MAPK3 / ERK1 antibody

Principal name

MAPK3 / ERK1 antibody

Alternative names for MAPK3 / ERK1 antibody

MAP kinase 3, MAPK 3, ERK-1, ERT2, p44-MAPK, p44-ERK1, PRKM3, Mitogen-activated protein kinase 3, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1

SwissProt ID

P21708 (Rat), P27361 (Human), Q63844 (Mouse)

Gene ID

5595 (MAPK3), 50689 (Mapk3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Bov (Bovine), Chk (Chicken), Dros (Drosophila), Sh (Sheep), Xen (Xenopus), Rb (Rabbit), Ze (Zebrafish), Por (Porcine), African clawed frog, Mky (Monkey), Bt (Bat), Eq (Equine), Hst (Hamster), Prim (Primate), Bear, Op (Opossum)

Available hosts

Rabbit, Mouse, Goat, Sheep

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F), Dot blot (Dot), ELISA (detection) (E(detection)), ChIP assay (ChIP)

Background of MAPK3 / ERK1 antibody

The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase (1). They are both located in the cytosol and mitochondria (2).
While the role of cytosol ERK1/2 is well studied and involved in multiple cellular functions (2), the role of mitochondrial ERK1/2 remains poorly understood. Both ERK 1 and 2 are activated by MEK1 or MEK2, by dual phosphorylation of a threonine and tyrosine residue in the activation loop (TEY motif) (1, 3). Either phosphorylation alone can induce an electrophoretic mobility shift, but both are required for activation of the kinase. This dual phosphorylation is efficiently detected by phosphorylation state-specific antibody directed to the pTEpY motif. Once activated, MAP kinases phosphorylate a broad spectrum of substrates, including cytoskeletal proteins, translation regulators, transcription factors, and the Rsk family of protein kinases (4). ERK1/2 activation is generally thought to confer a survival advantage to cells (5); however there is increasing evidence that suggests that the activation of
ERK1/2 also contributes to cell death under certain conditions (5). ERK1/2 also is activated in neuronal and renal epithelial cells upon exposure to oxidative stress and toxicants or deprivation of growth factors, and inhibition of the ERK pathway blocks apoptosis (5).

General readings

Cancer Res. 2007 Dec 1;67(23):11447-54.
Suppression of proximal T cell receptor signaling and lytic function in CD8+ tumor-infiltrating T cells.
Monu N, Frey AB.
Department of Cell Biology, New York University Cancer Institute, New York University School of Medicine, New York, New York 10016, USA.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ERK1/2 antibody

Immunohistochemical analysis of ERK1/2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

ERK1/2 antibody

Immunohistochemical analysis of ERK1/2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

ERK1/2 antibody

Immunohistochemical analysis of ERK1/2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

ERK1/2 antibody

Immunofluorescent analysis of ERK1/2 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Chk, Hu, Rt, Ze ICC/IF, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

ERK1/2 antibody

Immunofluorescent analysis of ERK1/2 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Chk, Hu, Rt, Ze ICC/IF, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

ERK1/2 antibody

Immunofluorescent analysis of ERK1/2 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Chk, Hu, Rt, Ze ICC/IF, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

ERK1/2 antibody

Immunohistochemical analysis of ERK1/2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt, Ze IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

ERK1/2 antibody

Immunohistochemical analysis of ERK1/2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt, Ze IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

ERK1/2 antibody

Immunohistochemical analysis of ERK1/2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt, Ze IP, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

ERK1/2 antibody

Western blot analysis of ERK1/2 expression in mouse brain (A); rat brain (B) whole cell lysates. Mouse Aff - Purified Hu, Ms, Rt P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

ERK1/2 antibody

Western blot analysis of ERK1/2 expression in mouse brain (A); rat brain (B) whole cell lysates. Mouse Aff - Purified Hu, Ms, Rt P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

ERK1/2 antibody

Western blot analysis of ERK1/2 expression in mouse brain (A); rat brain (B) whole cell lysates. Mouse Aff - Purified Hu, Ms, Rt P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

ERK1 / ERK2 antibody

Western blot analysis of ERK1/2 in a human cell line mix, using a 1:1000 dilution of the antibody Rabbit IgG Serum Bov, Chk, Dros, Hu, Ms, Rt, Sh, Xen C, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ERK1 / ERK2 pThr202 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase antibody (pThr202) Cat.-No AP02498PU (left) or the same antibody preincubated with Blocking peptide (right). Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

ERK1 / ERK2 pThr202 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase antibody (pThr202) Cat.-No AP02498PU (left) or the same antibody preincubated with Blocking peptide (right). Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
50 µl / €240.00
  Acris Antibodies GmbH

MAPK3 / ERK1 (Internal Region) antibody

Anti-MAPK3 / ERK1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit IgG Aff - Purified Bear, Bov, Bt, Can, Elephant, Eq, Hst, Hu, Mky, Ms, Op, Platypus, Por, Prim, Rb, Rt P
50 µg / €379.00
  OriGene Technologies, Inc.

MAPK3 / ERK1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MAPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505700) Mouse IgG1 OTI4D7 Purified Can, Hu, Mky ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

MAPK3 / ERK1 antibody

Immunohistochemical analysis of paraffin-embedded breast cancer tissues using MAPK3 mouse mAb with DAB staining. Mouse IgG1 1E5 Ascites Hu, Mky, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

MAPK3 / ERK1 antibody

Human Vessels: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 3C9 Purified Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

MAPK3 / ERK1 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between MAPK3 and PTPN11. Mahlavu cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-PTPN11 mouse purified polyclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

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