MAPK8 / JNK1 antibody

Principal name

MAPK8 / JNK1 antibody

Alternative names for MAPK8 / JNK1 antibody

Mitogen-activated protein kinase 8, c-Jun N-terminal kinase 1, JNK-46, JNK-1, PRKM8, Stress-activated protein kinase JNK1

SwissProt ID

P45983 (Human), P49185 (Rat), Q8QHK8 (Xenla), Q8WQG9 (Caeel), Q91Y86 (Mouse)

Gene ID

5599 (MAPK8), 26419 (Mapk8)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Bov (Bovine), Chk (Chicken), Por (Porcine), Ce (C. elegans), Mam (Mammalia)

Available hosts

Rabbit, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot)

Background of MAPK8 / JNK1 antibody

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptiol activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells By similarity. Phosphorylates heat shock factor protein 4 (HSF4). /Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptiol activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. JNK2 isoforms display different binding patterns: a-1 and a-2 preferentially bind to c-Jun, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 a-2, and JUND binds only weakly to it. /Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptiol activity. Required for stress-induced neurol apoptosis and the pathogenesis of glutamate excitotoxicity

General readings

Dasgupta, J., et al. J. Cell. Physiol. 225(1):52-62(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Gao, D., et al. J. Biol. Chem. 285(39):29965-29973(2010)
Han, J.S., et al. Anticancer Res. 30(9):3407-3412(2010)
Yang, X.H., et al. World J. Gastroenterol. 16(27):3385-3393(2010)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

JNK1/2 pThr183/pTyr185 antibody

Figure 1. Immunofluorescence staining of methanol-fixed HeLa cells using JNK1/JNK2 (phospho-Thr183/Tyr185) Antibody (#AP09471PU, Red). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

JNK1/2 pThr183/pTyr185 antibody

Figure 1. Immunofluorescence staining of methanol-fixed HeLa cells using JNK1/JNK2 (phospho-Thr183/Tyr185) Antibody (#AP09471PU, Red). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
50 µl / €240.00
  Acris Antibodies GmbH

JNK1/2/3 pThr183 antibody

Immunohistochemistry (IHC) analyzes of p-JNK1/2/3 antibody (Cat.-No.: AP20849PU-N) in paraffin-embedded human colon carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

MAPK8 / JNK1 antibody

  anti-JNK1 (JNK1) mouse monoclonal antibody, clone OTI2F10 (formerly 2F10)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK8 (RC218407, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK8. Mouse IgG2a OTI2F10 Purified Hu WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

MAPK8 / JNK1 antibody

  JNK1 (MAPK8) mouse monoclonal antibody, clone OTI1C2 (formerly 1C2)  
Anti-JNK1 mouse monoclonal antibody (TA500043) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY JNK1(RC218407). Mouse IgG2a OTI1C2 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPK8 / JNK1 antibody

  JNK1 (MAPK8) mouse monoclonal antibody, clone OTI2C6 (formerly 2C6)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-JNK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500075) Mouse IgG1 OTI2C6 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

MAPK8 / JNK1 antibody

  JNK1 (MAPK8) mouse monoclonal antibody, clone OTI4E3 (formerly 4E3)  
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-JNK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500081) Mouse IgG2a OTI4E3 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPK8 / JNK1 antibody

Western blot analysis using MAPK8 antibody Cat.-No AM06773PU-N against human MAPK8 (AA: 227-380) recombinant protein. Mouse IgG1 1E5 Purified Hu, Ms E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

MAPK8 / JNK1 antibody

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 1A2 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

MAPK8 / JNK1 antibody

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 4H6 Purified Hu E, IP, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

MAPK8 / JNK1 antibody

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2b 4H5 Purified Hu E, IP, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

MAPK8 / JNK1 antibody

  Rabbit Polyclonal antibody to JNK1 (mitogen-activated protein kinase 8)  
Sample (30 ug of whole cell lysate). A: Raji. 10% SDS PAGE. TA308811 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAPK8 / JNK1 antibody

  Rabbit polyclonal JNK1/2/3 (Thr183+Tyr185) antibody(Phospho-specific)  
Western blot analysis of extracts from 293 cells, treated with UV (5mins), using JNK1/2/3 (Phospho-Thr183+Tyr185) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAPK8 / JNK1 antibody

  Anti-MAPK8 Rabbit Polyclonal Antibody  
Predicted band size: 48 kDa . Positive control: Human lymphoma and liver cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: Human lymphoma tissue lysate Lane2: Human liver cancer tissue lysate Lysates: 40 ug per lane Primary antibody: 1/1000 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAPK8 / JNK1 antibody

  Anti-MAPK8 Rabbit Polyclonal Antibody  
Western blot analysis of Human lymphoma tissue using TA324295(MAPK8 Antibody) at dilution 1/400 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAPK8 / JNK1 antibody

  Anti-MAPK8 Rabbit Polyclonal Antibody  
Western blot analysis of A172 cell using TA324296(MAPK8 Antibody) at dilution 1/200 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAPK8 / JNK1 antibody

  Anti-MAPK8/MAPK9/MAPK10 (phospho-Thr183/Tyr185) Rabbit Polyclonal Antibody  
Predicted band size: 46; 54 kDa . Positive control: C6 cells untreated or treated with anisomycin lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1: C6 cells treated with anisomycin lysate Lane2: C6 cells untreated with anisomycin lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAPK8 / JNK1 antibody

  Rabbit Polyclonal JNK1/2/3 Antibody  
Western blot analysis of JNK1/2/3 expression in UV treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAPK8 / JNK1 antibody

  Rabbit Polyclonal SAPK/JNK Antibody  
Western blot analysis of SAPK/JNK expression in LOVO whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAPK8 / JNK1 antibody

  Rabbit Polyclonal JNK1/2/3 (Thr183+Tyr185) Antibody (Phospho-specific)  
Western blot analysis of JNK1/2/3 phosphorylation expression in UV treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

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