MAPK3 / ERK1 antibody

Principal name

MAPK3 / ERK1 antibody

Alternative names for MAPK3 / ERK1 antibody

MAP kinase 3, MAPK 3, ERK-1, ERT2, p44-MAPK, p44-ERK1, PRKM3, Mitogen-activated protein kinase 3, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1

SwissProt ID

P21708 (Rat), P27361 (Human), Q63844 (Mouse)

Gene ID

5595 (MAPK3), 50689 (Mapk3), 26417

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Xen (Xenopus), Bov (Bovine), Rb (Rabbit), Chk (Chicken), Ze (Zebrafish), Por (Porcine), African clawed frog, Mky (Monkey), Dros (Drosophila), Sh (Sheep), Bt (Bat), Eq (Equine), Hst (Hamster), Prim (Primate), Bear, Op (Opossum)

Available hosts

Mouse, Rabbit, Goat

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Dot blot (Dot), ELISA (detection) (E(detection))

Background of MAPK3 / ERK1 antibody

The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase (1). They are both located in the cytosol and mitochondria (2).
While the role of cytosol ERK1/2 is well studied and involved in multiple cellular functions (2), the role of mitochondrial ERK1/2 remains poorly understood. Both ERK 1 and 2 are activated by MEK1 or MEK2, by dual phosphorylation of a threonine and tyrosine residue in the activation loop (TEY motif) (1, 3). Either phosphorylation alone can induce an electrophoretic mobility shift, but both are required for activation of the kinase. This dual phosphorylation is efficiently detected by phosphorylation state-specific antibody directed to the pTEpY motif. Once activated, MAP kinases phosphorylate a broad spectrum of substrates, including cytoskeletal proteins, translation regulators, transcription factors, and the Rsk family of protein kinases (4). ERK1/2 activation is generally thought to confer a survival advantage to cells (5); however there is increasing evidence that suggests that the activation of
ERK1/2 also contributes to cell death under certain conditions (5). ERK1/2 also is activated in neuronal and renal epithelial cells upon exposure to oxidative stress and toxicants or deprivation of growth factors, and inhibition of the ERK pathway blocks apoptosis (5).

General readings

Meloche S, et al,. Oncogene (2007) 26:3227-39.
Raman M, et al,. Oncogene (2007) 26:3100-12.
Roberts PJ, et al,. Oncogene (2007) 26:3291-310.
Shaul YD, et al,. Seger R. Acta (2007) 1773:1213-26.

  1. Quick-Filters:
  2. Highly Validated (3)

Items 1 to 20 of 69 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ERK1 / ERK2 antibody

  Rabbit Polyclonal Anti-Erk1/2 Antibody  
Western blot analysis of ERK1/2 in a human cell line mix, using a 1:1000 dilution of the antibody Rabbit IgG Serum Bov, Chk, Dros, Hu, Ms, Rt, Sh, Xen C, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ERK1 / ERK2 pThr202 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase antibody (pThr202) Cat.-No AP02498PU (left) or the same antibody preincubated with Blocking peptide (right). Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

ERK1 / ERK2 pThr202 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase antibody (pThr202) Cat.-No AP02498PU (left) or the same antibody preincubated with Blocking peptide (right). Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
50 µl / €240.00
  Acris Antibodies GmbH

MAPK3 / ERK1 antibody

  MAPK3 (ERK1) mouse monoclonal antibody, clone OTI4D7 (formerly 4D7)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MAPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505700) Mouse IgG1 OTI4D7 Purified Can, Hu, Mky ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

MAPK3 / ERK1 antibody

Immunohistochemical analysis of paraffin-embedded breast cancer tissues using MAPK3 mouse mAb with DAB staining. Mouse IgG1 1E5 Ascites Hu, Mky, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

MAPK3 / ERK1 antibody

Human Vessels: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 3C9 Purified Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

