LIPA antibody
Principal name
LIPA antibody
Alternative names for LIPA antibody
LIPA, Lysosomal acid lipase/cholesteryl ester hydrolase, LAL, Acid cholesteryl ester hydrolase, EC=3.1.1.13, Sterol esterase, Lipase A, Cholesteryl esterase
Gene ID
3988 (LIPA)
Ncbi ID
9606, NP_000226, NP_000226.2, NM_000235, AAH12287, NM_001127605
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of LIPA antibody
Lysosomal acid lipase (LAL), with 378-amino acid protein( 43-54 kDa), functions in the lysosome to catalyze the hydrolysis of cholesteryl esters and triglycerides which are taken up by receptor-mediated endocytosis. An inherited deficiency or low activity of human lysosomal acid lipase results in the intralysosomal storage of the respective lipid substrates. So it is also responsible for the rare conditions of Wolman disease and cholesteryl ester storage disease (CESD). As the enzyme is synthesized by all nucleated cells, lipid-laden cells are found in all organs, particularly in liver, spleen, the adrenal and the hemopoietic system, and in the intestine as well as in the lymph nodes, lungs, testes, and ovaries.
Western Blot analysis of LIPA expression in transfected 293T cell line by LIPA monoclonal antibody (M01), clone 1F9.Lane 1: LIPA transfected lysate(45 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged LIPA is approximately 10ng/ml as a capture antibody.
Western Blot: LAL Antibody (9G7F12) [NB110-60489] - Figure 1: Western blot analysis using anti-human LAL monoclonal antibody against truncated LAL recombinant protein.
LIPA MaxPab polyclonal antibody. Western Blot analysis of LIPA expression in human pancreas.
Western Blot analysis of LIPA expression in transfected 293T cell line (H00003988-T01) by LIPA MaxPab polyclonal antibody.Lane 1: LIPA transfected lysate(43.89 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of LIPA expression in transfected 293T cell line (H00003988-T01) by LIPA MaxPab polyclonal antibody.Lane 1: LIPA transfected lysate(45.40 KDa).Lane 2: Non-transfected lysate.
LIPA MaxPab rabbit polyclonal antibody. Western Blot analysis of LIPA expression in K-562.
Western Blot: Lysosomal acid lipase Lysate [NBL1-12546] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for LIPA
Western blot analysis using LIPA monoclonal antibody, clone 9G7F12 ( Cat # MAB5527 ) against truncated LIPA recombinant protein.
Immunohistochemical of paraffin-embedded human colon cancer using 12956-1-AP(LIPA antibody) at dilution of 1:100 (under 10x lens)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12956-1-AP(LIPA antibody) at dilution of 1:500
HeLa cells were subjected to SDS PAGE followed by western blot with 12956-1-AP(LIPA antibody) at dilution of 1:500
Y79 cells were subjected to SDS PAGE followed by western blot with 12956-1-AP(LIPA antibody) at dilution of 1:400
HepG2 cells were subjected to SDS PAGE followed by western blot with 12956-1-AP(LIPA antibody) at dilution of 1:600
HepG2 cells were subjected to SDS PAGE followed by western blot with 12956-1-AP(LIPA antibody) at dilution of 1:1000
HT-1080 cells were subjected to SDS PAGE followed by western blot with 12956-1-AP(LIPA antibody) at dilution of 1:500
HeLa cells were subjected to SDS PAGE followed by western blot with 12956-1-AP(LIPA antibody) at dilution of 1:600
Sample (50 ug of whole cell lysate) A: mouse Liver 10% SDS PAGE GTX101169 diluted at 1:1000
Sample (30 ug of whole cell lysate) A: A549 B: H1299 10% SDS PAGE GTX101169 diluted at 1:1000
Hum. Fetal Heart; Host:Rabbit. Target Name:LIPA. Sample Tissue:Human Fetal Heart. Antibody Dilution: 1.0ug/ml.; LIPA antibody - N-terminal region (ARP61109_P050) in Hum. Fetal Heart cells using Western Blot
Human HepG2; WB Suggested Anti-LIPA Antibody. . Titration: 1.0 ug/ml. . Positive Control: HepG2 Whole Cell. LIPA is supported by BioGPS gene expression data to be expressed in HepG2.; LIPA antibody - N-terminal region (ARP61109_P050) in Human HepG2 cells using Western Blot
Immunocytochemistry: Lysosomal acid lipase Antibody [NBP1-54155] - ICC staining of LIPA in HepG2 cells with FITC (green). Nuclei were counterstained with Dapi (blue).
Western Blot: Lysosomal acid lipase Antibody [NBP1-54155] - WB detection of LIPA in HepG2 whole cell extract.
Immunohistochemistry: Lysosomal acid lipase Antibody [NBP1-54155] - IHC staining of LIPA in mouse stomach smooth muscle.
Western blot analysis using LAL mouse mAb against LAL recombinant protein.
Western blot analysis using anti-LAL monoclonal antiobdy against LAL recombinant protein.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA309730 | LIPA antibody |
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| Rabbit Polyclonal Lipase A Antibody | |||||||
 |
Rabbit | Purified | Hu, Ms | C, ICC/IF, WB |
0.1 ml /
€325.00
|
||
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
| TA343110 | LIPA antibody |
||||||
| Rabbit Polyclonal Anti-LIPA Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Eq, Hu, Por, Rb, Rt, Sh | WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| AM06118PU-N | LIPA antibody |
||||||
 |
Mouse | IgG2a | 9G7F12 | Purified | Hu | E, WB |
0.1 ml /
€360.00
|
| Acris Antibodies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301637 | LIPA (transcript variant 2) |
||||
| Recombinant protein of human lipase A, lysosomal acid, cholesterol esterase (LIPA), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€680.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY426820 | LIPA overexpression lysate |
|
 |
0.1 mg /
€280.00
|
|
| OriGene Technologies, Inc. |
