LRPAP1 / A2MRAP antibody
Principal name
LRPAP1 / A2MRAP antibody
Alternative names for LRPAP1 / A2MRAP antibody
Alpha-2-MRAP, RAP, Alpha-2-macroglobulin receptor-associated protein, Low density lipoprotein receptor-related protein-associated protein 1
Ncbi ID
NP_002328.1, NP_002328, 9606, XP_001058156, NP_001162584, NP_038615, NP_038615.2, NM_002337
Available reactivities
Available hosts
Available applications
Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E)
Background of LRPAP1 / A2MRAP antibody
- Kit Component:
- - KN309434G1, Lrpap1 gRNA vector 1 in pCas-Guide vector
- KN309434G2, Lrpap1 gRNA vector 2 in pCas-Guide vector
- KN309434D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
1. Bajari, T.M., et al., (1998), “A minimal binding domain of the low density lipoprotein receptor family”,
Biol. Chem., 379, 1053-1062.
Human kidney; LRPAP1 antibody - C-terminal region (ARP45276_T100) in Human kidney cells using Immunohistochemistry
Human Jurkat; WB Suggested Anti-LRPAP1 Antibody Titration: 2.5ug/ml. Positive Control: Jurkat cell lysate; LRPAP1 antibody - C-terminal region (ARP45276_T100) in Human Jurkat cells using Western Blot
The lysate of Mouse kidney(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LRPAP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Predicted band size: 41 kDa . Positive control: 293T cell and human fetal kidney tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: 293T cell lysate Lane2: Human fetal kidney tissue lysate Lysates: 40 ug per lane Primary antibody: 1/1600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes)
Predicted cell location: Cytoplasm. Positive control: Human colon cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using LRPAP1 antibody at dilution 1/100, on the right is treated with the synthetic peptide. (Original magnification:x200)
LRPAP1 Antibody (C-term) (Cat. #TA325042) western blot analysis in Hela,HepG2 cell line lysates (35ug/lane).This demonstrates the LRPAP1 antibody detected the LRPAP1 protein (arrow).
Formalin-fixed and paraffin-embedded human brain tissue with LRPAP1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of HepG2 cells using LRPAP1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA323026 | LRPAP1 / A2MRAP antibody |
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| Anti-LRPAP1 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA325042 | LRPAP1 / A2MRAP (C-term) antibody |
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| Rabbit polyclonal LRPAP1 Antibody (C-term) | |||||||
 |
Rabbit | Hu | WB |
0.4 ml /
€410.00
|
|||
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| AM31641AF-N | LRPAP1 / A2MRAP antibody |
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 |
Mouse | IgG | 7F1 | Azide Free | Hu | WB |
1 mg /
€890.00
|
| Acris Antibodies GmbH | |||||||
| AM50345PU-N | LRPAP1 / A2MRAP antibody |
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 |
Mouse | IgG1 | AT8G8 | Purified | Hu | E, WB |
0.1 ml /
€310.00
|
| Acris Antibodies GmbH | |||||||
| AM50345PU-S | LRPAP1 / A2MRAP antibody |
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|
Mouse | IgG1 | AT8G8 | Purified | Hu | E, WB |
50 µl /
€220.00
|
| Acris Antibodies GmbH | |||||||
| BM5054 | LRPAP1 / A2MRAP antibody |
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|
Mouse | IgG1 | 7F1 | Purified | Hu | C, ICC/IF, WB |
50 µg /
€330.00
|
| Acris Antibodies GmbH | |||||||
| BP5016 | LRPAP1 / A2MRAP antibody |
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|
Rabbit | Serum | Chk, Hu, Rt | C, WB |
0.25 ml /
€310.00
|
||
| Acris Antibodies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN211255 | LRPAP1 - human gene knockout kit via CRISPR |
||
|
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN211255BN | LRPAP1 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN211255LP | LRPAP1 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN211255RB | LRPAP1 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN309434 | Lrpap1 - mouse gene knockout kit via CRISPR |
||
|
Mouse | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN309434BN | Lrpap1 - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN309434LP | Lrpap1 - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN309434RB | Lrpap1 - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP311255 | LRPAP1 / A2MRAP |
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| Recombinant protein of human low density lipoprotein receptor-related protein associated protein 1 (LRPAP1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€438.00
|
|
| OriGene Technologies, Inc. | |||||
|
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| AR09364PU-L | LRPAP1 / A2MRAP (His-tagged, myc-tagged) |
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|
Rat | Purified | > 95 % determined by SDS gelelectrophoresis | E. coli |
0.5 mg /
€860.00
|
| Acris Antibodies GmbH | |||||
| AR09364PU-N | LRPAP1 / A2MRAP (His-tagged, myc-tagged) |
||||
|
Rat | Purified | > 95 % determined by SDS gelelectrophoresis | E. coli |
0.25 mg /
€550.00
|
| Acris Antibodies GmbH | |||||
| AR09364PU-S | LRPAP1 / A2MRAP (His-tagged, myc-tagged) |
||||
|
Rat | Purified | > 95 % determined by SDS gelelectrophoresis | E. coli |
0.1 mg /
€360.00
|
| Acris Antibodies GmbH | |||||
| AR50209PU-N | LRPAP1 / A2MRAP (35-357, His-tag) |
||||
 |
Human | Purified | > 90 % by SDS - PAGE | E. coli |
0.5 mg /
€730.00
|
| Acris Antibodies GmbH | |||||
