

Western blot analysis of Lin28 in Raji cell lysate with Lin28 antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide. Mouse IgG2b 1G9H9 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

LIN28 antibody

WB Suggested Anti-LIN28 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Hela cell lysate Rabbit IgG Purified Bov, Can, Gt, Hu, Por, Rb, Rt, Sh P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

LIN28 antibody

Western Blot: Lin28 Antibody [TA336788] - Analysis of LIN28 in NTERA-2 cell lysate Rabbit Purified Hu, Ms C, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

LIN28 antibody

Western blot detection of LIN28 in NTERA and F9 cell lysates using LIN28A mouse mAb (1:1000 diluted).Predicted band size:26KDa.Observed band size:26KDa. Mouse IgG2a 2C1-F9-A2 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

LIN28 antibody

Confocal immunofluorescence analysis of NTERA-2 cells using LIN28 antibody Cat.-No AM06277PU-N (green). Blue: DRAQ5 fluorescent DNA dye. Mouse IgG1 6D1F9 Aff - Purified Hu E, ICC/IF, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

LIN28 antibody

Figure 2. Formalin-fixed and paraffin-embedded human testis tissue reacted with LIN28A Monoclonal Antibody (Cat.#AM11048PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated. Mouse IgG1 55CT58.12.1 Purified Hu P, WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

LIN28 (C-term) antibody

Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

LIN28 (1-5) antibody

Western blot analysis of extracts from HepG2 cells using Lin28 Antibody AP26066PU-N. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €270.00
  Acris Antibodies GmbH

LIN28 (1-5) antibody

Western blot analysis of extracts from HepG2 cells using Lin28 Antibody AP26066PU-N. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µl / €230.00
  Acris Antibodies GmbH

LIN28 Control Peptide

LIN28
50 µg / €160.00
  Acris Antibodies GmbH

LIN28 antibody

Western blot analysis of LIN28 Antibody Cat.-No AP52486PU-N in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the LIN28 antibody detected the LIN28 protein (arrow). Rabbit Ig Purified Hu, Ms F, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

LIN28 antibody

Immunofluorescence of purified MaxPab antibody to LIN28 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

LIN28 antibody

LIN28 Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

LIN28 antibody

LIN28 Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

LIN28 antibody

Human Testis; Immunohistochemistry with Human Testis lysate tissue at an antibody concentration of 5.0ug/ml using anti-LIN28 antibody (ARP34477_P050); LIN28 antibody - N-terminal region (ARP34477_P050) in Human Testis cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

LIN28 antibody

ELISA: LIN28 Antibody [32140002] Rabbit Hu, Ms Dot, E, P
50 µg / €500.00
  Novus Biologicals Inc.

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