Maltose Binding Protein Tag / MBP-Tag antibody

Principal name

Maltose Binding Protein Tag / MBP-Tag antibody

Alternative names for Maltose Binding Protein Tag / MBP-Tag antibody

MMBP, Maltodextrin-binding protein, malE-Tag

SwissProt ID

P0AEX9 (Ecoli)

Gene ID

948538 (malE)

Available reactivities

Hu (Human), E. coli (Escherichia coli), Bac (Bacteria)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (capture) (E(capture)), Paraffin Sections (P)

Background of Maltose Binding Protein Tag / MBP-Tag antibody

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

General readings

Fox JD., et al. (2001). Protein Sci. 10(3): 622-30
Riggs., et al. (2000). Mol. Biotechnol. 15: 51-63

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Maltose Binding Protein Tag / MBP-Tag antibody

Bacterial fusion protein immunoblot Mouse IgG2a N128A/4 Supernatant E, WB
5 ml / €320.00
  Antibodies Incorporated

NeuroMab

Maltose Binding Protein Tag / MBP-Tag antibody

Bacterial fusion protein immunoblot Mouse IgG2a N128A/4 Purified E, WB
0.1 ml / €380.00
  Antibodies Incorporated

NeuroMab

Maltose Binding Protein Tag / MBP-Tag antibody

Maltose Binding Protein Tag / MBP-Tag Goat IgG Aff - Purified E. coli WB
20 µl / €50.00
  SICGEN

Maltose Binding Protein Tag / MBP-Tag antibody

Maltose Binding Protein Tag / MBP-Tag Goat IgG Aff - Purified E. coli WB
0.6 mg / €300.00
  SICGEN

Maltose Binding Protein Tag / MBP-Tag antibody

Maltose Binding Protein Tag / MBP-Tag Goat IgG Aff - Purified E. coli WB
1.5 mg / €450.00
  SICGEN

Maltose Binding Protein Tag / MBP-Tag antibody

Antibody sensitivity: MBP was separated by SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with MBP antibody (6E4) Cat.-No AM00088PU-N (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). 
Lane 1: 100ng MBP
Lane 2: 50ng
Lane 3: 25ng 
Lane 4: 10ng 
Lane 5: 5ng 
Lane 6: 2ng 
Lane 7: 1ng Mouse IgG1 6E4 Purified E, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

Maltose Binding Protein Tag / MBP-Tag antibody

Western blot analysis: Recombinant protein MBP-tagged BCDEF (Lane 1), TPD52 (Lane 2) and MAGEA3 (Lane 3) (each 20 ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-MBP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 10C12 Purified E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Maltose Binding Protein Tag / MBP-Tag antibody

Western blot analysis: Recombinant protein MBP-tagged BCDEF (Lane 1), TPD52 (Lane 2) and MAGEA3 (Lane 3) (each 20 ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-MBP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 10C12 Purified E, WB
50 µl / €230.00
  Acris Antibodies GmbH

Maltose Binding Protein Tag / MBP-Tag antibody

Western blot analysis of MBP expression in pM AL-c2G/JM 109 E. coli lysate using AM26627AF-N. Mouse IgG3 1G12 Azide Free E. coli IP, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Maltose Binding Protein Tag / MBP-Tag antibody

Anti-MBP epitope tag polyclonal antibody detects MBP-tagged recombinant proteins by western blot. Polyclonal rabbit-anti-MBP epitope tag at 0.5-1.0 µg/ml was used to detect 1.0 ug of recombinant protein  containing the MBP epitope tag. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature. LICOR's Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. Rabbit IgG Purified E, WB
1 mg / €510.00
  Acris Antibodies GmbH

Maltose Binding Protein Tag / MBP-Tag Control Peptide

Maltose Binding Protein Tag / MBP-Tag
5 mg / €450.00
  Acris Antibodies GmbH

Maltose Binding Protein Tag / MBP-Tag antibody

Maltose Binding Protein Tag / MBP-Tag Mouse IgG1 R29 Purified IP, WB
0.25 mg / €450.00
  Acris Antibodies GmbH

Maltose Binding Protein Tag / MBP-Tag antibody

Maltose Binding Protein Tag / MBP-Tag Rabbit Serum WB
0.1 ml / €450.00
  Acris Antibodies GmbH

Maltose Binding Protein Tag / MBP-Tag antibody

Western blot analysis of recombinant protein MBP - tagged BCDEF ( lane 1 ) , TPD52 ( lane 2 ) and MAGEA3 ( lane 3 ) ( each 20 ng ) were resolved by SDS - PAGE , transferred to PVDF membrane and probed with MBP tag monoclonal antibody , clone 10C12 ( 1 : 1000 ) ( Cat # MAB1133 ) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 10C12 Hu E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

Maltose Binding Protein Tag / MBP-Tag antibody

Maltose Binding Protein Tag / MBP-Tag Mouse IgG1 R29.6 E. coli IP, WB
0.2 mg / €560.00
  Abnova Taiwan Corp.

Maltose Binding Protein Tag / MBP-Tag antibody

Maltose Binding Protein Tag / MBP-Tag Rabbit C, WB
0.2 mg / €390.00
  Abnova Taiwan Corp.

Maltose Binding Protein Tag / MBP-Tag antibody

Maltose Binding Protein Tag / MBP-Tag Rabbit IgG Hu E, ICC/IF, IP, WB
1 mg / €640.00
  Abnova Taiwan Corp.

Maltose Binding Protein Tag / MBP-Tag antibody

Maltose Binding Protein Tag / MBP-Tag Rabbit IgG E. coli E, E(capture), WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Maltose Binding Protein Tag / MBP-Tag antibody

Maltose Binding Protein Tag / MBP-Tag Rabbit IgG E. coli E, WB
1 mg / €730.00
  Abnova Taiwan Corp.

Maltose Binding Protein Tag / MBP-Tag antibody

Maltose Binding Protein Tag / MBP-Tag Rabbit IgG Serum WB
50 µl / €360.00
  Novus Biologicals Inc.

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