MAP kinase p38 alpha / MAPK14 antibody

Principal name

MAP kinase p38 alpha / MAPK14 antibody

Alternative names for MAP kinase p38 alpha / MAPK14 antibody

Mitogen-activated protein kinase 14, p38 alpha, MXI2, SAPK2A, CSBP, CSBP1, CSBP2, CSPB1, Cytokine suppressive anti-inflammatory drug-binding protein, MAX-interacting protein 2

SwissProt ID

P47811 (Mouse), P70618 (Rat), Q16539 (Human)

Gene ID

1432 (MAPK14), 26416 (Mapk14), 7914326 (P41765)

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), African clawed frog, GP (Guinea Pig), Hst (Hamster), Mky (Monkey), Por (Porcine), Rb (Rabbit), Sh (Sheep), Chk (Chicken), Xen (Xenopus), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Functional assay (FN), Dot blot (Dot), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of MAP kinase p38 alpha / MAPK14 antibody

The MAPK (mitogen activated protein kinase) comprises a family of ubiquitous praline-directed, proteinserine/ threonine kinases which signal transduction pathways that control intracellular events including acute responses to hormones and major developmental changes in organisms (1). This super family consists of stress activated protein kinases (SAPKs); extracellular signal-regulated kinases (ERKs); and p38 kinases, each of which forms a separate pathway (2). The kinase members that populate each pathway are sequentially activated by phosphorylation. Upon activation, p38 MAPK/SAPK2alpha translocates into the nucleus where it phosphorylates one or more nuclear substrates, effecting transcriptional changes and other cellular processes involved in cell growth, division, differentiation, inflammation, and death (3). Specifically p38 always acts as a pro-apoptotic factor with its activation leading to the release of cytochrome c from mitochondria and cleavage of caspase 3 and its downstream effector, PARP (4). p38 MAPK is activated by a variety of chemical stress inducers including hydrogen peroxide, heavy metals, anisomycin, sodium salicylate, LPS, and biological stress signals such as tumor necrosis factor, interleukin-1, ionizing and UV irradiation, hyperosmotic stress and chemotherapeutic drugs (5). As a result, p38 alpha has been widely validated as a target for inflammatory disease including rheumatoid arthritis, COPD and psoriasis (6) and has also been implicated in cancer, CNS and diabetes (7).

General readings

Beardmore VA, et al. (2005) Mol Cell Biol. 25(23):10454-64.Chen, Z, et al (2001) J. Biol. Chem. (United States). 276 (19): 16070-5

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MAP kinase p38 pThr180/pTyr182 antibody

Western blot analysis of C-6 glioma cells using phospho-Thr180/Tyr182 antibody in the absence and presence of anisomycin. Rabbit IgG Aff - Purified Hu, Ms, Rt Dot, WB
10 Blots / €460.00
  Acris Antibodies GmbH

MAP kinase p38 pTyr182 antibody

Immunohistochemistry analysis of p-p38 Antibody (Cat.-No AP20851PU-N) in paraffin-embedded human colon carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

MAP kinase p38 alpha / MAPK14 antibody

Immunoperoxidase of monoclonal antibody to MAPK14 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 ug/ml] Mouse IgG1 3D5 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MAP kinase p38 alpha / MAPK14 antibody

MAPK14 Antibody (Y322) (Cat. #TA302203) western blot analysis in Jurkat cell line and mouse kidney tissue lysates (35ug/lane).This demonstrates the MAPK14 antibody detected the MAPK14 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAP kinase p38 alpha / MAPK14 antibody

Predicted band size: 43 kDa . Positive control: 293 and Hela cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1,2: 293 and Hela cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAP kinase p38 alpha / MAPK14 antibody

MAPK14 Antibody (Center T180/Y182) (Cat. #TA324492) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the MAPK14 antibody detected the MAPK14 protein (arrow). Rabbit IgG Purified Hu, Ms, Rt, Ze ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

MAP kinase p38 alpha / MAPK14 antibody

MAP kinase p38 alpha / MAPK14 Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAP kinase p38 alpha / MAPK14 antibody

Western blot analysis of extracts from 293/RAW246.7 cells, using p38 MAPK (Ab-182) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAP kinase p38 alpha / MAPK14 antibody

Western blot analysis of extracts from 293 cells, using p38 MAPK (Ab-322) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAP kinase p38 alpha / MAPK14 antibody

Western blot analysis of extracts from LOVO cells, HuvEc cells, K562 cells and HepG2 cells, using p38 MAPK antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms P
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAP kinase p38 alpha / MAPK14 antibody

