LRRC50 antibody
Principal name
LRRC50 antibody
Alternative names for LRRC50 antibody
Leucine-rich repeat-containing protein 50
Gene ID
123872 (DNAAF1)
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F)
Background of LRRC50 antibody
The protein encoded by this gene is cilium-specific and is required for the stability of the ciliary architecture. It is involved in the regulation of microtubule-based cilia and actin-based brush border microvilli. Mutations in this gene are associated with primary ciliary dyskinesia-13. COMPLETENESS: complete on the 3' end.
General readings
Kimura,K., (2006) Genome Res. 16 (1), 55-65
Human 721_B; WB Suggested Anti-LRRC50 Antibody Titration: 0.2-1 ug/ml. Positive Control: 721_B cell lysate. DNAAF1 is supported by BioGPS gene expression data to be expressed in 721_B.; LRRC50 antibody - N-terminal region (ARP53359_P050) in Human 721_B cells using Western Blot
Western Blot: LRRC50 Lysate [NBL1-12700] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for LRRC50
Human 721_B; Host:Rabbit. Target Name:DNAAF1. Sample Tissue:Human 721_B. Antibody Dilution: 1.0ug/ml. DNAAF1 is supported by BioGPS gene expression data to be expressed in 721_B.; LRRC50 antibody - N-terminal region (ARP53358_P050) in Human 721_B cells using Western Blot
Hum. Fetal Lung; Host:Rabbit. Target Name:DNAAF1. Sample Tissue:Human Fetal Lung. Antibody Dilution: 1.0ug/ml.; LRRC50 antibody - N-terminal region (ARP53358_P050) in Hum. Fetal Lung cells using Western Blot
Hum. Fetal Muscle; Host:Rabbit. Target Name:DNAAF1. Sample Tissue:Human Fetal Muscle. Antibody Dilution: 1.0ug/ml.; LRRC50 antibody - N-terminal region (ARP53358_P050) in Hum. Fetal Muscle cells using Western Blot
Human HepG2; WB Suggested Anti-LRRC50 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: HepG2 cell lysate; LRRC50 antibody - N-terminal region (ARP53358_P050) in Human HepG2 cells using Western Blot
Immunohistochemistry-Paraffin: LRRC50 Antibody [NBP1-85005] - Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts.
Immunofluorescence: LRRC50 Antibody [NBP1-85005] - 2 OS shows positivity in nucleus, plasma membrane & cytoplasm.
Western Blot: LRRC50 Antibody [NBP1-85005] Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT-4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil
Sample (50 ug of whole cell lysate) A: mouse testis 7.5% SDS PAGE GTX122620 diluted at 1:1000
Detection of LRRC50 in 721_B using anti-LRRC50 antibody (GTX45545) at 1 µg/mL.
Western blot analysis of Human Fetal Lung using LRRC50 antibody, N-term (GTX45545)
Western blot analysis of Human Fetal Liver using LRRC50 antibody, N-term (GTX45545)
Detection of LRRC50 in HepG2 using anti-LRRC50 antibody (GTX45546) at 1 µg/mL.
Anti-LRRC50 mouse monoclonal antibody (TA504535) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LRRC50(RC205452).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LRRC50 (RC205452, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LRRC50.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LRRC50 (RC205452, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LRRC50.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-LRRC50 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-LRRC50 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LRRC50 (RC205452, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LRRC50.
HEK293T cells transfected with either RC205452 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LRRC50 antibody(TA504546), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LRRC50 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LRRC50 (RC205452, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LRRC50.
HEK293T cells transfected with either RC205452 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LRRC50 antibody(TA504574), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LRRC50 (RC205452, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LRRC50.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LRRC50 (RC205452, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LRRC50.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LRRC50 (RC205452, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LRRC50.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LRRC50 (RC205452, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LRRC50.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-LRRC50 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA337574 | LRRC50 antibody |
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Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Por, Rt, Ze | WB |
50 µg /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
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| ARP53358_P050 | LRRC50 antibody |
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 |
Rabbit | Aff - Purified | Bov, Can, Chk, Hu, Ms, Rt | WB |
50 µg /
€350.00
|
||
| AVIVA Systems Biology | |||||||
| +3 additional images |
Highly validated antibody! |
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| TA337575 | LRRC50 antibody |
||||||
 |
Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Por, Rt | WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA504535 | LRRC50 antibody |
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 |
Mouse | IgG1 | OTI4E4 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA504536 | LRRC50 antibody |
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 |
Mouse | IgG1 | OTI3B10 | Purified | Can, Hu | WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA504546 | LRRC50 antibody |
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 |
Mouse | IgG1 | OTI2C4 | Purified | Can, Hu, Mky, Ms, Rt | F, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA504574 | LRRC50 antibody |
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 |
Mouse | IgG1 | OTI3F9 | Purified | Hu, Mky, Ms, Rt | F, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA504575 | LRRC50 antibody |
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 |
Mouse | IgG2b | OTI3A1 | Purified | Hu | WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA504576 | LRRC50 antibody |
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 |
Mouse | IgG1 | OTI5F8 | Purified | Hu | WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA504577 | LRRC50 antibody |
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 |
Mouse | IgG2b | OTI3E2 | Purified | Hu | WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA504578 | LRRC50 antibody |
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 |
Mouse | IgG1 | OTI4H3 | Purified | Hu, Mky, Ms, Rt | WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| ARP53359_P050 | LRRC50 antibody |
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 |
Rabbit | Aff - Purified | African clawed frog, Bov, Can, Chk, Hu, Ms, Rt | WB |
50 µg /
€350.00
|
||
| AVIVA Systems Biology | |||||||
| NBP1-85005 | LRRC50 antibody |
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![Immunohistochemistry-Paraffin: LRRC50 Antibody [NBP1-85005] - Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/LRRC50 Antibody-Immunohistochemistry-Paraffin-NBP1-85005-0005.jpg) |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€500.00
|
|
| Novus Biologicals Inc. | |||||||
| +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP305452 | LRRC50 |
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
Regular Price: 20 µg / €680.00 Special Price: 20 µg / €398.00 |
|
| OriGene Technologies, Inc. | |||||
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Lysates (Overexpressed)
| Catalog No. | ||
|---|---|---|
| LY405926 | DNAAF1 overexpression lysate |
|
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0.1 mg /
€280.00
|
|
| OriGene Technologies, Inc. |
