Hu (Human), Rt (Rat), Bov (Bovine), Ms (Mouse), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit)
Mouse, Rabbit, Rat
Frozen Sections (C), Flow Cytometry (F), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB)
Background of Major Vault Protein antibody
MVP is identical to lung-resistance related protein (LRP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Amino acid 241-280 of human estrogen receptor (ER), (site of nuclear localization signal sequence), is mapped to be the site of interaction between ER and MVP. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. Anti-estrogen 1C1182 shows no effect. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to progesterone and glucocorticoid receptors are also able to co-immunoprecipitate the MVP. LRP is a protein overexpressed in many neoplastic tissues and cell lines. Expression of LRP predicts a poor response to chemotherapy. This 104-kD protein is the major vault protein (MVP) also described as the lung resistance protein (LRP) and has shown to interact with the estrogen receptor. The protein is part of a very large vault ribonucleoprotein complex present in all eukaryotic cells and its structure and protein composition is highly conserved. Because of the size, shape, and protein and RNA composition of this complex the particles are different from other ribonucleoproteins.
Immunoperoxidase of monoclonal antibody to MVP on formalin-fixed paraffin-embedded human lung, adenosqumous cell carcinoma. [antibody concentration 3ug/ml]
Western blot analysis of MVP ( arrow ) using rabbit MVP polyclonal antibody ( Cat # PAB4800 ) . 293 cell lysate ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the MVP gene ( Lane 2 ) ( Origene Technologies ) .
Human COLO205; WB Suggested Anti-MVP Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: COLO205 cell lysate; MVP antibody - N-terminal region (ARP58642_P050) in Human COLO205 cells using Western Blot
Western blot analysis of MVP (arrow) using rabbit polyclonal MVP C-term Antibody (Cat.#TA302181). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MVP gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human lung carcinoma with MVP Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of NCI-H292 cells using MVP Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
MVP/LRP antibody detects MVP protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug BCL-1 whole cell lysate/extract. E. 30 ug Raw264.7 whol
Predicted cell location: Cytoplasm. Positive control: Human lung cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using MVP antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification: x200)
Predicted cell location: Cytoplasm. Positive control: Human lung cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using MVP antibody at dilution 1/40, on the right is treated with the fusion protein. (Original magnification: x200)