MAPK12 antibody

Principal name

MAPK12 antibody

Alternative names for MAPK12 antibody

Mitogen-activated protein kinase 12, ERK5, Stress-activated protein kinase 3, p38 gamma, ERK6, SAPK3, MAP kinase p38 gamma, Extracellular signal-regulated kinase 6

SwissProt ID

O08911 (Mouse), P53778 (Human), Q63538 (Rat)

Gene ID

6300 (MAPK12), 29857 (Mapk12)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Mky (Monkey), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Flow Cytometry (F)

Background of MAPK12 antibody

MAP (mitogen-activated protein) kinases play a significant role in many biological processes, including cell adhesion and spreading, cell differentiation and apoptosis. p38 alpha, p38 beta and p38 gamma, also known as MAPK14, MAPK11 and MAPK12, respectively, each contain one protein kinase domain and belong to the MAP kinase family. Expressed in different areas throughout the body with common expression patterns in heart, p38 proteins use magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins. Via their catalytic activity, p38 alpha, p38 beta and p38 gamma are involved in a variety of events throughout the cell, including signal transduction pathways, cytokine production and cell proliferation and differentiation. The p38 proteins are subject to phosphoryation on Thr and Tyr residues, an event which is thought to activate the phosphorylated protein.

General readings

Han J., et al. (1995) Biochim Biophys Acta. 1265:224-227.Court N W., et al. (2002) J Mol Cell Cardiol. 34:413-426.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MAPK12 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAPK12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500500) Mouse IgG1 OTI10E1 Purified Hu F, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

MAPK12 antibody

WB Suggested Anti-MAPK12 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Por, Rb, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

MAPK12 antibody

WB Suggested Anti-Mapk12 Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Liver Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

MAPK12 (C-term) antibody

Anti-p38-ϒ (DB 163): Western blot analysis of p38-ϒ. Lane A – human recombinant p38-α (260-360a.a., N-terminal GST tag; Novus Biologicals, cat. #: H00001432-Q01), lane B – human recombinant p38-β (with C-terminal DDK tag, Novus Biologicals, cat. #: NBL1-12870), lane C - human recombinant PKC-ϒ (1-368a.a., N-terminal GST tag, Novus Biologicals, cat. #: H00006300-P01), lane D – human recombinant p38-δ (1-366a.a., N-terminal GST tag, Novus Biologicals, cat. #: H00005603-P01). 500ng of recombinant protein was loaded in each well. Rabbit IgG R13-L Aff - Purified Hu, Ms, Rt E, ICC/IF, IP, WB
0.1 ml / €510.00
  DB Biotech, spol. s r.o.

Sample sizes available

MAPK12 (C-term) antibody

Anti-p38-ϒ (DB 163): Western blot analysis of p38-ϒ. Lane A – human recombinant p38-α (260-360a.a., N-terminal GST tag; Novus Biologicals, cat. #: H00001432-Q01), lane B – human recombinant p38-β (with C-terminal DDK tag, Novus Biologicals, cat. #: NBL1-12870), lane C - human recombinant PKC-ϒ (1-368a.a., N-terminal GST tag, Novus Biologicals, cat. #: H00006300-P01), lane D – human recombinant p38-δ (1-366a.a., N-terminal GST tag, Novus Biologicals, cat. #: H00005603-P01). 500ng of recombinant protein was loaded in each well. Rabbit IgG R13-L Aff - Purified Hu, Ms, Rt E, ICC/IF, IP, WB
50 µl / €340.00
  DB Biotech, spol. s r.o.

Sample sizes available

MAPK12 antibody

MAPK12 Rabbit IgG Q11-T FITC Hu F
100 Tests / €560.00
  DB Biotech, spol. s r.o.

Sample sizes available

MAPK12 antibody

Formalin-fixed and paraffin-embedded human cancer tissue (hepatocarcinoma) reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P
0.4 ml / €370.00
  Acris Antibodies GmbH

MAPK12 (C-term) antibody

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with the P38 gamma antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms P, WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

MAPK12 antibody

MAPK12 Mouse Serum Hu E
50 µl / €290.00
  Abnova Taiwan Corp.

MAPK12 antibody

Western Blot analysis of MAPK12 expression in transfected 293T cell line (H00006300-T01) by MAPK12 MaxPab polyclonal antibody.

Lane 1: MAPK12 transfected lysate(40.37 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

MAPK12 antibody

Immunoprecipitation of MAPK12 transfected lysate using anti-MAPK12 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with MAPK12 MaxPab mouse polyclonal antibody (B01) (H00006300-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

MAPK12 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between MAPK12 and DLG1. HeLa cells were stained with anti-MAPK12 rabbit purified polyclonal 1:1200 and anti-DLG1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

MAPK12 antibody

Immunofluorescence of monoclonal antibody to MAPK12 on HeLa cell . [antibody concentration 10 ug/ml] Mouse IgG1 4F4-3D2 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MAPK12 antibody

MAPK12 monoclonal antibody (M02), clone 1A4 Western Blot analysis of MAPK12 expression in PC-12 ( Cat # L012V1 ). Mouse IgG1 1A4 Purified Hu, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MAPK12 antibody

Immunoperoxidase of monoclonal antibody to MAPK12 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml] Mouse IgG1 1G4 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MAPK12 antibody

Immunofluorescence of monoclonal antibody to MAPK12 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG1 2C1 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MAPK12 antibody

MAPK12 Mouse IgG1 2C1 Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

MAPK12 antibody

Immunofluorescence of monoclonal antibody to MAPK12 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG1 1B3 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MAPK12 antibody

Immunofluorescence of monoclonal antibody to MAPK12 on HeLa cell. [antibody concentration 25 ug/ml] Mouse IgG1 1G3 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MAPK12 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with MAPK12 polyclonal antibody ( Cat # PAB2786 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu, Ms E, P
0.4 ml / €390.00
  Abnova Taiwan Corp.

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