MAPK7 / ERK5 antibody

Principal name

MAPK7 / ERK5 antibody

Alternative names for MAPK7 / ERK5 antibody

Mitogen-activated protein kinase 7, Extracellular signal-regulated kinase 5, ERK4, Big MAP kinase 1, MAPK7

SwissProt ID

A5PKJ4 (Bovin), P0C865 (Rat), Q13164 (Human), Q9WVS8 (Mouse)

Gene ID

5598 (MAPK7)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine)

Available hosts

Rabbit, Mouse, Chicken

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Frozen Sections (C), Paraffin Sections (P), Immunoprecipitation (IP), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of MAPK7 / ERK5 antibody

ERK5 (Mitogen-activated protein kise 7, Big mitogen-activated protein kise 1) is a member of the MAPK superfamily implicated in the regulation numerous cellular processes including proliferation, differentiation, and survival. Like other MAPK family members, ERK5 contains a canonical activation loop TEY motif (Thr218/Tyr220) which is specifically phosphorylated by MAP2K5 (MEK5) in a growth factor-dependent, Ras-independent mechanism. For example, EGF stimulation promotes ERK5 phosphorylation which induces its traslocation to the nucleus where it phosphorylates MEF2C and other transcriptiol targets. ERK5 is also activated in response to granulocyte colony-stimulating factor (G-CSF) in hematopoetic progenitor cells where it promotes survival and proliferation. In neurol cells, ERK5 is required for NGF-induced neurite outgrowth, neurol homeostatis, and survival. ERK5 is thought to play a role in blood vessel integrity via maintainence of endothelial cell migration and barrier function. Although broadly expressed, research studies have shown that mice lacking erk5 display numerous cardiac defects, suggesting ERK5 plays a critical role in vascular development and homeostasis.

General readings

Marinissen, M.J. et al. (1999) A network of mitogen-activated protein kinases links G protein-coupled receptors to the c-jun promoter: a role for c-Jun NH2-terminal kinase, p38s, and extracellular signal-regulated kinase 5. Mol. Cell. Biol. 19(6):4289-301.
Kamakura, S. et al. (1999) Activation of the Protein Kinase ERK5/BMK1 by Receptor Tyrosine Kinases. Identification and characterization of a signaling pathway to the nucleus. J. Biol. Chem. 274(37):26563-26571.
Kato, Y. et al. (1998) Bmk1/Erk5 is required for cell proliferation induced by epidermal growth factor. Nature 395(6703):713-6.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MAPK7 / ERK5 antibody

Anti-MAPK7 mouse monoclonal antibody (TA502119) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK7(RC203506). Mouse IgG1 OTI3H2 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPK7 / ERK5 antibody

Anti-MAPK7 mouse monoclonal antibody (TA502148) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK7(RC203506). Mouse IgG1 OTI2E2 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPK7 / ERK5 antibody

Anti-MAPK7 mouse monoclonal antibody (TA502149) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK7(RC203506). Mouse IgG1 OTI4F9 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPK7 / ERK5 antibody

Anti-MAPK7 mouse monoclonal antibody (TA502150) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK7(RC203506). Mouse IgG2a OTI3D4 Purified Hu F, ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

MAPK7 / ERK5 antibody

Anti-MAPK7 mouse monoclonal antibody (TA502187) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK7(RC203506). Mouse IgG1 OTI4C1 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPK7 / ERK5 antibody

Anti-MAPK7 mouse monoclonal antibody (TA502188) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK7(RC203506). Mouse IgG1 OTI4D6 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPK7 / ERK5 antibody

Anti-MAPK7 mouse monoclonal antibody (TA502189) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK7(RC203506). Mouse IgG1 OTI5B9 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPK7 / ERK5 antibody

Western blot analysis of extracts of various cell lines, using MAPK7 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAPK7 / ERK5 antibody

Anti-MAPK7 mouse monoclonal antibody (TA502185) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK7(RC203506). Mouse IgG1 OTI1G1 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPK7 / ERK5 antibody

Anti-MAPK7 mouse monoclonal antibody (TA502186) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK7(RC203506). Mouse IgG1 OTI2F1 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPK7 / ERK5 antibody

Anti-MAPK7 mouse monoclonal antibody (TA502202) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK7(RC203506). Mouse IgG1 OTI1A4 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPK7 / ERK5 antibody

Anti-MAPK7 mouse monoclonal antibody (TA502203) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK7(RC203506). Mouse IgG1 OTI5C7 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPK7 / ERK5 (incl. pos. control) antibody

Detection of endogenous MAPK7 / erk5 Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. Immunoblots were probed with mab MAPK7-12F2 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). 
Lane 1: A431 
Lane 2: A549
Lane 3: SKOV3
Lane 4: OVCAR5
Lane 5: HaCaT
Lane 6: PC3 
Lane 7: HeLa 
Lane 8: HepG2 Mouse IgG1 12F2 Purified Can, Hu, Ms, Rt WB
0.1 mg / €430.00
  Acris Antibodies GmbH

MAPK7 / ERK5 antibody

MAPK7 / ERK5 Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €390.00
  Acris Antibodies GmbH

MAPK7 / ERK5 antibody

Western blot using ERK5 antibody (AP05269PU-N) on HEK293 cells transfected with human ERK5 protein. Incubated with 0.15-1.5 µg/ml of ERK5 antibody and visualized with goat antirabbit IgG antibody conjugated to alkaline phosphatase. Rabbit IgG Aff - Purified Hu, Ms WB
0.1 ml / €530.00
  Acris Antibodies GmbH

MAPK7 / ERK5 (C-term) antibody

(LEFT)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.  (RIGHT)Formalin-fixed and paraffin-embedded human kideny carcinoma tissue reacted with ERK5 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MAPK7 / ERK5 antibody

MAPK7 / ERK5 Mouse Serum Hu E
50 µl / €290.00
  Abnova Taiwan Corp.

MAPK7 / ERK5 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with MAPK7 polyclonal antibody ( Cat # PAB2891 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

MAPK7 / ERK5 antibody

Western blot using MAPK7 polyclonal antibody on 293 cells transfected with human MAPK7 protein. Rabbit Hu, Ms WB
0.1 ml / €660.00
  Abnova Taiwan Corp.

MAPK7 / ERK5 (Center) antibody

Human Adult Liver; Rabbit Anti-MAPK7 Antibody . Catalog Number: ARP57731_P050. Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver . Observed Staining: Cytoplasm in Kupffer cells only, strong signal, low tissue distribution. Primary Antibody Concentration: 1:100. Secondary Antibody: Donkey anti-Rabbit-Cy3. Secondary Antibody Concentration: 1:200. Magnification: 20X. Exposure Time: 0.5 – 2.0 sec. Protocol located in Reviews and Data.; MAPK7 antibody - middle region (ARP57731_P050) in Human Adult Liver cells using Immunohistochemistry Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

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