MAP2K5 antibody

Principal name

MAP2K5 antibody

Alternative names for MAP2K5 antibody

MAP kinase kinase 5, MAPKK 5, MEK5, MKK5, Dual specificity mitogen-activated protein kinase kinase 5

SwissProt ID

Q13163 (Human), Q62862 (Rat), Q9WVS7 (Mouse)

Gene ID

5607 (MAP2K5), 29568 (Map2k5), 23938

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Mky (Monkey), Xen (Xenopus), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Paraffin Sections (P), Frozen Sections (C), Dot blot (Dot), Immunoprecipitation (IP)

Background of MAP2K5 antibody

Mitogen-acitvated protein kinase kinase 5 (MKK5) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MAPKs require dual phosphorylation on threonine and tyrosine residues, an activation step carried out by MAPK kinase (1-2). The MAPK cascade plays an important role in signaling defense responses. Of the MKK family members, MKK5 exhibits the closest homology to MEK1 and MEK2, with 46% amino acid identity in the catalytic domain (3) MKK has been show to activate extracellular-signal-regulated protein kinase 5 (ERK5). Also, activated ERK5 phosphorylates mitogen- MKK5 extensively at Ser129, Ser137, Ser142 and Ser149, which are located within the region in MKK5 that is thought to interact with ERK5 (3). There have also been findings to that the MEK5 (MKK5)/ERK5 (BMK) pathway may play a role in prostate cancer (5).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MAP2K5 antibody

Immunofluorescence of monoclonal antibody to MAP2K5 on HeLa cell . [antibody concentration 10 ug/ml] Mouse IgG1 M1-E6 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MAP2K5 antibody

Predicted cell location: Cytoplasm. Positive control: Human colon and ovarian cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using MAP2K5 antibody at dilution 1/40, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAP2K5 (incl. pos. control) antibody

Detection of endogenous MKK5 Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab MKK5-14B5 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2 Mouse IgG1 14B5 Purified Can, Hu, Ms, Rt WB
0.1 mg / €430.00
  Acris Antibodies GmbH

MAP2K5 pSer311/pThr315 antibody

MAP2K5 Rabbit IgG Purified Bov, Can, Chk, Hu, Mky, Ms, Rt, Xen, Ze WB
0.1 ml / €510.00
  Acris Antibodies GmbH

MAP2K5 pSer311/pThr315 antibody

Figure 1. Western blot of rat testes lysate showing specific labeling of the ~49k MEK5 protein (Control).  Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown). Rabbit IgG Aff - Purified Bov, Can, Chk, Hu, Ms, non-Human Primates, Rt, Xen, Ze WB
0.1 ml / €380.00
  Acris Antibodies GmbH

MAP2K5 antibody

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with MAP2K5 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MAP2K5 antibody

MAP2K5 Mouse Serum Hu E
50 µl / €290.00
  Abnova Taiwan Corp.

MAP2K5 antibody

Immunofluorescence of purified MaxPab antibody to MAP2K5 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

MAP2K5 antibody

Immunoprecipitation of MAP2K5 transfected lysate using anti-MAP2K5 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with MAP2K5 MaxPab mouse polyclonal antibody (B01) (H00005607-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

MAP2K5 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between MAP3K3 and MAP2K5 HeLa cells were stained with anti-MAP3K3 rabbit purified polyclonal 1:1200 and anti-MAP2K5 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG1 1E5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MAP2K5 antibody

MAP2K5 Mouse IgG1 2C2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MAP2K5 antibody

MAP2K5 Mouse IgG1 1F6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MAP2K5 antibody

Detection limit for recombinant GST tagged MAP2K5 is approximately 10ng/ml as a capture antibody. Mouse IgG1 1B4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MAP2K5 antibody

Immunofluorescence of monoclonal antibody to MAP2K5 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG1 3E10 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MAP2K5 antibody

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with MAP2K5 polyclonal antibody ( Cat # PAB2270 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

MAP2K5 antibody

Western blot of rat testis lysate showing specific labeling of the ~49k Map2k5 protein (Control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown). Rabbit Bov, Chk, Hu, Ms, Rt WB
0.1 ml / €510.00
  Abnova Taiwan Corp.

MAP2K5 antibody

The whole cell lysates derived from EGF-stimulated A-431 were immunoblotted by MAP2K5 ( phospho S311/T315 ) polyclonal antibody ( Cat # PAB12634 ) at 1 : 1000 ( lane 1 ). The lane 2 was pre-absorbed by immunizing peptide. Rabbit Chk, Hu, Ms, Rt C, Dot, E, IP, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

MAP2K5 antibody

The tissue lysates derived from mouse liver were immunoblotted by MAP2K5 polyclonal antibody ( Cat # PAB12704 ) at 1 : 1000. Rabbit Chk, Hu, Ms, Rt C, E, IP, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

MAP2K5 antibody

The tissue lysates derived from mouse liver were immunoblotted by MAP2K5 polyclonal antibody ( Cat # PAB12705 ) at 1 : 1000 ( lane 1 ). The lane 2 was a negative control. Rabbit Chk, Hu, Ms, Rt C, Dot, E, IP, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

MAP2K5 (N-term) antibody

Mouse Heart; WB Suggested Anti-Map2k5 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Heart; Map2k5 antibody - N-terminal region (ARP49021_P050) in Mouse Heart cells using Western Blot Rabbit Aff - Purified Hu, Ms WB
50 µg / €350.00
  AVIVA Systems Biology

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