MAPK3 / ERK1 (N-term) antibody

  Goat Polyclonal Antibody against ERK1 / MAPK3  
TA302659 (0.3µg/ml) staining of 293 lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAPK3 / ERK1 (C-term) antibody

  Rabbit Anti-ERK/MAPK Antibody  
Western blot of rat hippocampal homogenate showing specific immunolabeling of ~42-44k ERK/MAPK protein. Rabbit IgG Aff - Purified Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAPK3 / ERK1 antibody

  Rabbit polyclonal Bi-Phospho-ERK1/2(T202/Y204) Antibody  
Western blot analysis of ERK1/2 (arrow) using rabbit polyclonal P-MAPK(T202/Y204) (Cat.#TA324783). HAOSMC cell lysates either transiently induced (Lane 2) or noninduced with the PDGF (Lane 1)(2 ug/lane). Rabbit IgG Purified Dros, Hu, Ms, Rt Dot, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

MAPK3 / ERK1 (N-term) antibody

  Rabbit polyclonal MAPK3 Antibody (N-term)  
Western blot analysis of ERK1 (arrow) using rabbit ERK1 (N-term) Pab  (Cat. #TA324941).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK1 gene (Lane 2) (Origene Technologies). Rabbit Ig Purified Hu, Ms, Rt P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

MAPK3 / ERK1 antibody

  Mouse monoclonal Erk1/2 Antibody  
Erk1/2 Antibody (Cat. #TA325139) western blot analysis in 293,MCF-7,Jurkat,mouse NIH/3T3,rat C6 cell line and mouse heart lysates (35ug/lane).This demonstrates the Erk1/2 antibody detected the Erk1/2 protein (arrow). Mouse IgG2a 784CT7.6.3 Purified Hu, Ms WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

MAPK3 / ERK1 antibody

  Rabbit Polyclonal ERK1/2 Antibody  
Western blot analysis of ERK1/2 expression in A431 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAPK3 / ERK1 antibody

  Rabbit Polyclonal erk1/2 Antibody  
Western blot analysis on COLO205 cell lysate using ERK1/2 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAPK3 / ERK1 antibody

  Rabbit Polyclonal ERK1/2 (Thr202/Tyr204) Antibody (Phospho-specific)  
Western blot analysis of ERK1/2 phosphorylation expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAPK3 / ERK1 antibody

  Rabbit Polyclonal ERK1/2 (Tyr204) Antibody (Phospho-specific)  
Western blot analysis of ERK1/2 phosphorylation expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAPK3 / ERK1 antibody

  Rabbit Polyclonal p44/42 MAP Kinase (Thr202) Antibody (Phospho-specific)  
Western blot analysis of p44/42 MAP Kinase phosphorylation expression in EGF treated A431 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAPK3 / ERK1 antibody

  Rabbit polyclonal p44 MAPK antibody  
Western blot analysis of extracts from Jurkat cells and HUVEC cells, using p44 MAPK antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAPK3 / ERK1 antibody

  Rabbit polyclonal p44/42 MAPK antibody  
Western blot analysis of extracts from HeLa cells, using ERK1/2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAPK3 / ERK1 (Internal Region) antibody

  Rabbit Polyclonal Anti-MAPK3 Antibody (Internal)  
MAPK3 / ERK1 Rabbit Aff - Purified Bear, Bov, Bt, Can, Elephant, Eq, Hst, Hu, Mky, Ms, Por, Prim, Rb, Rt P
50 µg / €400.00
  OriGene Technologies, Inc.

MAPK3 / ERK1 (Internal) antibody

  Rabbit Polyclonal Anti-MAPK3 Antibody (Internal)  
MAPK3 / ERK1 Rabbit Aff - Purified Bear, Bov, Bt, Can, Elephant, Eq, Hst, Hu, Mky, Ms, Por, Prim, Rb, Rt P
50 µg / €400.00
  OriGene Technologies, Inc.

Items 1 to 20 of 69 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4
  • LinkedIn