LRPAP1 / A2MRAP antibody
Principal name
LRPAP1 / A2MRAP antibody
Alternative names for LRPAP1 / A2MRAP antibody
Alpha-2-MRAP, RAP, Alpha-2-macroglobulin receptor-associated protein, Low density lipoprotein receptor-related protein-associated protein 1
Ncbi ID
NP_002328.1, NP_002328, 9606, XP_001058156, NP_001162584, NP_038615
Available reactivities
Available hosts
Available applications
Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Flow Cytometry (F)
Background of LRPAP1 / A2MRAP antibody
Alpha-2-macroglobulin receptor-associated protein, also known as low density lipoprotein receptor-related protein-associated protein 1, RAP and LRPAP1, is a 39 kDa protein and a member of the alpha-2-MRAP family. LRPAP1 is a receptor antagonist that interacts with several members of the low density lipoprotein (LDL) receptor gene family. Upon binding to these receptors, LRPAP1 inhibits all ligand interactions with the receptors. LRPAP1 is present on cell surface forming a complex with the alpha-2-macroglobulin receptor heavy and light chains.LRPAP1 is present on the cell surface, where it is an effective inhibitor of ligand binding to the kidney glycoprotein 330 (gp330) yet it is most abundant in the endoplasmic reticulum lumen and may function in receptor folding and/or trafficking.
General readings
1. Bajari, T.M., et al., (1998), “A minimal binding domain of the low density lipoprotein receptor family”,
Biol. Chem., 379, 1053-1062.
Human kidney; LRPAP1 antibody - C-terminal region (ARP45276_T100) in Human kidney cells using Immunohistochemistry
Human Jurkat; WB Suggested Anti-LRPAP1 Antibody Titration: 2.5ug/ml. Positive Control: Jurkat cell lysate; LRPAP1 antibody - C-terminal region (ARP45276_T100) in Human Jurkat cells using Western Blot
Western Blot: LRPAP1 Lysate [NBL1-12673] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for LRPAP1
Western blot analysis of LRPAP1 Antibody (C-term) (AP18102PU-N) in A2058 cell line lysates (35ug/lane). LRPAP1 (arrow) was detected using the purified Pab.
Sample (30 ug of whole cell lysate) A: HeLa B: HepG2 10% SDS PAGE GTX104033 diluted at 1:1000
Western Blot: LRPAP1 Antibody [NBP1-69362] - This Anti-LRPAP1 antibody was used in Western Blot of Jurkat tissue lysate at a concentration of 2.5ug/ml.
Immunohistochemistry-Paraffin: LRPAP1 Antibody [NBP1-89334] - staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunofluorescence: LRPAP1 Antibody [NBP1-89334] - 2 OS shows positivity in endoplasmic reticulum.
Western Blot: LRPAP1 Antibody [NBP1-89334] Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 | Lane 2: RT-4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil
Formalin-fixed and paraffin-embedded human brain tissue with LRPAP1 Antibody (C-term)(GTX81193), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of HepG2 cells using LRPAP1 Antibody (C-term)(bottom histogram)(GTX81193) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
LRPAP1 Antibody (C-term) (GTX81193) western blot analysis in Hela,HepG2 cell line lysates (35ug/lane).This demonstrates the LRPAP1 antibody detected the LRPAP1 protein (arrow).
