M-CSF / CSF-1 antibody

Principal name

M-CSF / CSF-1 antibody

Alternative names for M-CSF / CSF-1 antibody

CSF1, MCSF, Macrophage colony-stimulating factor 1, Macrophage Colony Stimulating Factor

SwissProt ID

P07141 (Mouse), P09603 (Human), Q12150 (Yeast), Q8JZQ0 (Rat)

Gene ID

12977 (Csf1), 1435 (CSF1)

Available reactivities

Ms (Mouse), Hu (Human), Rt (Rat), Can (Canine), GP (Guinea Pig), Rb (Rabbit), Bov (Bovine), Por (Porcine)

Available hosts

Rat, Rabbit, Mouse, Goat

Available applications

Frozen Sections (C), Flow Cytometry (F), Paraffin Sections (P), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of M-CSF / CSF-1 antibody

Four distinct colony-stimulating factors (CSFs) that promote survival, proliferation and differentiation of bone marrow precursor cells have been well characterized: granulocyte macrophage CSF (GMCSF), granulocyte CSF (GCSF), macrophage CSF (MCSF), and Interleukin-3 (IL-3, Multi CSF). Both GMCSF and IL-3 are multipotential growth factors, stimulating proliferation of progenitor cells from more than one hematopoietic lineage. In contrast, GCSF and MCSF are lineage restricted hematopoietic growth factors, stimulating final mitotic divisions and the terminal cellular maturation of the partially differentiated hematopoietic progenitors.
Macrophage CSF, also known as CSF1, is produced by monocytes, fibroblasts and endothelial cells. It stimulates the formation of macrophage colonies, enhances antibody-dependent, cell-mediated cytotoxicity by monocytes and macrophages, and inhibits bone resorption by osetoclasts. Natural human MCSF is a dimeric glycoprotein of 70-90 kD molecular weight, existing in multiple glycosylation forms. It binds to a 165 kD glycoprotein of the receptor tyrosine kinase subclass III, a family that includes the receptors for platelet derived growth factor (PDGF) and stem cell factor (SCF).

General readings

1. Ladner, M. B.; Martin, G. A.; Noble, J. A.; Nikoloff, D. M.; Tal, R.; Kawasaki, E. S.; White, T. J. :
Human CSF-1: gene structure and alternative splicing of mRNA precursors. EMBO J. 6: 2693-2698, 1987.
2. Kawasaki, E. S.; Ladner, M. B.; Wang, A. M.; Van Arsdell, J.; Warren, M. K.; Coyne, M. Y.; Schweickart, V. L.; Lee, M.-T.; Wilson, K. J.; Boosman, A.; Stanley, E. R.; Ralph, P.; Mark, D. F. : Molecular cloning of a complementary DNA encoding human macrophage-specific colony-stimulating factor (CSF-1). Science 230: 291-296, 1985.
3. Ladner, M. B.; Martin, G. A.; Noble, J. A.; Nikoloff, D. M.; Tal, R.; Kawasaki, E. S.; White, T. J. : Human CSF-1: gene structure and alternative splicing of mRNA precursors. EMBO J. 6: 2693-2698, 1987.
4. Pollard, J. W.; Bartocci, A.; Arceci, R.; Orlofsky, A.; Ladner, M. B.; Stanley, E. R. : Apparent role of the macrophage growth factor, CSF-1, in placental development. Nature 330: 484-486, 1987.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

M-CSF / CSF-1 antibody

  Rabbit Polyclonal MCSF Antibody  
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-CSF1 rabbit polyclonal antibody. (TA590307) Rabbit IgG Purified Hu, Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

M-CSF / CSF-1 antibody

  Biotinylated Anti-Human M-CSF Rabbit Polyclonal Antibody  
To detect hM-CSF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hM-CSF. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

M-CSF / CSF-1 antibody

  Anti-Human M-CSF Rabbit Polyclonal Antibody  
To detect hM-CSF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human M-CSF (TA328372) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hM-CSF. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

