MAD2L1 antibody

Principal name

MAD2L1 antibody

Alternative names for MAD2L1 antibody

Mitotic spindle assembly checkpoint protein MAD2A, MAD2

SwissProt ID

Q13257 (Human), Q9Z1B5 (Mouse)

Gene ID

4085 (MAD2L1), 56150 (Mad2l1)

Available reactivities

Hu (Human), Chk (Chicken), Ms (Mouse), Rt (Rat), Mky (Monkey), Bov (Bovine), Can (Canine), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of MAD2L1 antibody

Kit Component:
- KN309637G1, Mad2l1 gRNA vector 1 in pCas-Guide vector
- KN309637G2, Mad2l1 gRNA vector 2 in pCas-Guide vector
- KN309637D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

1. Wei, J. H., et al., J. Biol. Chem. 280, 7748-7757 (2005).
2. Sudakin, V., et al., J. Cell Biol. 154, 925-936 (2001).
Clone 2G9 is used in reference number 1).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MAD2L1 antibody

  Rabbit Polyclonal antibody to Mad2L1 (MAD2 mitotic arrest deficient-like 1 (yeast))  
Sample (30ug whole cell lysate). A:Hep G2 . B:MOLT4 . C:Raji . 12% SDS PAGE. TA308923 diluted at 1:1000 Rabbit IgG Aff - Purified Hu ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAD2L1 antibody

  Rabbit anti-MAD2L1 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using MAD2L1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAD2L1 antibody

  Goat Anti-MAD2L1 Antibody  
TA305640 (2ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Bov, Can, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAD2L1 antibody

  Rabbit polyclonal antibody to MAD2 (MAD2 mitotic arrest deficient-like 1 (yeast))  
Sample (30 ug of whole cell lysate). A: 293T. 12% SDS PAGE. TA308922 diluted at 1:1000. Rabbit IgG Aff - Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAD2L1 antibody

  Rabbit polyclonal anti-MAD2L1 antibody  
Western blot analysis of extracts from A549 cells, using MAD2L1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAD2L1 antibody

  Rabbit polyclonal anti-MAD2L1 antibody  
WB using Anti-MAD2L1 antibody shows detection of a predominant band at ~24 kDa corresponding to MAD2L1 (arrowhead) present in Jurkat (lane 1) and HeLa (lane 2) whole cell lysates(green). Specific band reactivity is blocked when the antibody is pre-incubated with immunizing peptide (lanes 4 and 5 respectively). The primary antibody was diluted to 1:1200. IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] was used at 1:10000. Rabbit Aff - Purified Hu E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

MAD2L1 antibody

  Mouse monoclonal MAD2L1 antibody  
WB using anti-MAD2L1 antibody shows detection of a band at ~24 kDa (arrowhead) corresponding to MAD2L1 present in a HeLa whole cell lysate (lane 1). Approximately 75 µg of lysate was separated by 4-20% TG SDS-PAGE. After blocking, the membrane was probed overnight at 4? with the primary antibody diluted to 1:200. The membrane was washed and reacted with a 1:5,000 dilution of IRDye™800 conjugated Sh-a-Mouse IgG [H&L] for 45 min at RT. Mouse IgG1 17D10 Purified Hu IP, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

MAD2L1 antibody

  Anti-MAD2L1 Rabbit Polyclonal Antibody  
Predicted band size: 24 kDa . Positive control: Human seminoma tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/100 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAD2L1 antibody

  Rabbit Polyclonal Anti-MAD2L1 Antibody  
WB Suggested Anti-MAD2L1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: MCF7 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

MAD2L1 antibody

  MAD2L1 (MAD2) mouse monoclonal antibody, clone OTI4D2 (formerly 4D2)  
Anti-MAD2L1 mouse monoclonal antibody (TA500617) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAD2L1(RC203273). Mouse IgG2a OTI4D2 Purified Hu ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

MAD2L1 antibody

Western blot analysis of MAD2 expression in Jurkat (Lane 1), HeLa (Lane 2), Raji (Lane 3), WR19L (Lane 4) and PC12 (Lane 5) using AM20288AF-N MAD2 antibody. Mouse IgG1 2G9 Azide Free Hu, Ms, Rt IP, WB
0.1 mg / €400.00
  Acris Antibodies GmbH

MAD2L1 antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Brain, cortex. Rabbit Aff - Purified Chk, Hu, Ms E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

MAD2L1 (1-174) antibody

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µl / €470.00
  Acris Antibodies GmbH

MAD2L1 Control Peptide

MAD2L1 Purified
50 µg / €200.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

Mad2l1 - mouse gene knockout kit via CRISPR

Mad2l1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Mad2l1 - mouse gene knockout kit via CRISPR

Mad2l1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Mad2l1 - mouse gene knockout kit via CRISPR

Mad2l1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Mad2l1 - mouse gene knockout kit via CRISPR

Mad2l1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MAD2L1

  Recombinant protein of human MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1)  
MAD2L1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

MAD2L1 (1-205, His-tag)

MAD2L1 Human Purified > 95 % by SDS - PAGE E. coli
50 µg / €730.00
  Acris Antibodies GmbH

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