LIPE antibody

Principal name

LIPE antibody

Alternative names for LIPE antibody

LIPE, Hormone-sensitive lipase, HSL, EC=3.1.1.79

SwissProt ID

P15304 (Rat), P16386 (Bovin), P54310 (Mouse), Q05469 (Human), Q68J42 (Pig), Q9R101 (Spetr)

Gene ID

3991 (LIPE), 25330 (Lipe), 16890, 9388 (LIPG)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Por (Porcine)

Available hosts

Chicken, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Frozen Sections (C), Immunoprecipitation (IP)

Background of LIPE antibody

HSL (hormone-sensitive lipase) is a key enzyme in lipid metabolism regulation in several tissues and this intracellular neutral lipase hydrolyzes triacylglycerols (TG), diacylglycerols (DG), and monoacylglycerols, as well as cholesterol and retinyl esters. While ATGL selectively performs the first step in TG hydrolysis, HSL is the most active enzyme against DG, which gets hydrolyzed faster than TG. HSL has been shown to express in several non-adipose tissues, including ovary, adrel gland, heart, skeletal and smooth muscle, placenta, and pancreatic beta-cells, as well as testes. Under basal conditions, HSL is exclusively found in the cytosol of adipocytes, whereas upon phosphorylation mediated activation via PKA, HSL translocates from cytosol to lipid droplet surfaces for participation in lipolysis. HSL activity is regulated primarily by serine phosphorylation, which is stimulatory at Ser-563/Ser-659 via PKA and inhibitory at Ser-565 via AMPK. Mice lacking HSL have decreased nonesterified fatty acid (NEFA) and increased cholesterol concentrations in plasma and are resistant to diet-induced obesity. HSL has emerged as a potential target for lipid disorders and obesity.

General readings

Bezaire, V., et al. FEBS Lett. 583(18):3045-3049(2009)
Chen, H.H., et al. Am. J. Clin. Nutr. 90(2):255-262(2009)
Kuzmin, A., et al. Biol. Reprod. 81(2):319-326(2009)
Bezaire, V., et al. J. Biol. Chem. 284(27):18282-18291(2009)
Drenos, F., et al. Hum. Mol. Genet. 18(12):2305-2316(2009)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

LIPE antibody

Western blot analysis of lipe in human lymph node tissue lysate with lipe antibody at (A) 0.5 and (B) 1 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

LIPE antibody

Western blot analysis of extracts from HeLa cells, treated with Calyculin A (100nM, 30mins), using HSL (Ab-554) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

LIPE antibody

Predicted cell location: Cell membrane, Cytoplasm . Positive control: Human esophagus cancer tissue. Recommended dilution: 1/10-50  The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using LIPE antibody at dilution 1/10, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

LIPE antibody

Western blot analysis of HSL expression in CalyculinA treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

LIPE antibody

Western blot analysis of HSL expression in Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

LIPE antibody

Western Blot: HSL Antibody [TA336511] - Detection of HSL using TA336511. Lane 1: Liver extract (negative control) Lane 2: Fat extract (10ug) (positive sample). Rabbit Purified Hu, Ms, Rt WB
50 µl / €325.00
  OriGene Technologies, Inc.

LIPE antibody

Immunohistochemistry (IHC) analyzes of HSL antibody (Cat.-No.: AP01276PU-N) in paraffin-embedded human tonsil tissue. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

LIPE antibody

AP07322PU-N LIPE antibody staining of Formalin-Fixed Paraffin-Embedded Human Testis at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

LIPE Control Peptide

LIPE
50 µg / €160.00
  Acris Antibodies GmbH

LIPE (C-term) antibody

Western blot analysis of LIPE Antibody (C-term) Cat.-No AP52494PU-N in HL-60 cell line lysates (35ug/lane). LIPE (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

LIPE antibody

LIPE Rabbit Serum Ms C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

LIPE antibody

Western blot analysis of Lipe using Lipe polyclonal antibody ( Cat # PAB12105 ). Lane 1 : Liver extract ( negative control ). Lane 2 : Fat extract ( 10µg ) ( positive sample ) . Rabbit Hu, Rt WB
50 µl / €470.00
  Abnova Taiwan Corp.

LIPE antibody

Immunohistochemistry of LIPE in human lymph node tissue with LIPE polyclonal antibody ( Cat # PAB12975 ) at 2.5 µg/ml. Rabbit Hu, Ms, Rt P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

LIPE pSer855/554 antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using HSL (Phospho-Ser855/554) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

LIPE pSer855/554 antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using HSL (Phospho-Ser855/554) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

LIPE pSer552 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HSL (Phospho-Ser552) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

LIPE pSer552 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HSL (Phospho-Ser552) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

LIPE antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using HSL (Ab-554) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

LIPE antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using HSL (Ab-554) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

LIPE antibody

Immunohistochemistry analysis of paraffin-embedded human tonsil tissue, using HSL (Ab-552) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

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