MAP3K8 / TPL-2 antibody

Principal name

MAP3K8 / TPL-2 antibody

Alternative names for MAP3K8 / TPL-2 antibody

MAPK, Tumor progression locus 2, COT, ESTF, COT proto-oncogene serine/threonine-protein kinase, Cancer Osaka thyroid oncogene, Tumor progression locus 2, Mitogen-activated protein kinase kinase kinase 8

SwissProt ID

P41279 (Human), Q07174 (Mouse), Q63562 (Rat)

Gene ID

1326 (MAP3K8), 26410 (Map3k8), 116596

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Eq (Equine), Hst (Hamster)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Enzyme Immunoassay (E), Dot blot (Dot)

Background of MAP3K8 / TPL-2 antibody

The role of mitogen-activated protein kinases (MAPKs) in cell signaling pathways is well established. The rat gene Tpl-2, for tumor progression locus 2, and the human and mouse homologues c-Cot, for cancer osaka thyroid oncogene, encode a proto-oncogene serine/threonine protein kinase that was shown to play a role in the functional activation of the MAP kinase pathway. Overexpression of Cot induces MAP kinase activation in COS-1 and NIH/3T3 cells. Cot-mediated activation of MAP kinase is inhibited by both Ras N17, a dominant negative mutant of c-H-Ras, and Raf-1s621A, a dominant negative mutant of Raf-1, suggesting that Cot functions upstream of Ras and Raf-1.Other studies have shown that a kinase-negative, dominant negative mutant of Cot partially blocks Ras or Raf-1-induced MAP kinase activation, arguing that Cot functions downstream of Ras and Raf-1. To explain these contrasting findings, it has been suggested that Cot, Ras and Raf-1 may form a multimeric complex that phosphorylates MEK-1. Cot has also been shown to be implicated in T lymphocyte activation.

General readings

1. Jeong JH  et al. TPL2/COT/MAP3K8 (TPL2) Activation Promotes Androgen Depletion-Independent (ADI) Prostate Cancer Growth. PLoS One 6:e16205 (2011). WB ; Human . PubMed: 21267413

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MAP3K8 / TPL-2 antibody

  Rabbit polyclonal MAP3K8 (Ab-400) antibody  
Western blot analysis of extracts from COLO cells, treated with Insulin (0.01U/ml, 15mins), using MAP3K8 (Ab-400) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAP3K8 / TPL-2 antibody

  Rabbit polyclonal COT (Ab-290) antibody  
Western blot analysis of extracts from 293 cells treated with LPS (100ng/ml, 30min), using COT (Ab-290) antibody (#TA312191, Line 1 and 2).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAP3K8 / TPL-2 antibody

  Rabbit Polyclonal COT Antibody  
Western blot analysis of COT expression in LPS treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAP3K8 / TPL-2 antibody

  Rabbit Polyclonal COT (Thr290) Antibody (Phospho-specific)  
Western blot analysis of COT phosphorylation expression in UV treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAP3K8 / TPL-2 antibody

  Rabbit Polyclonal anti-MAP3K8 antibody  
MAP3K8 / TPL-2 Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAP3K8 / TPL-2 antibody

  Rabbit Polyclonal anti-MAP3K8 antibody  
MAP3K8 / TPL-2 Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

MAP3K8 / TPL-2 antibody

  Rabbit Polyclonal Anti-MAP3K8 Antibody (C-Terminus)  
Anti-MAP3K8 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Eq, Hst, Hu, Ms, Rt
50 µg / €379.00
  OriGene Technologies, Inc.

MAP3K8 / TPL-2 antibody

  Rabbit Polyclonal Anti-MAP3K8 Antibody (N-Terminus)  
Anti-MAP3K8 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Can, Hst, Hu
50 µg / €379.00
  OriGene Technologies, Inc.

MAP3K8 / TPL-2 antibody

  MAP3K8 mouse monoclonal antibody, clone OTI3D9 (formerly 3D9)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP3K8 (RC211171, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP3K8.(1:500) Mouse IgG1 OTI3D9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAP3K8 / TPL-2 antibody

  MAP3K8 mouse monoclonal antibody, clone OTI2E10 (formerly 2E10)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP3K8 (RC211171, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP3K8.(1:2000) Mouse IgG1 OTI2E10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAP3K8 / TPL-2 antibody

Immunohistochemical (IHC) analysis in paraffin-embedded human brain tissue using MAP3K8 / TPL-2 antibody (Cat.-No AP06071PU-N). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

MAP3K8 / TPL-2 pThr290 antibody

Dot blot analysis of anti-MAP3K8 pThr290 Phospho-specific Pab (Cat.#AP12835PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €370.00
  Acris Antibodies GmbH

MAP3K8 / TPL-2 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MAP3K8 / TPL-2 (C-term) antibody

(LEFT)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.  (RIGHT)Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with COT Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MAP3K8 / TPL-2 pSer400 antibody

Immunohistochemical analysis of paraffin-embedded human brain tissue using MAP3K8 (Phospho-Ser400) antibody AP55762PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms, Rt P
0.1 ml / €340.00
  Acris Antibodies GmbH

MAP3K8 / TPL-2 pSer400 antibody

Immunohistochemical analysis of paraffin-embedded human brain tissue using MAP3K8 (Phospho-Ser400) antibody AP55762PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms, Rt P
50 µl / €270.00
  Acris Antibodies GmbH

MAP3K8 / TPL-2 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the MAP3K8 polyclonal antibody ( Cat # PAB2282 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu E, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

MAP3K8 / TPL-2 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with MAP3K8 polyclonal antibody ( Cat # PAB3169 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

MAP3K8 / TPL-2 antibody

Dot blot analysis of MAP3K8 (phospho T290) polyclonal antibody ( Cat # PAB8091 ) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg/ml. Rabbit Hu Dot, E
0.4 ml / €400.00
  Abnova Taiwan Corp.

MAP3K8 / TPL-2 pThr290 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using COT (Phospho-Thr290) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €230.00
  Assay Biotech

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