MAD2L1 antibody

Principal name

MAD2L1 antibody

Alternative names for MAD2L1 antibody

Mitotic spindle assembly checkpoint protein MAD2A, MAD2

SwissProt ID

Q13257 (Human), Q9Z1B5 (Mouse)

Gene ID

4085 (MAD2L1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Mky (Monkey), Can (Canine), Por (Porcine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Immunoprecipitation (IP), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of MAD2L1 antibody

The spindle checkpoint ensures the fidelity of chromosome segregation in mitosis and meiosis. In response to defects in the mitotic apparatus, it blocks the activity of the anaphase-promoting complex, a large ubiquitin ligase required for chromosome segregation. Recent studies indicate that the spindle checkpoint monitors both the attachment of chromosomes to the mitotic spindle and the tension across the sister chromatid generated by microtubules. Upon checkpoint activation, checkpoint protein complexes containing BubR1(Mad3), Bub3, Mad2 and Cdc20 directly bind to the anaphase-promoting complex and inhibit its ligase activity. Therefore, the checkpoint proteins form a complex intracellular signalling network to inhibit the anaphase-promoting complex.

General readings

1. Wei, J. H., et al., J. Biol. Chem. 280, 7748-7757 (2005).
2. Sudakin, V., et al., J. Cell Biol. 154, 925-936 (2001).
Clone 2G9 is used in reference number 1).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MAD2L1 antibody

Sample (30ug whole cell lysate). A:Hep G2 . B:MOLT4 . C:Raji . 12% SDS PAGE. TA308923 diluted at 1:1000 Rabbit IgG Aff - Purified Hu ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAD2L1 antibody

Western blot analysis of extracts of various cell lines, using MAD2L1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAD2L1 antibody

TA305640 (2ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Bov, Can, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAD2L1 antibody

Sample (30 ug of whole cell lysate). A: 293T. 12% SDS PAGE. TA308922 diluted at 1:1000. Rabbit IgG Aff - Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAD2L1 antibody

Western blot analysis of extracts from A549 cells, using MAD2L1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAD2L1 antibody

WB using Anti-MAD2L1 antibody shows detection of a predominant band at ~24 kDa corresponding to MAD2L1 (arrowhead) present in Jurkat (lane 1) and HeLa (lane 2) whole cell lysates(green). Specific band reactivity is blocked when the antibody is pre-incubated with immunizing peptide (lanes 4 and 5 respectively). The primary antibody was diluted to 1:1200. IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] was used at 1:10000. Rabbit Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAD2L1 antibody

WB using anti-MAD2L1 antibody shows detection of a band at ~24 kDa (arrowhead) corresponding to MAD2L1 present in a HeLa whole cell lysate (lane 1). Approximately 75 µg of lysate was separated by 4-20% TG SDS-PAGE. After blocking, the membrane was probed overnight at 4? with the primary antibody diluted to 1:200. The membrane was washed and reacted with a 1:5,000 dilution of IRDye™800 conjugated Sh-a-Mouse IgG [H&L] for 45 min at RT. Mouse IgG1 17D10 Purified Hu IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAD2L1 antibody

Predicted band size: 24 kDa . Positive control: Human seminoma tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/100 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAD2L1 antibody

WB Suggested Anti-MAD2L1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: MCF7 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

MAD2L1 antibody

Anti-MAD2L1 mouse monoclonal antibody (TA500617) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAD2L1(RC203273). Mouse IgG2a OTI4D2 Purified Hu ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

MAD2L1 antibody

Western blot analysis of MAD2 expression in Jurkat (Lane 1), HeLa (Lane 2), Raji (Lane 3), WR19L (Lane 4) and PC12 (Lane 5) using AM20288AF-N MAD2 antibody. Mouse IgG1 2G9 Azide Free Hu, Ms, Rt IP, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

MAD2L1 antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Brain, cortex. Rabbit Aff - Purified Chk, Hu, Ms E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

MAD2L1 (1-174) antibody

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µl / €470.00
  Acris Antibodies GmbH

MAD2L1 Control Peptide

MAD2L1 Purified
50 µg / €190.00
  Acris Antibodies GmbH

MAD2L1 antibody

MAD2L1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

MAD2L1 antibody

Western Blot analysis of MAD2L1 expression in transfected 293T cell line (H00004085-T02) by MAD2L1 MaxPab polyclonal antibody.

Lane 1: MAD2L1 transfected lysate(23.50 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

MAD2L1 antibody

MAD2L1 monoclonal antibody (M01), clone 2E2-1D6 Western Blot analysis of MAD2L1 expression in HeLa ( Cat # L013V1 ). Mouse IgG2b 2E2-1D6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MAD2L1 antibody

Western blot using MAD2L1 monoclonal antibody, clone 17D10 ( Cat # MAB1926 ) shows detection of aband at ~24 kDa ( arrowhead ) correspondingto MAD2L1 present in a HeLa whole cell lysate ( lane 1 ).
Approximately 75 µg of lysate was separated by 4-20% TG SDS-PAGE.
After blocking, the membrane was probed overnight at 4°C with the primary antibody diluted to 1:200.
The membrane was washed and reacted with a 1 : 5,000 dilution of IRDyeTM800 conjugated Sh-a-Mouse IgG [H&L] for 45 minat room temperature ( 800 nm channel, green ).
Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
IRDyeTM800 fluorescence image was captured using the Odyssey® InfraredImaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc. Otherdetection systems will yield similar results. Hu
0.1 mg / €560.00
  Abnova Taiwan Corp.

MAD2L1 antibody

MAD2L1 Goat Hu E
0.1 mg / €400.00
  Abnova Taiwan Corp.

MAD2L1 antibody

Western blot using MAD2L1 polyclonal antibody ( Cat # PAB10076 ) shows detection of a predominant band at ~24 kDa corresponding to MAD2L1 ( arrowhead ) present in Jurkat ( lane 1 ) and HeLa ( lane 2 ) whole cell lysates using the 800 nmchannel ( green ).
The identity of the higher molecular weight bands is unknown, although they may represent complexes of MAD2L1 with related binding proteins.
Specific band reactivity is blocked when the antibody is pre-incubated with immunizingpeptide ( lanes 4 and 5 respectively ) which completely blocks antibody staining.
Approximately 35 µg of lysate was separated on a 4-20% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1:1200.
Incubation was 2h at room temperature followed by washes andreaction with a 1:10,000 dilution of IRDyeTM800 conjugated Gt-a-Rabbit IgG [H&L] MXHu for 45 min at room temperature.
Molecular weight markers were used for size comparison using the 700 nm channel ( lane 3 ).  IRDyeTM800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc. Hu
0.1 mg / €560.00
  Abnova Taiwan Corp.

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