MAP3K3 antibody

Principal name

MAP3K3 antibody

Alternative names for MAP3K3 antibody

MAPKKK3, MEKK3, MEK kinase 3, MAPK/ERK kinase kinase 3, Mitogen-activated protein kinase kinase kinase 3

SwissProt ID

Q61084 (Mouse), Q99759 (Human)

Gene ID

4215 (MAP3K3), 26406 (Map3k3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of MAP3K3 antibody

MAP3K3(Mitogen-activated protein kinase kinase kinase 3), also known as MAP/ERK KINASE KINASE 3, MEKK3 or MAPKKK3, is an enzyme that in humans is encoded by the MAP3K3 gene. MAP3K3 is contiguously distal to LYK5 on chromosome 17 (Puffenberger et al., 2007). By yeast 2-hybrid analysis of a mouse T-cell cDNA library, Uhlik et al. (2003) showed that a C-terminal fragment of mouse Osm (CCM2) interacted with Mekk3, which activates p38 in response to sorbitol-induced hyperosmotic conditions. Mekk3 and Osm colocalized in the cytoplasmic compartment of cotransfected cells, and the Mekk3-Osm complex was recruited to Rac1 and cytoskeletal actin-containing membrane ruffles in response to sorbitol treatment. Protein interaction assays showed that Osm interacted directly with the Mekk3 substrate Mkk3 (MAP2K3), with actin, and with both GDP- and GTP-loaded Rac1. Uhlik et al. (2003) concluded that the RAC1-OSM-MEKK3-MKK3 complex is required for regulation of p38 activity in response to osmotic shock.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MAP3K3 antibody

Western blot analysis of extracts from HepG2 cells, using MAP3K3 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAP3K3 antibody

Western blot analysis on HepG2 cell lysate using MAP3K3 Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAP3K3 antibody

Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Serum Ms P, WB
50 µl / €470.00
  Acris Antibodies GmbH

MAP3K3 antibody

Western Blot analysis of MAP3K3 expression in transfected 293T cell line (H00004215-T01) by MAP3K3 MaxPab polyclonal antibody.

Lane 1: MAP3K3 transfected lysate(10.01 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

MAP3K3 antibody

Western Blot analysis of MAP3K3 expression in transfected 293T cell line (H00004215-T02) by MAP3K3 MaxPab polyclonal antibody.

Lane 1: MAP3K3 transfected lysate(9.9 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

MAP3K3 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between MAP3K3 and MAP2K5 HeLa cells were stained with anti-MAP3K3 rabbit purified polyclonal 1:1200 and anti-MAP2K5 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

MAP3K3 antibody

MAP3K3 Mouse IgG2a 1H3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MAP3K3 antibody

Western blot analysis of extracts from HepG2 cells, using MAP3K3 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €205.00
  Assay Biotech

MAP3K3 antibody

Western blot analysis of extracts from HepG2 cells, using MAP3K3 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €310.00
  Assay Biotech

MAP3K3 antibody

MAP3K3 Rabbit IgG Aff - Purified Hu WB
0.1 mg / €290.00
  Boster Immunoleader

MAP3K3 antibody

MAP3K3 Rabbit IgG Aff - Purified Ms, Rt P, WB
0.1 mg / €290.00
  Boster Immunoleader

MAP3K3 antibody

Immunohistochemical analysis of MEKK3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

MAP3K3 antibody

Immunohistochemical analysis of MEKK3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

MAP3K3 antibody

Immunohistochemical analysis of MEKK3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

CRISPR

Catalog No. Species Donor Vector  

MAP3K3 - human gene knockout kit via CRISPR

MAP3K3 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

MAP3K3 - human gene knockout kit via CRISPR

MAP3K3 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

MAP3K3 - human gene knockout kit via CRISPR

MAP3K3 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

MAP3K3 - human gene knockout kit via CRISPR

MAP3K3 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MAP3K3 (full length, N-term HIS tag, transcript variant 2)

MAP3K3 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

MAP3K3

MAP3K3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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