Luciferase antibody

Principal name

Luciferase antibody

Alternative names for Luciferase antibody

Luciferin 4-monooxygenase

SwissProt ID

P08659 (Phopy), P09140 (Phole)

Ncbi ID

7054, 553611

Available reactivities

Pp (Photinus pyralis), Photobacterium fischerii, Bac (Bacteria), Hu (Human)

Available hosts

Mouse, Goat, Rabbit, Chicken

Available applications

Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Frozen Sections (C), Enzyme Immunoassay (E), Immunoprecipitation (IP), Dot blot (Dot), Immunodiffusion (ID), Radioimmunoassay (R)

Background of Luciferase antibody

Analysis of gene expression is most commonly assayed by transient transfection. These systems are usually based on the use of fusion genes which are inserted into cells, and expression of the gene is assayed within 48 hours after introduction of DNA. Usually the fusion consists of the promoter binding site or enhancer sequence under study which is attached to a reporter gene. The amount of the reporter protein synthesized under the experimental conditions, is presumed to reflect the ability of the sequences studied to direct or promote transcription. Several enzymes are commonly used as reporter proteins, among them are chloramphenicol acetyl transferase (CAT), â-galactosidase, human growth hormone (hGH), and luciferase. Luciferase has become one of the more widely used reporter enzymes. The reporter plasmid contains the gene from the firefly Photinus pyralis. The enzyme catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg+2 and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. The luciferase assay is fast and sensitive and does not require a radioactive substrate as is in the CAT assay. A disadvantage of the luciferase assay is that it requires a rather expensive instrument, the luminometer, to measure enzyme activity. In addition, this assay lacks reproducibility between samples, largely due to the rapid kinetics of the emission.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Luciferase (Photinus pyralis) (C-term) antibody

Luciferase Goat IgG Aff - Purified WB
0.6 mg / €300.00
  SICGEN

Luciferase (Photinus pyralis) (C-term) antibody

Luciferase Goat IgG Aff - Purified WB
1.5 mg / €450.00
  SICGEN

Luciferase (Photinus pyralis) antibody

Luciferase Mouse IgG1 Luci17 Purified C, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

Luciferase (Photinus pyralis) antibody

Luciferase Mouse IgG1 BGN/06/944 Purified E
0.2 mg / €400.00
  Acris Antibodies GmbH

Luciferase antibody

Western blot analysis of Luciferase expression in pG L3/293T cells using AM26631AF-N. Mouse IgG1 2D4 Azide Free Pp IP, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Luciferase (Photinus pyralis) antibody

Luciferase Goat IgG Biotin E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Luciferase (Photinus pyralis) antibody

Luciferase Goat IgG FITC F, ICC/IF
0.1 mg / €440.00
  Acris Antibodies GmbH

Luciferase (Photinus pyralis) antibody

Luciferase Goat IgG HRP E, WB
0.1 ml / €420.00
  Acris Antibodies GmbH

Luciferase (Photinus pyralis) antibody

Luciferase Goat IgG Purified C, E, ICC/IF, P, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

Luciferase antibody

Luciferase Rabbit IgG Azide Free Pp Dot, E, ICC/IF, WB
10 mg / €230.00
  Acris Antibodies GmbH

Luciferase antibody

Luciferase Rabbit IgG Biotin Pp Dot, E, ICC/IF, ID, IP, R, WB
1 ml / €340.00
  Acris Antibodies GmbH

Luciferase antibody

Luciferase Rabbit IgG Aff - Purified Pp Dot, E, ICC/IF, ID, IP, R, WB
1 mg / €430.00
  Acris Antibodies GmbH

Luciferase (Photobacterium) antibody

Luciferase Rabbit IgG Azide Free Photobacterium fischerii Dot, E, ICC/IF, ID, IP, R, WB
10 mg / €230.00
  Acris Antibodies GmbH

Luciferase (Photobacterium) antibody

Luciferase Rabbit IgG Biotin Photobacterium fischerii Dot, E, ICC/IF, ID, IP, R, WB
1 ml / €340.00
  Acris Antibodies GmbH

Luciferase (Photinus pyralis) antibody

Luciferase Goat Serum C, E, IP, WB
2 ml / €440.00
  Acris Antibodies GmbH

Luciferase (Photobacterium) antibody

Luciferase Rabbit Serum Photobacterium fischerii E, WB
2 ml / €1,150.00
  Acris Antibodies GmbH

Luciferase (Photinus pyralis) antibody

Luciferase Mouse IgG1 LUC-Y Purified C, E, F, WB
0.1 mg / €630.00
  Acris Antibodies GmbH

Luciferase antibody

Luciferase
0.1 mg / €400.00
  Abnova Taiwan Corp.

Luciferase antibody

Detection of luciferase expression in CD34+ cells by immunohistochemistry.
Cytospin slides prepared from transduced CD34+ cells after 3 days of culture were stained with Luciferase monoclonal antibody, clone Luci 21 1-107 ( Cat # MAB2322 ).
Luciferase-positive cells have green cytoplasm; nuclei stained with DAPI are blue.
Nontransduced, cultured CD34+ cells were used as a negative control.
Original magnification, x 40.
Wang, X. et al., Dynamic tracking of human hematopoietic stem cell. Blood. 102 ( 10 ) : 3478-3482, 2003. Mouse IgG1 Luci 21 1-107 ICC/IF, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

Luciferase (Photinus pyralis) antibody

Luciferase Goat C, E, ICC/IF, WB
0.5 mg / €400.00
  Abnova Taiwan Corp.

Items 1 to 20 of 32 total

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