MAD2L1-binding protein antibody

Principal name

MAD2L1-binding protein antibody

Alternative names for MAD2L1-binding protein antibody

MAD2L1BP, CMT2

SwissProt ID

Q15013 (Human), Q9DCX1 (Mouse)

Gene ID

9587 (MAD2L1BP), 269587 (Epb4.1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Chicken, Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)

Background of MAD2L1-binding protein antibody

The protein encoded by this gene was identified as a binding protein of the MAD2 mitotic arrest deficient-like 1 (MAD2/MAD2L1). MAD2 is a key component of the spindle checkpoint that delays the onset of anaphase until all the kinetochores are attached to the spindle. This protein may interact with the spindle checkpoint and coordinate cell cycle events in late mitosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. Transcript Variant: This variant differs in the 5' region compared to variant 1, which includes a part of the coding sequence. The resulting isoform (2) has a distinct and shorter N-terminus, as compared to isoform 1.

General readings

1. Rual,J.F., Venkatesan,K., Hao,T., Hirozane-Kishikawa,T., Dricot,A., Li,N., Berriz,G.F., Gibbons,F.D., Dreze,M., Ayivi-Guedehoussou,N.,, et al.
Towards a proteome-scale map of the human protein-protein interaction network
2. Xia,G., Luo,X., Habu,T., Rizo,J., Matsumoto,T. and Yu,H., et al.
Conformation-specific binding of p31(comet) antagonizes the function of Mad2 in the spindle checkpoint
3. Habu,T., Kim,S.H., Weinstein,J. and Matsumoto,T., et al.
Identification of a MAD2-binding protein, CMT2, and its role in mitosis
4. Hoja,M.R., Wahlestedt,C. and Hoog,C., et al.
A visual intracellular classification strategy for uncharacterized human proteins
5. , Nagase T., Miyajima N., Tanaka A., Sazuka T., Seki N., Sato S., Tabata S., Ishikawa K., Kawarabayasi Y., Kotani H., et al.
Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1.
6. , Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., et al.
The DNA sequence and analysis of human chromosome 6.
7. , Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., et al.
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
8. , Habu T., Kim S.H., Weinstein J., Matsumoto T.
Identification of a MAD2-binding protein, CMT2, and its role in mitosis.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MAD2L1-binding protein antibody

Western blot analysis of extracts from K562 cells, using MAD2L1BP antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAD2L1-binding protein antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MAD2L1BP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804847) Mouse IgG2b OTI1C11 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAD2L1-binding protein antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAD2L1BP (RC202640, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAD2L1BP. Mouse IgG1 OTI1D1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAD2L1-binding protein antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MAD2L1BP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804860) Mouse IgG2b OTI3C11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAD2L1-binding protein (N-term) antibody

Western blot analysis of MAD2L1BP Antibody (N-term) Cat.-No AP52580PU-N in MCF-7 cell line lysates (35ug/lane). This demonstrates the MAD2L1BP antibody detected the MAD2L1BP protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MAD2L1-binding protein antibody

Immunofluorescence of purified MaxPab antibody to MAD2L1BP on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

MAD2L1-binding protein antibody

Immunofluorescence of purified MaxPab antibody to MAD2L1BP on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

MAD2L1-binding protein antibody

Western Blot analysis of MAD2L1BP expression in transfected 293T cell line (H00009587-T02) by MAD2L1BP MaxPab polyclonal antibody.

Lane 1: MAD2L1BP transfected lysate(34.70 KDa).
Lane 2: Non-transfected lysate. Rabbit Serum Hu, Ms E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

MAD2L1-binding protein antibody

MAD2L1BP MaxPab rabbit polyclonal antibody. Western Blot analysis of MAD2L1BP expression in mouse liver. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

MAD2L1-binding protein antibody

MAD2L1BP monoclonal antibody (M03), clone 4G11. Western Blot analysis of MAD2L1BP expression in Hela NE ( Cat # L013V3 ). Mouse IgG2a 4G11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MAD2L1-binding protein antibody

MAD2L1-binding protein Mouse IgG2a 4B9 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

MAD2L1-binding protein (N-term) antibody

Human MDA-MB-435S; WB Suggested Anti-MAD2L1BP Antibody. . Titration: 1.0 ug/ml. . Positive Control: MDA-MB-435S Whole Cell. MAD2L1BP is supported by BioGPS gene expression data to be expressed in MDA-MB435.; MAD2L1BP antibody - N-terminal region (ARP61356_P050) in Human MDA-MB-435S cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

MAD2L1-binding protein antibody

Western blot analysis of MAD2L1BP expression in HEK293T (A); Raw264.7 (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

MAD2L1-binding protein antibody

Western blot analysis of MAD2L1BP expression in HEK293T (A); Raw264.7 (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

MAD2L1-binding protein antibody

Western blot analysis of MAD2L1BP expression in HEK293T (A); Raw264.7 (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

MAD2L1-binding protein antibody

Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-MAD2L1BP ) Chicken Aff - Purified Hu WB
50 µg / €520.00
  Novus Biologicals Inc.

MAD2L1-binding protein antibody

MAD2L1-binding protein Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MAD2L1-binding protein (transcript variant 2)

MAD2L1-binding protein Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

MAD2L1-binding protein (full length, N-term HIS tag, transcript variant 1)

MAD2L1-binding protein Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

MAD2L1-binding protein (1-274, His-tag)

MAD2L1-binding protein Human Purified > 90 % by SDS - PAGE E. coli
0.25 mg / €730.00
  Acris Antibodies GmbH

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