M-CSF / CSF-1 antibody

  Biotinylated Anti-Murine M-CSF Goat Polyclonal Antibody  
To detect mM-CSF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine M-CSF (TA328531) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mM-CSF. Goat IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

M-CSF / CSF-1 antibody

  Anti-Murine M-CSF Goat Polyclonal Antibody  
To detect mM-CSF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine M-CSF (TA328530) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mM-CSF. Goat IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

M-CSF / CSF-1 antibody

  Rabbit Polyclonal Anti-CSF1 Antibody  
WB Suggested Anti-CSF1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Can, GP, Hu, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

M-CSF / CSF-1 antibody

  Rabbit Polyclonal Anti-CSF1 Antibody  
WB Suggested Anti-CSF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: COLO205 cell lysate Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Por, Rt ICC/IF, P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

M-CSF / CSF-1 antibody

  Rabbit Polyclonal Anti-CSF1 Antibody  
WB Suggested Anti-CSF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: COLO205 cell lysate Rabbit IgG Purified Can, GP, Hu, Ms, Por, Rt ICC/IF, P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

M-CSF / CSF-1 antibody

  Mouse Monoclonal anti-M-CSF Antibody  
Immunoprecipitation/Western blot analysis using the M-CSF antibody (L168B11). Supernatant of M-CSF overexpressing cells was tested at a 1:40 dilution. Lane 1 was immunoprecipitated with mouse IgG control antibody and lane 2 was immunoprecipitated with M-CSF antibody (L168B11). Western blot analysis was performed using goat anti-human M-CSF antibody. Mouse IgG3 L168B11 Purified Hu IP
0.5 mg / €379.00
  OriGene Technologies, Inc.

M-CSF / CSF-1 antibody

  CSF1 mouse monoclonal antibody, clone OTI1D10 (formerly 1D10)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CSF1 mouse monoclonal antibody. (TA806556; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min) Mouse IgG1 OTI1D10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

M-CSF / CSF-1 antibody

  CSF1 mouse monoclonal antibody, clone OTI8E1 (formerly 8E1)  
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CSF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806567) Mouse IgG2b OTI8E1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

M-CSF / CSF-1 antibody

  CSF1 mouse monoclonal antibody, clone OTI2D6 (formerly 2D6)  
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CSF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806568) Mouse IgG1 OTI2D6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

M-CSF / CSF-1 antibody

  CSF1 mouse monoclonal antibody, clone OTI1F8 (formerly 1F8)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSF1 (RC217104, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSF1. Mouse IgG1 OTI1F8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

M-CSF / CSF-1 antibody

Immunohistochemical analysis of paraffin-embedded human Placenta tissues using anti-CSF1 mouse mAb Mouse IgG1 2D10 Ascites Hu E, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

M-CSF / CSF-1 antibody

M-CSF / CSF-1 Mouse IgG2b 116 Purified Hu E
0.5 mg / €460.00
  Acris Antibodies GmbH

M-CSF / CSF-1 antibody

M-CSF / CSF-1 Mouse IgG2a 692 Aff - Purified Hu E
0.5 mg / €460.00
  Acris Antibodies GmbH

M-CSF / CSF-1 antibody

M-CSF / CSF-1 Mouse IgG2a 21 Aff - Purified Hu E
0.5 mg / €460.00
  Acris Antibodies GmbH

M-CSF / CSF-1 antibody

Western blot analysis of M-CSF (arrow) using rabbit polyclonal M-CSF Antibody (Center) (AP17554PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MCSF gene (Lane 2) (Origene Technologies). Rabbit Hu WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

M-CSF / CSF-1 antibody

BM4089 ER-MP58 antibody staining of Mouse Spleen Frozen Section. Rat IgM ER-MP58 Partially Purified Ms C, F, P
1 ml / €500.00
  Acris Antibodies GmbH

M-CSF / CSF-1 antibody

M-CSF / CSF-1 Rat IgG2a ER-MP58 Purified Ms C, F
0.1 mg / €350.00
  Acris Antibodies GmbH

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