LOXL1 antibody

Principal name

LOXL1 antibody

Alternative names for LOXL1 antibody

LOXL, Lysyl oxidase homolog 1, Lysyl oxidase-like protein 1, LOL

SwissProt ID

P97873 (Mouse), Q08397 (Human), Q95L39 (Bovin)

Gene ID

4016 (LOXL1), 2540163 (aap1)

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Dot blot (Dot), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP)

Background of LOXL1 antibody

Elastic fibers provide tissues with elasticity which is critical to the function of arteries, lungs, skin, and other dynamic organs. Loss of elasticity is a major contributing factor in aging and diseases. However, the mechanism of elastic fiber development and assembly is poorly understood. Lysyl oxidases are extracellular copper-dependent enzymes that catalyzing the formation of lysine and hydroxylysine-derived cross-links in collagens and lysine-derived cross-links in elastin. These cross-links are essential for the tensile strength of collagens and the rubber-like properties of elastin, both abundant extracellular matrix proteins that are necessary for the structural integrity and function of connective tissues. Lysyl oxidases (LOX) are involved in cross linking of elastin and collagens and the LOX family comprise of LOX and 4 LOX-like enzymes, all are likely to catalyze cross-linked formation in collagens and elastin. The specific functions associated with these isoforms are not completely understood at present. The LOX are essential and crucial for development of the cardiovascular system and LOX null mice died prenatally of aortic aneurysms and cardiovascular dysfunction (1). LOX family members are also essential for respiratory system and the integrity of elastic and collagen fibers in the lungs and skin.
Lysyl oxidase catalyzes the reaction that generates peptidyl-δ -hydroxy-α -aminoadipic-δ -semialdehyde and peptidyl-α -aminoadipic-δ -semialdehyde from hydroxylysine and lysine residues which is the final enzymatic reaction required for collagen cross linking. The newly formed aldehyldes fromed by these enzymatic reaction then spontaneously react with other aldehydes, unmodified lysine and hydroxylysine to form a variety fo intra and inter molecular cross links that are found in collagens and elastins (2). Lysyl oxidase is a copper-dependent amine oxidase. It is secreted from fibrogenic cells as a pro-enzyme that appears to have little or no enzyme activity, and is processed in the extracellular environment to produce the active 32 kDa enzyme and an 18 kDa propeptide (3). LOXL1 is a 608aa (605in mouse and 400 in human) protein. Antibodies raised against LOXL1 strongly reacted with a 90kDa protein and a 34 kDa smaller subunit essentially the active LOX subunit in PC-LOXL1 samples. The 400 amino acid sequence in human is processed by cleavage of 207 amino acids form the N-terminal.

General readings

Ozaki,M., (er) Invest. Ophthalmol. Vis. Sci. (2008) In press

17 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

LOXL1 antibody

Western blot analysis of skin lysates with anti-LOXL1 antibody (1:200). Rabbit Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

LOXL1 antibody

Western blot analysis of skin lysates with anti-LOXL1 antibody (1:500). Rabbit Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

LOXL1 antibody

WB Suggested Anti-LOXL1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

LOXL1 antibody

WB Suggested Anti-LOXL1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Lung Rabbit IgG Purified Bov, Eq, GP, Hu, Ms, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

LOXL1 Control Peptide

LOXL1
0.25 mg / €250.00
  Acris Antibodies GmbH

LOXL1 (570-590) antibody

Western blot with AP10123PU-N  at 1:500. Rabbit Aff - Purified Hu Dot, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

LOXL1 antibody

Western Blot analysis of LOXL1 expression in transfected 293T cell line (H00004016-T01) by LOXL1 MaxPab polyclonal antibody.

Lane 1: LOXL1 transfected lysate(63.14 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

LOXL1 antibody

LOXL1 Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

LOXL1 antibody

Western Blot analysis of LOXL1 expression in transfected 293T cell line (H00004016-T01) by LOXL1 MaxPab polyclonal antibody.

Lane 1: LOXL1 transfected lysate(63.10 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

LOXL1 antibody

Western blot of Loxl1 polyclonal antibody ( Cat # PAB8967 ) with protein-coupled Loxl1. Antibody dilution at 1 : 500. Rabbit Hu, Rt Dot, E, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

LOXL1 antibody

Human HepG2; WB Suggested Anti-LOXL1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; LOXL1 antibody - N-terminal region (ARP54780_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

LOXL1 antibody

Human Lung; WB Suggested Anti-LOXL1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Lung; LOXL1 antibody - middle region (ARP54781_P050) in Human Lung cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

LOXL1 antibody

Western Blot: LOXL1 Antibody [NBP1-57939] - This Anti-LOXL1 antibody was used in Western Blot of Fetal Lung tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

LOXL1 antibody

Immunohistochemistry-Paraffin: LOXL1 Antibody [NBP1-82827] - Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Antibody Pairs

Catalog No.    

LOXL1 (Human) IP-WB Antibody Pair

LOXL1 (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

LOXL1 293T Cell Transient Overexpression Lysate(Denatured)

LOXL1 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

LOXL1 overexpression lysate

LOXL1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

17 Item(s)

per page
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