LRP12 / ST7 antibody
Principal name
LRP12 / ST7 antibody
Alternative names for LRP12 / ST7 antibody
Low-density lipoprotein receptor-related protein 12
Ncbi ID
-, NP_001129175.1, NP_038465, NP_001129175, NP_766402.1, BC058345, NM_172814, NM_013437, NM_001135703
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)
Background of LRP12 / ST7 antibody
- Kit Component:
- - KN309427G1, Lrp12 gRNA vector 1 in pCas-Guide vector
- KN309427G2, Lrp12 gRNA vector 2 in pCas-Guide vector
- KN309427D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Garnis, C., et al. Oncogene 23(14):2582-2586(2004)
Battle, M.A., et al. Biochemistry 42(24):7270-7282(2003)
Qing, J., et al. Oncogene 18(2):335-342(1999)
Western blot analysis of LRP12 expression in Jurkat (A); HeLa (B); SP2/0 (C) whole cell lysates.
Sample(30 ug whole cell lysate). A:Hep G2 . 7.5% SDS PAGE. TA309009 diluted at 1:1000
Predicted band size: 95kDa. Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 10 minutes)
Predicted cell location: Cytoplasm. Positive control: Human tonsil tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human tonsil tissue using LRP12 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x200)
Predicted cell location: Cytoplasm. Positive control: Human ovarian and cervical cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using LRP12 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x 200)
Predicted cell location: Cytoplasm. Positive control: Human ovarian and cervical cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using LRP12 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x200)
Western blot analysis of lysate from SW480 cell line,using LRP12 Antibody (C-term)(Cat. #TA324430).TA324430 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.
LRP12 Antibody (C-term) (Cat. #TA324430)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LRP12 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of LRP12 Antibody (C-term)(Cat#TA324430) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
LRP12 Antibody (C-term) (Cat. #TA324430) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA309009 | LRP12 / ST7 antibody |
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| Rabbit polyclonal antibody to LRP12 (low density lipoprotein-related protein 12) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | WB |
0.1 ml /
€416.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA323871 | LRP12 / ST7 antibody |
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| Anti-LRP12 rabbit polyclonal antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA323872 | LRP12 / ST7 antibody |
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| Anti-LRP12 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA324430 | LRP12 / ST7 (C-term) antibody |
||||||
| Rabbit polyclonal LRP12 Antibody (C-term) | |||||||
 |
Rabbit | Ig | Aff - Purified | Hu | F, ICC/IF, P, WB |
0.4 ml /
€410.00
|
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
|||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN204847 | LRP12 - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN204847BN | LRP12 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN204847LP | LRP12 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN204847RB | LRP12 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN309427 | Lrp12 - mouse gene knockout kit via CRISPR |
||
|
Mouse | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN309427BN | Lrp12 - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN309427LP | Lrp12 - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN309427RB | Lrp12 - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP720653 | LRP12 / ST7 (transcript variant 1) |
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| Purified recombinant protein of Human low density lipoprotein receptor-related protein 12 (LRP12), transcript variant 1 | |||||
|
Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
10 µg /
€139.00
|
|
| OriGene Technologies, Inc. | |||||
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC204847L2V | Lenti ORF particles, LRP12 (mGFP-tagged)-Human low density lipoprotein receptor-related protein 12 (LRP12), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,875.00
|
|
| OriGene Technologies, Inc. | ||
| RC226705L2V | Lenti ORF particles, LRP12 (mGFP-tagged)-Human low density lipoprotein receptor-related protein 12 (LRP12), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,844.00
|
|
| OriGene Technologies, Inc. | ||
| RC204847L1V | Lenti ORF particles, LRP12 (Myc-DDK-tagged)-Human low density lipoprotein receptor-related protein 12 (LRP12), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,690.00
|
|
| OriGene Technologies, Inc. | ||
| RC226705L1V | Lenti ORF particles, LRP12 (Myc-DDK-tagged)-Human low density lipoprotein receptor-related protein 12 (LRP12), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,659.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR210912L2V | Lenti ORF particles, Lrp12 (GFP-tagged) - Mouse low density lipoprotein-related protein 12 (cDNA clone MGC:66539 IMAGE:6822657), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,432.00
|
|
| OriGene Technologies, Inc. | ||
| MR224979L2V | Lenti ORF particles, Lrp12 (GFP-tagged) - Mouse low density lipoprotein-related protein 12 (Lrp12), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,638.00
|
|
| OriGene Technologies, Inc. | ||
| MR210912L1V | Lenti ORF particles, Lrp12 (Myc-DDK-tagged) - Mouse low density lipoprotein-related protein 12 (cDNA clone MGC:66539 IMAGE:6822657), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,288.00
|
|
| OriGene Technologies, Inc. | ||
