Ly6A/E / SCA1 antibody

Principal name

Ly6A/E / SCA1 antibody

Alternative names for Ly6A/E / SCA1 antibody

Lymphocyte antigen 6A-2/6E-1, Ly-6A.2/Ly-6E.1, T-cell-activating protein, TAP, Stem cell antigen 1, SCA-1

SwissProt ID

P05533 (Mouse), Q63316 (Rat)

Gene ID

110454 (Ly6a)

Available reactivities

Ms (Mouse)

Available hosts

Mouse, Rat, Rabbit

Available applications

Cytotoxicity Assay (CT), Flow Cytometry (F), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Frozen Sections (C), Paraffin Sections (P), Enzyme Immunoassay (E)

Background of Ly6A/E / SCA1 antibody

Sca-1 is a member of the Ly-6 antigen family with molecular mass of 8 kDa under non-reducing conditions and 18 kDa under reducing conditions. Mouse hematopoietic stem cells express low levels of Thy-1 antigen (Thy-1lo) and to be negative for lineage markers (Lin-) that is, not expressing markers characteristic of B cells (B220), granulocytes (Gr-1), myelomonocytic cells (Mac-1) and T lymphocytes (CD4 and CD8). Recently, a new monoclonal antibody, anti-Sca-1, was used to purify stem cells from the Thy-1lo, Lin- bone marrow subpopulation. Thy-1lo, Lin-, Sca-1+ (but not the Thy-1lo, Lin-, Sca-1-) population of bone marrow cells are highly purified pluripotent stem cells. They read out with nearly unit efficiency in assays for primitive myeloerythroid and thymic progenitor, and have a capability to admit lethally irradiated mouse to survive and be restored in all blood-cell lineages. The Thy-1lo, Lin-, Sca-1+ subpopulation thought to have all stem cells present in the bone marrow.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Ly6A/E / SCA1 antibody

  Rabbit Polyclonal Anti-Ly6a Antibody  
Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane: Mouse skin tissue, Primary antibody: (Ly6a Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes Rabbit IgG Purified Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Ly6A/E / SCA1 antibody

  Rat Monoclonal anti-Ly-6A/E (Sca-1) Antibody  
C57BL/6 splenocytes stained with purified D7, followed by anti-rat IgG FITC Rat D7 Purified Ms F, IP, P, WB
50 µg / €248.00
  OriGene Technologies, Inc.

Ly6A/E / SCA1 antibody

Ly6A/E / SCA1 Rat IgG2a D7 APC Ms F
100 TESTS/1ml / €450.00
  Acris Antibodies GmbH

Ly6A/E / SCA1 antibody

Ly6A/E / SCA1 Rat IgG2a D7 Biotin Ms C, F, IP, WB
0.1 mg / €300.00
  Acris Antibodies GmbH

Ly6A/E / SCA1 antibody

Ly6A/E / SCA1 Rat IgG2a D7 FITC Ms F
0.1 mg / €300.00
  Acris Antibodies GmbH

Ly6A/E / SCA1 antibody

Ly6A/E / SCA1 Rat IgG2a D7 Purified Ms F, IP, WB
0.25 mg / €410.00
  Acris Antibodies GmbH

Ly6A/E / SCA1 antibody

Ly6A/E / SCA1 Rat IgG2a D7 PE-Cy5 Ms F
0.1 mg / €650.00
  Acris Antibodies GmbH

Ly6A/E / SCA1 antibody

Ly6A/E / SCA1 Rat IgG2a D7 PE Ms F
100 TESTS/1ml / €330.00
  Acris Antibodies GmbH

Ly6A/E / SCA1 antibody

Flow cytometric analysis of mouse Sca-1 expression on LO cells. Open histogram indicates the reaction of isotypic control to the cells. Shaded histogram indicates the reaction of AM26430AF-N to the cells. Rat IgG2a 238B Azide Free Ms F
0.1 mg / €390.00
  Acris Antibodies GmbH

Ly6A/E / SCA1 antibody

Flow cytometric analysis of Biotin labeled mouse S ca-1 expression on LO cells. Open histogram indicates the reaction of Isotypic control to the cells. Shaded histogram indicates the reaction of AM26430BT-N to the cells. Rat IgG2a 238B Biotin Ms F
0.1 ml / €320.00
  Acris Antibodies GmbH

Ly6A/E / SCA1 antibody

Flow cytometric analysis of Mouse Sca-1 expression on Jurkat cells (left) and LO cells (right). Open histograms indicate the reaction of isotypic control to the cells. Shaded histograms indicate the reaction of AM26430RP-N to the cells. Rat IgG2a 238B PE Ms F
50 Tests / €350.00
  Acris Antibodies GmbH

Ly6A/E / SCA1 antibody

Ly6A/E / SCA1 Rat IgG2b CT-6A/6E Purified Ms F
0.2 mg / €430.00
  Acris Antibodies GmbH

Ly6A/E / SCA1 antibody

Ly6A/E / SCA1 Rat IgG2b CT-6A/6E PE Ms F
0.3 mg / €1,200.00
  Acris Antibodies GmbH

Ly6A/E / SCA1 antibody

Ly6A/E / SCA1 Rat IgG2b CT-6A/6E PE Ms F
50 µg / €340.00
  Acris Antibodies GmbH

Ly6A/E / SCA1 antibody

Ly6A/E / SCA1 Mouse IgG2a 1147447 Ascites Ms CT
0.5 ml / €500.00
  Acris Antibodies GmbH

Ly6A/E / SCA1 antibody

BALB/c spleen cells were activated by incubation for 48 hours with concanavalin-A. After harvesting, the cells were stained with Ly6a monoclonal antibody, clone D7 ( Cat # MAB3239 )-FITC, and activated cells were gated and analyzed on a FACScanTM flow cytometer. Rat IgG2a D7 Ms C, F, IP, WB
0.5 mg / €400.00
  Abnova Taiwan Corp.

CRISPR

Catalog No. Species Donor Vector  

Ly6a - mouse gene knockout kit via CRISPR

Ly6a - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ly6a - mouse gene knockout kit via CRISPR

Ly6a - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

18 Item(s)

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