MAP2K1 antibody

Principal name

MAP2K1 antibody

Alternative names for MAP2K1 antibody

MAPKK 1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK1, PRKMK1, MAP kinase kinase 1, Dual specificity mitogen-activated protein kinase kinase 1

SwissProt ID

P31938 (Mouse), Q01986 (Rat), Q02750 (Human)

Gene ID

5604 (MAP2K1), 26395 (Map2k1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Bov (Bovine), Chk (Chicken), Mky (Monkey), Xen (Xenopus), Frog, Por (Porcine), Rb (Rabbit), Ze (Zebrafish), Hst (Hamster)

Available hosts

Rabbit, Mouse, Chicken

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Dot blot (Dot), Luminex (LMNX)

Background of MAP2K1 antibody

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.

General readings

1. Chong H, Vikis HG, Guan KL, Mechanisms of regulating the Raf kinase family. Cellular Signalling 15 (2003) 463-469.
2. Adams, J.P. and Sweatt, J.D., "Molecular psychology: Roles for the ERK MAP kinase cascade in memory." Annu. Rev. Pharmacol. Toxicol. 42 (2002) 135 - 163.
3. Mansour, S.J., Resing, K.A., Candi, J.M., Hermann, A.S., Gloor, J.W., Herskind, K.R., Wartmann, M., Davis, R.J., and Ahn, N.G., "Mitogen-activated protein (MAP) kinase phosphorylation of MAP kinase kinase: determination of phosphorylation sites by mass spectrometry and site-directed mutagenesis." J. Biochem. (Tokyo) 116 (1994) 304 - 14.
4. Ahn, N.G., "The MAP kinase cascade. Discovery of a new signal transduction pathway." Mol. Cell Biochem. 127 - 128 (1993) 201 - 209.
5. Kyriakis JM, Brautigan DL, Ingebritsen TS, Avruch J pp54 Microtubule-associated protein-2 kinase requires both tyrosine and serine/threonine phosphorylation for activity. J Biol Chem 266 (1991) 10043-10046.
6. Crews, C.M., Alessandrini, A., and Erikson, R.L. "The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product." Science 258 (1992) 478 - 480.
7. Seger R, Ahn NG, Boulton TG, Yancopoulos GD, Panayotatos N, Radziejewska E, Ericsson L, Bratlien RL, Cobb MH, Krebs EG Microtubule-associated protein 2 kinases, ERK1 and ERK2, undergo autophos-phorylation on both tyrosine and threonine residues: Implications for their mechanism of activation. Proc Natl Acad Sci USA 88 (1991) 6142-6146.
Quality Control Tests: Dot blots and Western blots performed with each lot.

  1. Quick-Filters:
  2. Cited (1)
  3. Highly Validated (40)

Items 1 to 20 of 173 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MAP2K1 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAP2K1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506006) Mouse IgG1 OTIE1 Purified Can, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAP2K1 antibody

Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-MAP2K1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500644, Dilution 1:50) Mouse IgG1 OTI1F5 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAP2K1 antibody

Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-MAP2K1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500672, Dilution 1:50) Mouse IgG2b OTI1C1 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAP2K1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MAP2K1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505702) Mouse IgG2a OTI2D4 Purified Can, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAP2K1 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MAP2K1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505703) Mouse IgG2b OTI2H3 Purified Can, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAP2K1 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAP2K1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505704) Mouse IgG2b OTI2G5 Purified Can, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAP2K1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MAP2K1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505705) Mouse IgG2a OTI1E5 Purified Can, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAP2K1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MAP2K1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505728) Mouse IgG2a OTI3B4 Purified Can, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAP2K1 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAP2K1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505729) Mouse IgG2a OTI2D2 Purified Can, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAP2K1 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MAP2K1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505742) Mouse IgG1 OTI2B7 Purified Can, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAP2K1 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MAP2K1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505745) Mouse IgG2a OTI2B2 Purified Can, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAP2K1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MAP2K1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505763) Mouse IgG2b OTI2A1 Purified Can, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAP2K1 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAP2K1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505764) Mouse IgG2b OTI2A9 Purified Can, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAP2K1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MAP2K1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505765) Mouse IgG2b OTI3H5 Purified Can, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAP2K1 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAP2K1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505766) Mouse IgG2b OTI4H8 Purified Can, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAP2K1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MAP2K1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505767) Mouse IgG2b OTI4F1 Purified Can, Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAP2K1 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAP2K1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505768) Mouse IgG1 OTI3H3 Purified Can, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAP2K1 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAP2K1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505770) Mouse IgG2b OTI4H1 Purified Can, Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAP2K1 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAP2K1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505774) Mouse IgG2a OTI4B1 Purified Can, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAP2K1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MAP2K1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505775) Mouse IgG2a OTI4E4 Purified Can, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Items 1 to 20 of 173 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5
  • LinkedIn