MAP3K12 antibody

Principal name

MAP3K12 antibody

Alternative names for MAP3K12 antibody

Mixed lineage kinase, MAPK-upstream kinase, Mitogen-activated protein kinase kinase kinase 12, Leucine-zipper protein kinase, Dual leucine zipper bearing kinase

SwissProt ID

Q12852 (Human), Q60700 (Mouse), Q63796 (Rat)

Gene ID

7786 (MAP3K12), 6264040 (IPR001957), 26404 (Map3k12)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of MAP3K12 antibody

Mitogen-activated protein kinase kinase kinase 12 (MAP3K12) is a MAP kinase kinase kinase enzyme belonging to the serine/threonine protein kinase family (1). Located on human chromosome 12q13, it regulates the activity of various MAPKKs such as MAPKK7 and hence activating C-Jun N-terminal Kinase (JNK/SAPK). MAP3K12 associates with scaffold proteins, JIPs, which also interacts with MAPKK7 and JNK/SAPK (2). MAP3K12 protein is associated with dotted structures that are regularly located with golgi apparatus and also along the microtubules (3). This protein contains two leucine-zipper-like motifs and interaction of MAP3K12-binding inhibitory protein with one of these motifs deactivates this protein and induces activation of SAPK/JNK (4). MAP3K12 is also implicated in regulating radial cell migration via microtubule-based events. It has been reported that ectopic expression of this protein in neuronal precursor cells allows these cells to migrate from the ventricular zone and differentiate into neural cells (3). MAP3K12 was identified as a signaling molecule that is required for the regulation of keratinocyte terminal differentiation and cornification (5). While MAP3K12 is found in the epidermis, it is highly restricted to the neuronal cells in the central, peripheral and autonomic nervous systems. More specifically, it is localized in the axons of these cells (6). It has been shown that over-expression of MAP3K12 in cos-1 cells impairs the radial organization of microtubules without massive depolymerization. In humans, MAP3K12 is approximately a 68kDa protein (567 amino acids). In rat and mouse this protein weighs 107kDa (888 amino acids). The MAP3K12 antibodies were generated using peptide corresponding to the human MAP3K12 protein. MAP3K12 antibodies are affinity purified over immobilized antigen based affinity chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer.

11 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MAP3K12 antibody

Predicted cell location: Cytoplasm. Positive control: Human colon and thyroid cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using MAP3K12 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAP3K12 antibody

Predicted cell location: Cytoplasm. Positive control: Human cervical and liver cancer tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using MAP3K12 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAP3K12 antibody

Immunoblot against extracts of COS cells transiently transfected with GFP-tagged MAP3K12, MAP3K13 or untagged Kv2.1 plasmid; adult rat brain low-speed pellet (RBLSP) or membrane (RBM) fractions; and membranes from MAP3K12 wild-type (WT) and genetrap knockout (KO) mice probed with N377/20 (left) or N52A/42 (right) TC supe. Mouse brains courtesy of Aki Itoh and Takayuki Itoh (UC Davis). Mouse IgG2a N377/20 Supernatant Ms, Rt C, ICC/IF, WB
5 ml / €320.00
  Antibodies Incorporated

NeuroMab

MAP3K12 antibody

Immunoblot against extracts of COS cells transiently transfected with GFP-tagged MAP3K12, MAP3K13 or untagged Kv2.1 plasmid; adult rat brain low-speed pellet (RBLSP) or membrane (RBM) fractions; and membranes from MAP3K12 wild-type (WT) and gene-trap knockout (KO) mice probed with N377/83 (left) or N52A/42 (right) TC supe. Mouse brains courtesy of Aki Itoh and Takayuki Itoh (UC Davis). Mouse IgG1 N377/83 Supernatant Ms, Rt C, ICC/IF, WB
5 ml / €320.00
  Antibodies Incorporated

NeuroMab

MAP3K12 antibody

Immunoblot against extracts of COS cells transiently transfected with GFP-tagged MAP3K12, MAP3K13 or untagged Kv2.1 plasmid; adult rat brain low-speed pellet (RBLSP) or membrane (RBM) fractions; and membranes from MAP3K12 wild-type (WT) and genetrap knockout (KO) mice probed with N377/20 (left) or N52A/42 (right) TC supe. Mouse brains courtesy of Aki Itoh and Takayuki Itoh (UC Davis). Mouse IgG2a N377/20 Purified Ms, Rt C, ICC/IF, WB
0.1 ml / €380.00
  Antibodies Incorporated

NeuroMab

MAP3K12 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue (breast carcinoma) reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MAP3K12 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with MAP3K12 polyclonal antibody ( Cat # PAB2777 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu, Ms E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

MAP3K12 antibody

MAP3K12 Rabbit IgG Aff - Purified Hu E, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

MAP3K12 antibody

Immunohistochemistry-Paraffin: MAP3K12 Antibody [NBP1-85013] - Immunohistochemical staining of human stomach, upper shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MAP3K12

MAP3K12 Human
5 µg / €610.00
  Abnova Taiwan Corp.

MAP3K12

MAP3K12 Human Purified
0.25 mg / €280.00
  Novus Biologicals Inc.

11 Item(s)

per page
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