MAP-2 antibody

Principal name

MAP-2 antibody

Alternative names for MAP-2 antibody

Microtubule-associated protein 2, MAP2, Neuronal Marker

SwissProt ID

P11137 (Human), P15146 (Rat), P20357 (Mouse), Q28869 (Bovin)

Gene ID

4133 (MAP2)

Available reactivities

Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Bov (Bovine), Chk (Chicken), Quail, Xen (Xenopus), Prim (Primate), Av (Birds), Fe (Feline), Por (Porcine), Mam (Mammalia)

Available hosts

Mouse, Rabbit, Chicken

Available applications

Frozen Sections (C), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Enzyme Immunoassay (E)

Background of MAP-2 antibody

Microtubules are 25nm diameter protein rods found in most kinds of eukarytic cells. They are polymerized from a dimeric subunit made of one alpha subunit and one beta tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein tau and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels, and is expressed early in development. All these MAP2 forms are derived from a single gene by alterte transcription, and all share a C-termil sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein tau. MAP2 isoforms are expressed only in neurol cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neurol cells, their perikarya and neurol dendrites. In contrast tau is found predomintly in neurol axons.

General readings

1. Fontaine-Lenoir, V.; Chambraud, B.; Fellous, A.; David, S.; Duchossoy, Y.; Baulieu, E.-E.; Robel, P. :
Microtubule-associated protein 2 (MAP2) is a neurosteroid receptor. Proc. Nat. Acad. Sci. 103: 4711-4716, 2006.
2. Neve, R. L.; Harris, P.; Kosik, K. S.; Kurnit, D. M.; Donlon, T. A. : Identification of cDNA clones for the human microtubule-associated protein tau and chromosomal localization of the genes for tau and microtubule-associated protein 2. Molec. Brain Res. 1: 271-280, 1986.
3. Kalcheva, N.; Albala, J.; O'Guin, K.; Rubino, H.; Garner, C.; Shafit-Zagardo, B. : Genomic structure of human microtubule-associated protein 2 (MAP-2) and characterization of additional MAP-2 isoforms. Proc. Nat. Acad. Sci. 92: 10894-10898, 1995.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MAP-2 antibody

of primary neuronal culture showing neuronal staining with MAP2 (green; Catalog # CH22103) and astrocytes with GFAP (red; Catalog # MO19063). Nuclei are counterstained blue. Chicken Serum Hu, Ms, Prim, Rt C, ICC/IF, WB
50 µl / €390.00
  Neuromics Antibodies

MAP-2 antibody

  Chicken Anti-Microtubule Associated Protein 2 (MAP2) Antibody  
Western blot of rat cortex lysate showing specific immunolabeling of the ~280k MAP2 protein. Chicken Ig Ig Fraction Bov, Hu, Ms, Rt C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAP-2 antibody

  Mouse Monoclonal MAP2 Antibody (5H11)  
Western Blot: MAP2 Antibody (5H11) - Western blot of whole rat brain lysate probed with anti-MAP2 antibody to MAP2. Note that the strong single band running at about 280 kDa corresponds to MAP2. Mouse IgG2 5H11 Purified Bov, Hu, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAP-2 antibody

  Anti-Map2 Rabbit Polyclonal Antibody  
Predicted band size: 200 kDa . Positive control: Human lymphoma tissue lysate. Recommended dilution: 1/200-1000. (Gel: %SDS-PAGE Lysate: 50 ug per lane Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 90 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAP-2 antibody

  Anti-Map2 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm,Nucleus. Positive control: Human brain and colon cancer tissue . Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using Map2 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAP-2 antibody

  Mouse monoclonal MAP2 Antibody (Ascites)  
MAP2 Antibody (Cat. #TA324336) western blot analysis in MCF-7 cell line lysates (15ug/lane).This demonstrates the MAP2 antibody detected the MAP2 protein (arrow).(1:1000) Mouse IgG1 159CT34.12.3.4 Ascites Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAP-2 antibody

  MAP2 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using MAP2 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAP-2 antibody

  Chicken Polyclonal MAP2 Antibody  
Western Blot: MAP2 Antibody [TA336617] - Western blot analysis of MAP2 expression in bovine brain extract using TA336617. Chicken IgY Purified Bov, Hu, Ms, Rt C, ICC/IF, WB
50 µl / €325.00
  OriGene Technologies, Inc.

MAP-2 antibody

  Mouse Monoclonal MAP2 Antibody (4H5)  
Western Blot: MAP2 Antibody (4H5) [TA337109] - Western blot of rat brain extract; note that the strong single band running at about 280kDa corresponds to MAP2. Mouse IgG1 4H5 Purified Bov, Hu, Ms ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAP-2 antibody

  Mouse Monoclonal MAP2 (N-terminus) Antibody  
Western blot detection of MAP2(N-terminus) in Mouse Brain tissue and Hela cell lysates using MAP2(N-terminus) mouse mAb (1:1000 diluted).Predicted band size: 202KDa.Observed band size: 300KDa. Mouse IgG2b 1D7-B9-C8-F6 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAP-2 antibody

  Rabbit Polyclonal Anti-MAP2 Antibody  
Western blot analysis of extract from C6 cells using MAP2 Antibod Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAP-2 antibody

  Rabbit Polyclonal Anti-MAP 2 Antibody  
Western blot analysis of MAP2 expression in mouse brain Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAP-2 antibody

Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with MAP2 antibody AM20513PU-N followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG1 5F9 Purified Hu, Ms, Rb, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

MAP-2 antibody

Paraffin-Embedded Section of Rat Brain Tissue stained with Monoclonal Anti-MAP2 antibody Mouse IgG1 MP-2 Purified Rt C, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

MAP-2 (C-term) antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with MAP2 Antibody (C-term) (AP11882PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MAP-2 antibody

Immunohistochemistry (IHC) analyzes of MAP-2 antibody (Cat.-No.: AP20610PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

MAP-2 antibody

Dissociated cell cultures of an e13 Mouse brain showing MAP-2 (green staining) of neurons. DAPI (blue staining) allows visualization of nuclei. Chicken Ig Aff - Purified Hu, Ms ICC/IF, P, WB
0.2 mg / €500.00
  Acris Antibodies GmbH

MAP-2 (2a + 2b) antibody

Formalin-fixed, paraffin-embedded human brain stained with Microtubule-Associated Protein 2a,b Ab (Cat. BM2472) using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of the neurons and dendrites. Mouse IgG1 AP20 Purified Bov, Chk, Hu, Ms, Rt C, ICC/IF, WB
0.25 mg / €420.00
  Acris Antibodies GmbH

MAP-2 antibody

MAP-2 Mouse IgG1 AP20 Biotin Bov, Chk, Hu, Ms, Quail, Rt, Xen C, ICC/IF, P, WB
0.25 mg / €390.00
  Acris Antibodies GmbH

MAP-2 antibody

MAP-2 Mouse IgG1 AP20 FITC Bov, Chk, Hu, Ms, Quail, Rt, Xen C
0.25 mg / €450.00
  Acris Antibodies GmbH

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