Lipoxygenase 1 antibody

Principal name

Lipoxygenase 1 antibody

Alternative names for Lipoxygenase 1 antibody

LOX1, Lipoxygenase-1, EC 1.13.11.12

SwissProt ID

P08170 (Soybn), P29114 (Horvu), P37831 (Soltu), P38414 (Lencu), Q06327 (Arath), Q76I22 (Orysj)

Gene ID

841944 (LOX1)

Available reactivities

Arabidopsis

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Lipoxygenase 1 antibody

Samples of Arabidopsis thaliana (2), Olea europaea (3), Lilium Longiflorum (4), Lupinus luteus (5) were ground in liquid nitrogen to a very fine powder using a mortar and pestle and resuspended in 1.5 ml of extraction buffer (4% SDS, 2% 2-mercaptoethanol, 2 mM PMSF, 100 mM Tris-HCl pH 8.5). The samples were incubated for 3 min at 80°C. Protein suspensions were clarified by centrifugation at 13,500 g for 10 min at room temperature and the resulting supernatants were used. Total proteins (25 µg per sample) were separated by SDS-PAGE on CriterionTMTGXTM Precast Gel (Bio-Rad, USA) using CriterionTM Cell apparatus (Bio-Rad). Proteins were electroblotted onto a PVDF membrane using Trans-Blot® TurboTM Transfer Pack (Bio-Rad) in a Trans-Blot® TurboTM Transfer System (Bio-Rad). The membrane was blocked for 1 h in solution containing 1 % (w/v) non-fat dry milk in TRIS-buffered saline (TBS) buffer, pH 7.4. The membrane was incubated in the primary antibody at a dilution of 1: 1000 in TBS buffer containing 1 % (w/v) non-fat dry milk over night at 4°C with agitation. A DyLight 488 conjugated anti-rabbit IgG (AS10 831, Agrisera), diluted 1:2000 in TBS buffer for 2 h, served as the secondary antibody. The signal was detected in a Pharos FX molecular imager (Bio-Rad). Line 1 contains LOX protein from Sigma. Courtesy of Dr. Agnieszka Zienkiewicz, CSIC, Spain Rabbit Serum Arabidopsis, Glycine max, Lilium longiflorum, Lupinus luteus, Olea europaea. WB
50 µl / €300.00
  AgriSera AB

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