Makorin-1 antibody

Principal name

Makorin-1 antibody

Alternative names for Makorin-1 antibody

MKRN1, RNF61, RING finger protein 61

SwissProt ID

Q9QXP6 (Mouse), Q9UHC7 (Human)

Gene ID

23608 (MKRN1)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Flow Cytometry (F), Paraffin Sections (P)

Background of Makorin-1 antibody

The Makorin ring finger protein-1 gene (MKRN1) is a highly transcribed, intron-containing source for a family of intronless mammalian genes encoding a novel class of zinc finger proteins. Phylogenetic analyses indicate that the MKRN1 gene is the ancestral founder of this gene family.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.

General readings

Knutson,M., (2004) J. Neurosci. Res. 76 (5), 633-641

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Makorin-1 antibody

  MKRN1 mouse monoclonal antibody, clone OTI2C8 (formerly 2C8)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MKRN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504092) Mouse IgG2b OTI2C8 Purified Hu F, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Makorin-1 antibody

HEK293 lysate (10ug protein in RIPA buffer) over expressing Human MKRN1 with DYKDDDDK tag probed with Makorin Antibody Cat.-No AP31559PU-N (1 µg/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed with AP31559PU-N (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Makorin-1 antibody

  Rabbit Polyclonal Anti-MKRN1 Antibody  
WB Suggested Anti-MKRN1 Antibody Titration: 1.25 ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Makorin-1 antibody

  Rabbit Polyclonal Anti-MKRN1 Antibody  
WB Suggested Anti-MKRN1 Antibody Titration: 1.25 ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Makorin-1 (105-118) antibody

  Goat Polyclonal Anti-MKRN1 (aa105-118) Antibody  
HEK293 lysate (10ug protein in RIPA buffer) over expressing Human MKRN1 with DYKDDDDK tag probed with TA348997 (0.3ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed with EB10898 (1mg/ml) in Lane B. Prim Goat Aff - Purified Bov, Can, Hu, Ms, Por E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Makorin-1 antibody

  MKRN1 mouse monoclonal antibody, clone OTI3F9 (formerly 3F9)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MKRN1 (RC206848, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MKRN1. Mouse IgG2b OTI3F9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Makorin-1 antibody

  MKRN1 mouse monoclonal antibody, clone OTI1G6 (formerly 1G6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MKRN1 (RC206848, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MKRN1. Mouse IgG2b OTI1G6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Makorin-1 antibody

Makorin-1 Rabbit Serum Hu C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Makorin-1 (transcript variant 1)

  Recombinant protein of human makorin ring finger protein 1 (MKRN1), transcript variant 1  
Makorin-1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Lysates

Catalog No.    

MKRN1 overexpression lysate

MKRN1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

MKRN1 overexpression lysate

MKRN1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, MKRN1 (mGFP-tagged) - Human makorin ring finger protein 1 (MKRN1), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, MKRN1 (mGFP-tagged) - Human makorin ring finger protein 1 (MKRN1), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €960.00
  OriGene Technologies, Inc.

Lenti ORF particles, MKRN1 (Myc-DDK tagged) - Human makorin ring finger protein 1 (MKRN1), transcript variant 2 , 200 uL, >10^7 TU/mL

Lenti ORF particles, MKRN1 (Myc-DDK tagged) - Human makorin ring finger protein 1 (MKRN1), transcript variant 2 , 200 uL, >10^7 TU/mL  
200 µl / €860.00
  OriGene Technologies, Inc.

13 Item(s)

per page
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