MAD1L1 antibody

Principal name

MAD1L1 antibody

Alternative names for MAD1L1 antibody

Mitotic spindle assembly checkpoint protein MAD1, MAD1-like 1, Mitotic arrest deficient-like protein 1, Mitotic checkpoint MAD1 protein homolog, Tax-binding protein 181

SwissProt ID

Q80YF0 (Crigr), Q9WTX8 (Mouse), Q9Y6D9 (Human)

Gene ID

8379 (MAD1L1), 837906 (AT1G13460)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Paraffin Sections (P), Immunoprecipitation (IP), PLA/Proximity ligation assay (PLA), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of MAD1L1 antibody

Mitotic checkpoint prevents the transition of eukaryotic cells to anaphase until all the chromosomes have made productive, bipolar attachments through their kinetochores to the microtubules of the mitotic spindle. In Saccharomyces cervisiae six genes are important for the kinetochore-dependent mitotic checkpoint: MAD1, MAD2, MAD3, BUB1, BUB3, and Spindles gene MPS1. All six genes have homologues in higher eukaryotes.1-3 In mammals the mitotic checkpoint is an important mechanism that controls the advance to anaphase during every mitosis. Gene inactivation of MAD2 or Bub3 causes lethality in mice and acquisition of aneuploidy in cell culture. Unattached kinetochores are responsible for generating the checkpoint signal that prevents progression to the anaphase stage. Part of the signal is to inhibit the Cdc20-activated form of a ubiquitin ligase, inhibition of the anaphase promoting complex/cyclosome (APC/C) and inhibition of the ubiquitination of substrates whose destruction is important for the cell to advance to the anaphase stage. Human Mad1 can complex with Mad2 and is preferentially localized to unattached kinetochores during mitosis. Both Mad1 and Mad2 localize to the nuclear pore complex throughout interphase.1-3

General readings

Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Guo, Y., et al. J. Med. Genet. 47(9):616-622(2010)
Wang, H.B., et al. J. Gastrointest. Surg. 14(8):1227-1234(2010)
Hewitt, L., et al. J. Cell Biol. 190(1):25-34(2010)
Ge, Z., et al. FASEB J. 24(2):579-586(2010)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MAD1L1 antibody

Western blot analysis of extracts of various cell lines, using MAD1L1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAD1L1 antibody

Host: Rabbit; Target Name: MAD1L1; Sample Tissue: Placenta lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

MAD1L1 (N-term) antibody

Western blot analysis of MAD1L1 Antibody (N-term) Cat.-No AP52579PU-N in MDA-MB231 cell line lysates (35ug/lane). This demonstrates the MAD1L1 antibody detected the MAD1L1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MAD1L1 antibody

MAD1L1 Hu
0.1 mg / €560.00
  Abnova Taiwan Corp.

MAD1L1 antibody

MAD1L1 Rabbit Hu, Ms IP, WB
0.1 ml / €400.00
  Abnova Taiwan Corp.

MAD1L1 pSer428 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MAD1 (Phospho-Ser428) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, P
50 µg / €205.00
  Assay Biotech

MAD1L1 pSer428 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MAD1 (Phospho-Ser428) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, P
0.1 mg / €310.00
  Assay Biotech

MAD1L1 antibody

Immunofluorescence analysis of NIH-3T3 cells, using MAD1 (Ab-428) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF
50 µg / €205.00
  Assay Biotech

MAD1L1 antibody

Immunofluorescence analysis of NIH-3T3 cells, using MAD1 (Ab-428) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF
0.1 mg / €310.00
  Assay Biotech

MAD1L1 antibody

Mad1 Antibody [9B10] Mouse IgG2b 9B10 Purified Hu ICC/IF, IP
0.1 ml / €400.00
  Immuquest

MAD1L1 antibody

Mad1 Antibody [9B10] Mouse IgG2b 9B10 Purified Hu ICC/IF, IP
50 µl / €360.00
  Immuquest

MAD1L1 antibody

Immunohistochemistry-Paraffin: MAD1 Antibody [NB100-569] - FFPE section of human prostate adenocarcinoma.  Affinity purified rabbit anti-MAD1 used at a dilution of 1:250.  Detection: DAB staining using Immunohistochemistry Accessory Kit Rabbit Aff - Purified Hu IP, P, PLA, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

MAD1L1 antibody

Western Blot: MAD1 Antibody [NB100-570] - Whole cell lysate (50 ug/lane) from HeLa cells were treated with Nocodazole (+) or mock treated (-) and probed with anti-MAD1 antibody at 0.1 ug/ml. Rabbit Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

MAD1L1 antibody

MAD1L1 Mouse IgG2b 9B10 Aff - Purified Hu ICC/IF, IP
0.1 ml / €450.00
  Novus Biologicals Inc.

MAD1L1 antibody

Immunohistochemistry-Paraffin: MAD1 Antibody [NBP1-32775] - Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using Mad 1  antibody at 1:100 dilution. Rabbit IgG Hu P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MAD1L1 (transcript variant 1)

MAD1L1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

MAD1L1 (transcript variant 2)

MAD1L1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

MAD1L1

MAD1L1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

MAD1L1

MAD1L1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

MAD1L1 overexpression lysate

MAD1L1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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