LRRC50 antibody
Principal name
LRRC50 antibody
Alternative names for LRRC50 antibody
Leucine-rich repeat-containing protein 50
Ncbi ID
NP_848547, NP_848547.4, NM_178452, NP_080924.1, NM_026648, NM_001014154
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)
Background of LRRC50 antibody
- Kit Component:
- - KN304655G1, Dnaaf1 gRNA vector 1 in pCas-Guide vector
- KN304655G2, Dnaaf1 gRNA vector 2 in pCas-Guide vector
- KN304655D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Kimura,K., (2006) Genome Res. 16 (1), 55-65
Human 721_B; WB Suggested Anti-LRRC50 Antibody Titration: 0.2-1 ug/ml. Positive Control: 721_B cell lysate. DNAAF1 is supported by BioGPS gene expression data to be expressed in 721_B.; LRRC50 antibody - N-terminal region (ARP53359_P050) in Human 721_B cells using Western Blot
Human 721_B; Host:Rabbit. Target Name:DNAAF1. Sample Tissue:Human 721_B. Antibody Dilution: 1.0ug/ml. DNAAF1 is supported by BioGPS gene expression data to be expressed in 721_B.; LRRC50 antibody - N-terminal region (ARP53358_P050) in Human 721_B cells using Western Blot
Hum. Fetal Lung; Host:Rabbit. Target Name:DNAAF1. Sample Tissue:Human Fetal Lung. Antibody Dilution: 1.0ug/ml.; LRRC50 antibody - N-terminal region (ARP53358_P050) in Hum. Fetal Lung cells using Western Blot
Hum. Fetal Muscle; Host:Rabbit. Target Name:DNAAF1. Sample Tissue:Human Fetal Muscle. Antibody Dilution: 1.0ug/ml.; LRRC50 antibody - N-terminal region (ARP53358_P050) in Hum. Fetal Muscle cells using Western Blot
Human HepG2; WB Suggested Anti-LRRC50 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: HepG2 cell lysate; LRRC50 antibody - N-terminal region (ARP53358_P050) in Human HepG2 cells using Western Blot
Anti-LRRC50 mouse monoclonal antibody (TA504535) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LRRC50(RC205452).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LRRC50 (RC205452, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LRRC50.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LRRC50 (RC205452, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LRRC50.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-LRRC50 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-LRRC50 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LRRC50 (RC205452, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LRRC50.
HEK293T cells transfected with either RC205452 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LRRC50 antibody(TA504546), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LRRC50 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LRRC50 (RC205452, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LRRC50.
HEK293T cells transfected with either RC205452 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LRRC50 antibody(TA504574), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LRRC50 (RC205452, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LRRC50.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LRRC50 (RC205452, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LRRC50.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LRRC50 (RC205452, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LRRC50.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LRRC50 (RC205452, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LRRC50.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-LRRC50 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
WB Suggested Anti-DNAAF1Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: HepG2 cell lysate
Host: Rabbit; Target Name: DNAAF1; Sample Tissue: Human Fetal Muscle; Antibody Dilution: 1.0 ug/ml
Host: Rabbit; Target Name: DNAAF1; Sample Tissue: Human Fetal Lung; Antibody Dilution: 1.0 ug/ml
Host: Rabbit; Target Name: DNAAF1; Sample Tissue: Human 721_B; Antibody Dilution: 1.0 ug/ml DNAAF1 is supported by BioGPS gene expression data to be expressed in 721_B
WB Suggested Anti-DNAAF1Antibody Titration: 0.2-1 ug/ml; Positive Control: 721_B cell lysate DNAAF1 is supported by BioGPS gene expression data to be expressed in 721_B
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA337574 | LRRC50 antibody |
||||||
| Rabbit Polyclonal Anti-DNAAF1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Por, Rt, Ze | WB |
50 µg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
||||||
| TA337575 | LRRC50 antibody |
||||||
| Rabbit Polyclonal Anti-DNAAF1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Por, Rt | WB |
50 µg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA504535 | LRRC50 antibody |
||||||
| LRRC50 mouse monoclonal antibody, clone OTI4E4 (formerly 4E4) | |||||||
 |
Mouse | IgG1 | OTI4E4 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
||||||
| TA504536 | LRRC50 antibody |
||||||
| LRRC50 mouse monoclonal antibody, clone OTI3B10 (formerly 3B10) | |||||||
 |
Mouse | IgG1 | OTI3B10 | Purified | Can, Hu | WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
||||||
| TA504546 | LRRC50 antibody |
||||||
| LRRC50 mouse monoclonal antibody, clone OTI2C4 (formerly 2C4) | |||||||
 |
Mouse | IgG1 | OTI2C4 | Purified | Can, Hu, Mky, Ms, Rt | F, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA504574 | LRRC50 antibody |
||||||
| LRRC50 mouse monoclonal antibody, clone OTI3F9 (formerly 3F9) | |||||||
 |
Mouse | IgG1 | OTI3F9 | Purified | Hu, Mky, Ms, Rt | F, WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA504575 | LRRC50 antibody |
||||||
| LRRC50 mouse monoclonal antibody, clone OTI3A1 (formerly 3A1) | |||||||
 |
Mouse | IgG2b | OTI3A1 | Purified | Hu | WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA504576 | LRRC50 antibody |
||||||
| LRRC50 mouse monoclonal antibody, clone OTI5F8 (formerly 5F8) | |||||||
 |
Mouse | IgG1 | OTI5F8 | Purified | Hu | WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA504577 | LRRC50 antibody |
||||||
| LRRC50 mouse monoclonal antibody, clone OTI3E2 (formerly 3E2) | |||||||
 |
Mouse | IgG2b | OTI3E2 | Purified | Hu | WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
|
|||||||
| TA504578 | LRRC50 antibody |
||||||
| LRRC50 mouse monoclonal antibody, clone OTI4H3 (formerly 4H3) | |||||||
 |
Mouse | IgG1 | OTI4H3 | Purified | Hu, Mky, Ms, Rt | WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN205452 | DNAAF1 - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN205452BN | DNAAF1 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN205452LP | DNAAF1 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN205452RB | DNAAF1 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN304655 | Dnaaf1 - mouse gene knockout kit via CRISPR |
||
|
Mouse | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN304655BN | Dnaaf1 - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN304655LP | Dnaaf1 - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN304655RB | Dnaaf1 - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP305452 | LRRC50 |
||||
| Recombinant protein of human leucine rich repeat containing 50 (LRRC50) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€438.00
|
|
| OriGene Technologies, Inc. | |||||
|
|||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY405926 | DNAAF1 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
