MAP2K6 antibody

Principal name

MAP2K6 antibody

Alternative names for MAP2K6 antibody

MAPKK 6, MAPK/ERK kinase 6, SAPKK3, MEK6, MKK6, MAP kinase kinase 6, Dual specificity mitogen-activated protein kinase kinase 6

SwissProt ID

P52564 (Human), P70236 (Mouse), Q5E9X2 (Bovin)

Gene ID

5608 (MAP2K6), 26399 (Map2k6)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of MAP2K6 antibody

MAP2K6 (Mitogen-activated protein kinase kinase 6), also known as MAP kinase kinase 6 (MAPKK 6) or MAPK/ERK kinase 6 is an enzyme that in humans is encoded by the MAP2K6 gene, on chromosome 17. MAPKK 6 is a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. Han et al. (1996) described experiments addressing the function of MKK6 in intracellular signaling pathways leading toward activation of p38 MAPK. Mukherjee et al. (2006) found that the Yersinia YopJ virulence factor acted as an acetyltransferase, using coenzyme A to modify critical ser and thr residues in the MAP2K6 activation loop, thereby blocking phosphorylation and kinase activation. They proposed that ser and/or thr acetylation by a bacterial effector may be a mechanism that occurs in other signaling pathways.

General readings

Manning G., et al. (2002) Science 298 (5600): 1912–34Pearson G., et al. (2002) Endocr. Rev. 22 (2): 153–83

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MAP2K6 antibody

Immunofluorescence analysis of Hela cells using MAP2K6 antibody Cat.-No AM06577SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Mouse IgG1 3H12C8 Ascites Hu, Ms E, ICC/IF, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

MAP2K6 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between MAP2K3 and MAP2K6 HeLa cells were stained with anti-MAP2K3 rabbit purified polyclonal 1:1200 and anti-MAP2K6 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2a 2F2 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MAP2K6 antibody

Western blot analysis of extracts from MDA-MB-435 cells untreated or treated with UV, using MKK antibody (Lanes 1 and 2) and MKK (phospho-Ser207) antibody (Lanes 3 and 4). Rabbit IgG Purified Hu, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAP2K6 antibody

Western blot analysis of extracts from MDA-MB-435 cells, using MKK3 (Ab-189) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAP2K6 antibody

Western blot analysis of extracts from 293 cells, using MAP2K6 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAP2K6 antibody

Predicted band size: 37 kDa . Positive control: Mouse muscle tissue lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lysate: 30 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAP2K6 antibody

Predicted cell location: Cytoplasm. Positive control: Human lung and esophagus cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human lung and esophagus cancer tissue using MAP2K6 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAP2K6 antibody

Western blot analysis of extracts from MDA-MB-435 cells, using MKK6 (Ab-207) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAP2K6 antibody

Western blot analysis of extracts of HeLa cell line, using MAP2K6 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAP2K6 antibody

Western blot analysis of extracts from HL60 cells, using phospho-MAP2K6-S207 antibody Rabbit IgG Purified Hu, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAP2K6 antibody

Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-MKK6 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Poly6047 Purified Hu, Ms WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

MAP2K6 antibody

Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-MKK6 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit Poly6047 Purified Hu, Ms WB
50 µl / €248.00
  OriGene Technologies, Inc.

MAP2K6 antibody

Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and kidney cancer (right) using MAP2K6 antibody Cat.-No AM06578SU-N with DAB staining. Mouse IgG1 3H12B9 Ascites Hu, Ms E, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

MAP2K6 pSer207 antibody

MAP2K6 Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

MAP2K6 pSer207 antibody

Figure 1. Immunohistochemical analysis of Paraffin-Embedded Human breast carcinoma tissue, using MKK6 (phospho-Ser207) antibody (AP02431PU) and the same antibody preincubated blocking peptide. Rabbit Aff - Purified Hu, Rt ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

MAP2K6 pSer207 antibody

Figure 1. Immunohistochemical analysis of Paraffin-Embedded Human breast carcinoma tissue, using MKK6 (phospho-Ser207) antibody (AP02431PU) and the same antibody preincubated blocking peptide. Rabbit Aff - Purified Hu, Rt ICC/IF, P, WB
50 µl / €240.00
  Acris Antibodies GmbH

MAP2K6 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using MKK6 antibody AP02676PU. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

MAP2K6 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using MKK6 antibody AP02676PU. Rabbit Aff - Purified Hu ICC/IF, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

MAP2K6 antibody

Immunohistochemistry (IHC) analyzes of MKK6 antibody (Cat.-No.: AP06223PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

MAP2K6 antibody

Western blot (WB) analysis of MEK-6 antibody (Cat.-No.: AP06705PU-N) in extracts from 293 cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

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