Multi-blot analysis of p38Alpha MAPKinase in cell lysate from 12 human cancer cell lines using a 1:1000 dilution of the antibody Mouse IgG1 9F12 Purified Rt E, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAP kinase p38 alpha / MAPK14 antibody

Multi-blot analysis of p38 in a cell lysate from 12 human cancer cell lines using a 1:1000 dilution of the antibody Rabbit IgG Serum Bov, Can, Chk, GP, Hst, Hu, Mky, Ms, Por, Rb, Rt, Sh C, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAP kinase p38 alpha / MAPK14 antibody

Western blot analysis of extracts of various cell lines, using MAPK14 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAP kinase p38 alpha / MAPK14 (C-term) antibody

Anti-p38-α (DB 161): Western blot analysis of p38-α. Lane A – human recombinant p38-α (260-360a.a., N-terminal GST tag; Novus Biologicals, cat. #: H00001432-Q01), lane B – human recombinant p38-α (with C-terminal DDK tag, Novus Biologicals, cat. #: NBL1-12870), lane C - human recombinant PKC-β (1-368a.a., N-terminal GST tag, Novus Biologicals, cat. #: H00006300-P01), lane D – human recombinant p38-δ (1-366a.a., N-terminal GST tag, Novus Biologicals, cat. #: H00005603-P01). 500ng of recombinant protein was loaded in each well. Rabbit IgG E17-S Aff - Purified Hu, Ms, Rt E, ICC/IF, IP, WB
0.1 ml / €510.00
  DB Biotech, spol. s r.o.

Sample sizes available

MAP kinase p38 alpha / MAPK14 (C-term) antibody

Anti-p38-α (DB 161): Western blot analysis of p38-α. Lane A – human recombinant p38-α (260-360a.a., N-terminal GST tag; Novus Biologicals, cat. #: H00001432-Q01), lane B – human recombinant p38-α (with C-terminal DDK tag, Novus Biologicals, cat. #: NBL1-12870), lane C - human recombinant PKC-β (1-368a.a., N-terminal GST tag, Novus Biologicals, cat. #: H00006300-P01), lane D – human recombinant p38-δ (1-366a.a., N-terminal GST tag, Novus Biologicals, cat. #: H00005603-P01). 500ng of recombinant protein was loaded in each well. Rabbit IgG E17-S Aff - Purified Hu, Ms, Rt E, ICC/IF, IP, WB
50 µl / €340.00
  DB Biotech, spol. s r.o.

Sample sizes available

MAP kinase p38 alpha / MAPK14 antibody

MAP kinase p38 alpha / MAPK14 Rabbit IgG I15-E FITC Hu F
100 Tests / €560.00
  DB Biotech, spol. s r.o.

Sample sizes available

MAP kinase p38 alpha / MAPK14 (N-term) (incl. pos. control) antibody

Detection of endogenous Mxi / SAPK2alpha Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab Mxi-2F2 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Mab Mxi-2F2 recognizes SAPK2α/p38 at 38 kDa and its splice variant Mxi2 at 30 kDa. lane 1: A431; lane 2:SW480; lane 3: SW620; lane 4: HT29; lane 5: MCF7; lane 6: MDA231; lane 7: T47D Mouse IgG1 2F2 Purified Can, Hu, Ms, Rt E, IP, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

MAP kinase p38 alpha / MAPK14 (N-term) (incl. pos. control) antibody

Detection of endogenous SAPK2alpha Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab SAPK2a-13D5 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2:SW480; lane 3: SW620; lane 4: HT29; lane 5: MCF7; lane 6: MDA-MB231; lane 7: T47D Mouse IgG1 13D5 Purified Can, Hu, Ms, Rt E, ICC/IF, IP, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

MAP kinase p38 alpha / MAPK14 (N-term) (incl. pos. control) antibody

Detection of endogenous SAPK2alpha Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab SAPK2a-20B11 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2:SW480; lane 3: SW620; lane 4: HT29; lane 5: MCF7; lane 6: MDA-MB231; lane 7: T47D Mouse IgG1 20B11 Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

MAP kinase p38 alpha / MAPK14 antibody

Multi-blot analysis of p38Alpha MAPKinase in cell lysate from 12 human cancer cell lines using a 1:1000 dilution of the antibody Mouse IgG1 9F12 Purified Rt E, IP, WB
25 µg / €200.00
  Acris Antibodies GmbH

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