LOXL1 antibody

Principal name

LOXL1 antibody

Alternative names for LOXL1 antibody

LOXL, Lysyl oxidase homolog 1, Lysyl oxidase-like protein 1, LOL

SwissProt ID

P97873 (Mouse), Q08397 (Human), Q95L39 (Bovin)

Gene ID

4016 (LOXL1), 16949 (Loxl1)

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Dot blot (Dot), Enzyme Immunoassay (E), Immunoprecipitation (IP)

Background of LOXL1 antibody

Elastic fibers provide tissues with elasticity which is critical to the function of arteries, lungs, skin, and other dynamic organs. Loss of elasticity is a major contributing factor in aging and diseases. However, the mechanism of elastic fiber development and assembly is poorly understood. Lysyl oxidases are extracellular copper-dependent enzymes that catalyzing the formation of lysine and hydroxylysine-derived cross-links in collagens and lysine-derived cross-links in elastin. These cross-links are essential for the tensile strength of collagens and the rubber-like properties of elastin, both abundant extracellular matrix proteins that are necessary for the structural integrity and function of connective tissues. Lysyl oxidases (LOX) are involved in cross linking of elastin and collagens and the LOX family comprise of LOX and 4 LOX-like enzymes, all are likely to catalyze cross-linked formation in collagens and elastin. The specific functions associated with these isoforms are not completely understood at present. The LOX are essential and crucial for development of the cardiovascular system and LOX null mice died prenatally of aortic aneurysms and cardiovascular dysfunction (1). LOX family members are also essential for respiratory system and the integrity of elastic and collagen fibers in the lungs and skin.
Lysyl oxidase catalyzes the reaction that generates peptidyl-δ -hydroxy-α -aminoadipic-δ -semialdehyde and peptidyl-α -aminoadipic-δ -semialdehyde from hydroxylysine and lysine residues which is the final enzymatic reaction required for collagen cross linking. The newly formed aldehyldes fromed by these enzymatic reaction then spontaneously react with other aldehydes, unmodified lysine and hydroxylysine to form a variety fo intra and inter molecular cross links that are found in collagens and elastins (2). Lysyl oxidase is a copper-dependent amine oxidase. It is secreted from fibrogenic cells as a pro-enzyme that appears to have little or no enzyme activity, and is processed in the extracellular environment to produce the active 32 kDa enzyme and an 18 kDa propeptide (3). LOXL1 is a 608aa (605in mouse and 400 in human) protein. Antibodies raised against LOXL1 strongly reacted with a 90kDa protein and a 34 kDa smaller subunit essentially the active LOX subunit in PC-LOXL1 samples. The 400 amino acid sequence in human is processed by cleavage of 207 amino acids form the N-terminal.

General readings

Ozaki,M., (er) Invest. Ophthalmol. Vis. Sci. (2008) In press

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

LOXL1 antibody

  Rabbit anti-LOXL1 polyclonal antibody  
Western blot analysis of skin lysates with anti-LOXL1 antibody (1:200). Rabbit Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

LOXL1 antibody

  Rabbit anti-LOXL1 polyclonal antibody  
Western blot analysis of skin lysates with anti-LOXL1 antibody (1:500). Rabbit Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

LOXL1 antibody

  Rabbit Polyclonal Anti-LOXL1 Antibody  
WB Suggested Anti-LOXL1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

LOXL1 antibody

  Rabbit Polyclonal Anti-LOXL1 Antibody  
WB Suggested Anti-LOXL1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Lung Rabbit IgG Purified Bov, Eq, GP, Hu, Ms, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

LOXL1 antibody

  Rabbit Polyclonal Anti-LOXL1 Antibody  
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: Mouse skin tissue, Primary antibody: (LOXL1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

LOXL1 - human gene knockout kit via CRISPR

LOXL1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

LOXL1 - human gene knockout kit via CRISPR

LOXL1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

LOXL1 - human gene knockout kit via CRISPR

LOXL1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

LOXL1 - human gene knockout kit via CRISPR

LOXL1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Loxl1 - mouse gene knockout kit via CRISPR

Loxl1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Loxl1 - mouse gene knockout kit via CRISPR

Loxl1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

LOXL1 overexpression lysate

LOXL1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, LOXL1 (mGFP-tagged) - Human lysyl oxidase-like 1 (LOXL1), 200 uL, >10^7 TU/mL

Lenti ORF particles, LOXL1 (mGFP-tagged) - Human lysyl oxidase-like 1 (LOXL1), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, LOXL1 (Myc-DDK tagged) - Human lysyl oxidase-like 1 (LOXL1) , 200 uL, >10^7 TU/mL

Lenti ORF particles, LOXL1 (Myc-DDK tagged) - Human lysyl oxidase-like 1 (LOXL1) , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Loxl1 (GFP-tagged) - Mouse lysyl oxidase-like 1 (Loxl1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Loxl1 (GFP-tagged) - Mouse lysyl oxidase-like 1 (Loxl1), 200 uL, >10^7 TU/mL  
200 µl / €1,260.00
  OriGene Technologies, Inc.

Lenti ORF particles, Loxl1 (Myc-DDK-tagged) - Mouse lysyl oxidase-like 1 (Loxl1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Loxl1 (Myc-DDK-tagged) - Mouse lysyl oxidase-like 1 (Loxl1), 200 uL, >10^7 TU/mL  
200 µl / €1,130.00
  OriGene Technologies, Inc.

16 Item(s)

per page
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