Makorin-1 antibody

Principal name

Makorin-1 antibody

Alternative names for Makorin-1 antibody

MKRN1, RNF61, RING finger protein 61

SwissProt ID

Q9QXP6 (Mouse), Q9UHC7 (Human)

Gene ID

23608 (MKRN1)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Frozen Sections (C), Flow Cytometry (F)

Background of Makorin-1 antibody

The Makorin ring finger protein-1 gene (MKRN1) is a highly transcribed, intron-containing source for a family of intronless mammalian genes encoding a novel class of zinc finger proteins. Phylogenetic analyses indicate that the MKRN1 gene is the ancestral founder of this gene family.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.

General readings

Knutson,M., (2004) J. Neurosci. Res. 76 (5), 633-641

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Makorin-1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MKRN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504092) Mouse IgG2b OTI2C8 Purified Hu F, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Makorin-1 antibody

HEK293 lysate (10ug protein in RIPA buffer) over expressing Human MKRN1 with DYKDDDDK tag probed with Makorin Antibody Cat.-No AP31559PU-N (1 µg/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed with AP31559PU-N (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Makorin-1 antibody

WB Suggested Anti-MKRN1 Antibody Titration: 1.25 ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Makorin-1 antibody

WB Suggested Anti-MKRN1 Antibody Titration: 1.25 ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Makorin-1 (105-118) antibody

HEK293 lysate (10ug protein in RIPA buffer) over expressing Human MKRN1 with DYKDDDDK tag probed with TA348997 (0.3ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed with EB10898 (1mg/ml) in Lane B. Prim Goat Aff - Purified Bov, Can, Hu, Ms, Por E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Makorin-1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MKRN1 (RC206848, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MKRN1. Mouse IgG2b OTI3F9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Makorin-1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MKRN1 (RC206848, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MKRN1. Mouse IgG2b OTI1G6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Makorin-1 antibody

Makorin-1 Rabbit Serum Hu C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

Makorin-1 antibody

Makorin-1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Makorin-1 antibody

Makorin-1 Mouse IgG2b 4E9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Makorin-1 antibody

Human Jurkat; WB Suggested Anti-MKRN1 Antibody Titration: 1.25ug/ml. Positive Control: Jurkat cell lysate; MKRN1 antibody - N-terminal region (ARP43141_T100) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

Makorin-1 antibody

Human Jurkat; WB Suggested Anti-MKRN1 Antibody Titration: 1.25ug/ml. Positive Control: Jurkat cell lysate; MKRN1 antibody - C-terminal region (ARP43142_T100) in Human Jurkat cells using Western Blot Rabbit Purified Can, Hu, Ms WB
0.1 mg / €310.00
  AVIVA Systems Biology

Makorin-1 antibody

Immunohistochemistry-Paraffin: MKRN1 Antibody [NB100-55304] - Please see notes section Rabbit Aff - Purified Hu IP, P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Makorin-1 antibody

Western Blot: MKRN1 Antibody [NBP1-55226] - This Anti-MKRN1 antibody was used in Western Blot of Jurkat tissue lysate at a concentration of 1.25ug/ml. Rabbit Purified Can, Hu, Ms, Rt WB
0.1 mg / €360.00
  Novus Biologicals Inc.

Makorin-1 antibody

Immunohistochemistry-Paraffin: MKRN1 Antibody [NBP1-87123] - Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Makorin-1 (transcript variant 1)

Makorin-1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Makorin-1

Makorin-1 Human Purified
2 µg / €300.00
  Abnova Taiwan Corp.

Makorin-1

Makorin-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Makorin-1

Makorin-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

MKRN1 overexpression lysate

MKRN1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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