Immunohistochemistry of paraffin-embedded human kidney tissue slide using 24662-1-AP( LRPAP1 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human kidney tissue slide using 24662-1-AP( LRPAP1 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using 24662-1-AP( LRPAP1 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using 24662-1-AP( LRPAP1 Antibody) at dilution of 1:50 (under 40x lens)
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 24662-1-AP( LRPAP1 Antibody) at dilution of 1:1000
HeLa cells were subjected to SDS PAGE followed by western blot with 24662-1-AP( LRPAP1 Antibody) at dilution of 1:1000
1X10^6 HeLa cells were stained with 0.2ug LRPAP1 antibody (24662-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
The lysate of Mouse kidney(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LRPAP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA323026 | LRPAP1 / A2MRAP antibody |
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€325.00
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| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA323027 | LRPAP1 / A2MRAP antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA325042 | LRPAP1 / A2MRAP (C-term) antibody |
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 |
Rabbit | Hu | WB |
0.4 ml /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| AM31641AF-N | LRPAP1 / A2MRAP antibody |
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Mouse | IgG | 7F1 | Azide Free | Hu | WB |
1 mg /
€820.00
|
| Acris Antibodies GmbH | |||||||
| AM50345PU-N | LRPAP1 / A2MRAP antibody |
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 |
Mouse | IgG1 | AT8G8 | Purified | Hu | E, WB |
0.1 ml /
€310.00
|
| Acris Antibodies GmbH | |||||||
| AM50345PU-S | LRPAP1 / A2MRAP antibody |
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|
Mouse | IgG1 | AT8G8 | Purified | Hu | E, WB |
50 µl /
€220.00
|
| Acris Antibodies GmbH | |||||||
| BM5054 | LRPAP1 / A2MRAP antibody |
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Mouse | IgG1 | 7F1 | Purified | Hu | C, ICC/IF, WB |
50 µg /
€320.00
|
| Acris Antibodies GmbH | |||||||
| BP5016 | LRPAP1 / A2MRAP antibody |
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Rabbit | Serum | Chk, Hu, Rt | C, WB |
0.25 ml /
€300.00
|
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| Acris Antibodies GmbH | |||||||
| MAB3819 | LRPAP1 / A2MRAP antibody |
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Mouse | IgG | MA7F1 | Hu | WB |
1 mg /
€820.00
|
|
| Abnova Taiwan Corp. | |||||||
| MAB4328 | LRPAP1 / A2MRAP antibody |
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Mouse | IgG | 7F1 | Hu | WB |
1 mg /
€900.00
|
|
| Abnova Taiwan Corp. | |||||||
| ARP45276_T100 | LRPAP1 / A2MRAP antibody |
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 |
Rabbit | Aff - Purified | African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt | P, WB |
0.1 mg /
€310.00
|
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| AVIVA Systems Biology | |||||||
| +1 additional image |
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| NBP1-69362 | LRPAP1 / A2MRAP antibody |
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![Western Blot: LRPAP1 Antibody [NBP1-69362] - This Anti-LRPAP1 antibody was used in Western Blot of Jurkat tissue lysate at a concentration of 2.5ug/ml.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/LRPAP1 Antibody-Western Blot-NBP1-69362-img0001.jpg) |
Rabbit | Purified | Can, Hu, Ms, Rt | P, WB |
0.1 mg /
€360.00
|
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| Novus Biologicals Inc. | |||||||
| NBP1-89334 | LRPAP1 / A2MRAP antibody |
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![Immunohistochemistry-Paraffin: LRPAP1 Antibody [NBP1-89334] - staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/LRPAP1 Antibody-Immunohistochemistry-Paraffin-NBP1-89334-0005.jpg) |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 ml /
€500.00
|
|
| Novus Biologicals Inc. | |||||||
| +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP311255 | LRPAP1 / A2MRAP |
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
Regular Price: 20 µg / €680.00 Special Price: 20 µg / €398.00 |
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| OriGene Technologies, Inc. | |||||
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| AR09364PU-L | LRPAP1 / A2MRAP (His-tagged, myc-tagged) |
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Rat | Purified | > 95 % determined by SDS gelelectrophoresis | E. coli |
0.5 mg /
€840.00
|
| Acris Antibodies GmbH | |||||
| AR09364PU-N | LRPAP1 / A2MRAP (His-tagged, myc-tagged) |
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Rat | Purified | > 95 % determined by SDS gelelectrophoresis | E. coli |
0.25 mg /
€540.00
|
| Acris Antibodies GmbH | |||||
| AR09364PU-S | LRPAP1 / A2MRAP (His-tagged, myc-tagged) |
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Rat | Purified | > 95 % determined by SDS gelelectrophoresis | E. coli |
0.1 mg /
€350.00
|
| Acris Antibodies GmbH | |||||
| AR31040PU-N | LRPAP1 / A2MRAP |
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Rat | Purified | > 95 % by SDS-PAGE. | E. coli |
0.1 mg /
€610.00
|
| Acris Antibodies GmbH | |||||
| AR50209PU-N | LRPAP1 / A2MRAP (35-357, His-tag) |
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 |
Human | Purified | > 90 % by SDS - PAGE | E. coli |
0.5 mg /
€730.00
|
| Acris Antibodies GmbH | |||||
| AR50209PU-S | LRPAP1 / A2MRAP (35-357, His-tag) |
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|
Human | Purified | > 90 % by SDS - PAGE | E. coli |
0.1 mg /
€300.00
|
| Acris Antibodies GmbH | |||||
