MAPK10 antibody

Principal name

MAPK10 antibody

Alternative names for MAPK10 antibody

Stress-activated protein kinase JNK3, c-Jun N-terminal kinase 3, JNK3A, PRKM10, MAP kinase p49 3F12, Mitogen-activated protein kinase 10

SwissProt ID

P49187 (Rat), P53779 (Human), Q61831 (Mouse)

Gene ID

5602 (MAPK10)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of MAPK10 antibody

The stress-activated protein kise/Jun-amino-termil kise SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, growth factors and GPCR agonists. As with the other MAPKs, the core sigling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kises (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kise . Stress sigls are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs. Altertively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germil center kise (GCK) family member. There are three SAPK/JNK genes each of which undergoes altertive splicing, resulting in numerous isoforms. SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MAPK10 antibody

  Rabbit polyclonal anti-MAPK10 antibody  
Western blot analysis of extracts from HeLa cells, 293 cells and Jurkat cells, using MAPK10 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAPK10 antibody

  Anti-MAPK10 Rabbit Polyclonal Antibody  
Predicted band size: 53 kDa . Positive control: 293T cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAPK10 antibody

  MAPK10 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using MAPK10 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAPK10 antibody

  Rabbit Polyclonal Anti-MAPK10 Antibody - N-terminal region  
WB Suggested Anti-Mapk10 Antibody; Titration: 1.0 ug/ml; Positive Control: Rat Liver Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

MAPK10 (incl. pos. control) antibody

Detection of endogenous SAPK1 beta
Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab SAPK1b/jnk3-4G6 (0.5 μg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5;
lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2 Mouse IgG 4G6 Purified Hu WB
0.1 mg / €430.00
  Acris Antibodies GmbH

MAPK10 antibody

Immunofluorescence staining of methanol-fixed A431 (left) and Hela (right) cells showing cytoplasmic and membrane localization. Mouse IgG1 10E4A4 Ascites Hu, Ms ICC/IF, WB
0.1 ml / €460.00
  Acris Antibodies GmbH

MAPK10 (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue (breast carcinoma) reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MAPK10 (N-term) antibody

Formalin-fixed, Paraffin-embedded human cancer tissue stained with MAPK10 Antibody (N-term) Cat.-No AP14123PU-N which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MAPK10 antibody

MAPK10 Mouse IgG1 4E12-1F7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MAPK10 antibody

MAPK10 monoclonal antibody (M03), clone 3A3-1A8 Western Blot analysis of MAPK10 expression in A-431 ( Cat # L015V1 ). Mouse IgG1 3A3-1A8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MAPK10 antibody

MAPK10 monoclonal antibody (M05), clone 3B9 Western Blot analysis of MAPK10 expression in HeLa ( Cat # L013V1 ). Mouse IgG1 3B9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MAPK10 antibody

MAPK10 Mouse IgG1 2B2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MAPK10 antibody

Western blot analysis using MAPK10 monoclonal antibody, clone 2G12H3 ( Cat # MAB5533 ) ( isoform type 4 ) against recombinant MAPK10 ( Lane 1 ) and NIH/3T3 ( Lane 2 ). Mouse IgG1 2G12H3 Hu E, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

MAPK10 antibody

Immunohistochemical analysis of paraffin-embedded human brain tissue and lung carcinoma tissue, showing nuclear/cytoplasmic localization using MAPK10 monoclonal antibody, clone 8A5D11 ( Cat # MAB5534 ) with DAB staining. Mouse IgG1 8A5D11 Hu E, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

MAPK10 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with MAPK10 polyclonal antibody ( Cat # PAB2784 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu, Ms E, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

MAPK10 antibody

MAPK10 Rabbit Hu, Ms C, E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

MAPK10 antibody

Western blot analysis of extracts from HeLa/293/Jurkat cells, using MAPK10 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €230.00
  Assay Biotech

MAPK10 antibody

Western blot analysis of extracts from HeLa/293/Jurkat cells, using MAPK10 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €330.00
  Assay Biotech

MAPK10 antibody

Immunofluorescent analysis of JNK3 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, IP, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

MAPK10 antibody

Immunofluorescent analysis of JNK3 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, IP, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

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