MAP3K8 / TPL-2 antibody

Principal name

MAP3K8 / TPL-2 antibody

Alternative names for MAP3K8 / TPL-2 antibody

MAPK, Tumor progression locus 2, COT, ESTF, COT proto-oncogene serine/threonine-protein kinase, Cancer Osaka thyroid oncogene, Tumor progression locus 2, Mitogen-activated protein kinase kinase kinase 8

SwissProt ID

P41279 (Human), Q07174 (Mouse), Q63562 (Rat)

Gene ID

1326 (MAP3K8)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Eq (Equine), Hst (Hamster)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Enzyme Immunoassay (E), Dot blot (Dot), Immunoprecipitation (IP)

Background of MAP3K8 / TPL-2 antibody

Tumor progression locus 2 (Tpl2) is a hematopoietically expressed serine-threonine protein kinase critical in innate immunity linking TOLL receptor (TLRs) to TNF production via activation of its extracellular signal regulated kinase (ERK) kinase (1). MAP3K8 is shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This kinase was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. Gram positive bacterial infection like Listeria monocytogenes is capable of activating both TLRs and non TLR particle recognition receptors (PRRs) and cause MAP3K8 dependent production of TNF, IFN and IL2 (2). MAP3K8 activation also promotes Androgen depletion-independent prostate cancer growth (3). MAP3K8 is selectively involved in inflammatory cytokine-induced ERK1/2 activation in adipocytes and is implicated in dysfunction of adipose tissue in obesity (4). MAP3K8 was identified as a signaling molecule that is required for the regulation of critical function of T helper cells in promoting expression of transcription factors that derived optimal Th1 differentiation (5). MAP3K8 is expressed in immune cells whilst, over expression of MAP3K8 in PBMC causes dys-regulation og IFN-Y, STS4, IL-12 and many other cytokines (6). MAP3K8 gene is located on chromosome 10p11.23, in humans; MAP3K8 is approximately a 57Da protein (467 amino acids). In rat and mouse this protein has similar molecular weights with some conservative substitutions. MAP3K8 gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. The MAP3K8 antibodies were generated using peptide corresponding to the human MAP3K8 protein. MAP3K8 antibodies are affinity purified over immobilized antigen based affinity chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer. FabGennix Int. Inc. will provide limited quantities of antigenic blocking protein for competition assays involving MAP3K8 antibodies. Antibodies to MAP3K8 and will label ~57kDa protein in Western blot positive control for MAP3K8 and several other tissues.

General readings

1. Jeong JH  et al. TPL2/COT/MAP3K8 (TPL2) Activation Promotes Androgen Depletion-Independent (ADI) Prostate Cancer Growth. PLoS One 6:e16205 (2011). WB ; Human . PubMed: 21267413

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MAP3K8 / TPL-2 antibody

Western blot analysis of extracts from COLO cells, treated with Insulin (0.01U/ml, 15mins), using MAP3K8 (Ab-400) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAP3K8 / TPL-2 antibody

Western blot analysis of extracts from 293 cells treated with LPS (100ng/ml, 30min), using COT (Ab-290) antibody (#TA312191, Line 1 and 2).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAP3K8 / TPL-2 antibody

Western blot analysis of COT expression in LPS treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAP3K8 / TPL-2 antibody

Anti-MAP3K8 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Eq, Hst, Hu, Ms, Rt
50 µg / €379.00
  OriGene Technologies, Inc.

MAP3K8 / TPL-2 antibody

Anti-MAP3K8 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Can, Hst, Hu
50 µg / €379.00
  OriGene Technologies, Inc.

MAP3K8 / TPL-2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP3K8 (RC211171, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP3K8.(1:500) Mouse IgG1 OTI3D9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAP3K8 / TPL-2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP3K8 (RC211171, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP3K8.(1:2000) Mouse IgG1 OTI2E10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAP3K8 / TPL-2 antibody

Immunohistochemical (IHC) analysis in paraffin-embedded human brain tissue using MAP3K8 / TPL-2 antibody (Cat.-No AP06071PU-N). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

MAP3K8 / TPL-2 pThr290 antibody

Dot blot analysis of anti-MAP3K8 pThr290 Phospho-specific Pab (Cat.#AP12835PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €370.00
  Acris Antibodies GmbH

MAP3K8 / TPL-2 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MAP3K8 / TPL-2 (C-term) antibody

(LEFT)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.  (RIGHT)Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with COT Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MAP3K8 / TPL-2 pSer400 antibody

Immunohistochemical analysis of paraffin-embedded human brain tissue using MAP3K8 (Phospho-Ser400) antibody AP55762PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms, Rt P
0.1 ml / €340.00
  Acris Antibodies GmbH

MAP3K8 / TPL-2 pSer400 antibody

Immunohistochemical analysis of paraffin-embedded human brain tissue using MAP3K8 (Phospho-Ser400) antibody AP55762PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms, Rt P
50 µl / €270.00
  Acris Antibodies GmbH

MAP3K8 / TPL-2 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the MAP3K8 polyclonal antibody ( Cat # PAB2282 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu E, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

MAP3K8 / TPL-2 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with MAP3K8 polyclonal antibody ( Cat # PAB3169 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

MAP3K8 / TPL-2 antibody

Dot blot analysis of MAP3K8 (phospho T290) polyclonal antibody ( Cat # PAB8091 ) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg/ml. Rabbit Hu Dot, E
0.4 ml / €400.00
  Abnova Taiwan Corp.

MAP3K8 / TPL-2 pThr290 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using COT (Phospho-Thr290) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

MAP3K8 / TPL-2 pThr290 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using COT (Phospho-Thr290) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

MAP3K8 / TPL-2 pSer400 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using MAP3K8 (Phospho-Ser400) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
50 µg / €205.00
  Assay Biotech

MAP3K8 / TPL-2 pSer400 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using MAP3K8 (Phospho-Ser400) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
0.1 mg / €310.00
  Assay Biotech

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