MAPK9 / JNK2 antibody

Principal name

MAPK9 / JNK2 antibody

Alternative names for MAPK9 / JNK2 antibody

JNK-55, PRKM9, MAP kinase 9, Mitogen-activated protein kinase 9, Stress-activated protein kinase JNK2, c-Jun N-terminal kinase 2

SwissProt ID

P45984 (Human), P49186 (Rat), P79996 (Chick), Q9WTU6 (Mouse), Q9WTU6-2

Gene ID

5601 (MAPK9), 50658 (Mapk9), 26420

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Bt (Bat), Bov (Bovine), Eq (Equine), Hst (Hamster), Mky (Monkey), Por (Porcine), Prim (Primate), Rb (Rabbit), Bear, GP (Guinea Pig), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Goat

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of MAPK9 / JNK2 antibody

The stress-activated protein kise/Jun-amino-termil kise SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, growth factors and GPCR agonists. As with the other MAPKs, the core sigling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kises (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kise. Stress sigls are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs. Altertively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germil center kise (GCK) family member. There are three SAPK/JNK genes each of which undergoes altertive splicing, resulting in numerous isoforms. SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors.

General readings

Kyriakis J M., et al. (1999) J Biol Chem. 274: 5259-62Whitmarsh., et al. (1998) Trends Biochem Sci. 23: 481-5.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MAPK9 / JNK2 antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MAPK9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505869) Mouse IgG1 OTI2H3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPK9 / JNK2 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MAPK9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505877) Mouse IgG1 OTI2D11 Purified Can, Hu, Mky, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPK9 / JNK2 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MAPK9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505909) Mouse IgG1 OTI2G3 Purified Can, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPK9 / JNK2 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MAPK9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505910) Mouse IgG1 OTI4D1 Purified Can, Hu, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPK9 / JNK2 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAPK9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505911) Mouse IgG1 OTI1A1 Purified Can, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

MAPK9 / JNK2 (1-425) antibody

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 1C1-3A8 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

MAPK9 / JNK2 antibody

Immunoperoxidase of monoclonal antibody to MAPK9 on formalin-fixed paraffin-embedded human spleen tissue. [antibody concentration 3 ug/ml] Mouse IgG1 1C1-3A8 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MAPK9 / JNK2 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between TRAF2 and MAPK9 HeLa cells were stained with anti-TRAF2 rabbit purified polyclonal 1:1200 and anti-MAPK9 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG1 2F8 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MAPK9 / JNK2 antibody

MAPK9 monoclonal antibody (M05), clone 1D6. Western Blot analysis of MAPK9 expression in human liver. Mouse IgG2a 1D6 Purified Hu, Ms, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MAPK9 / JNK2 (217-230) antibody

AP31925PU-N JNK2 antibody staining of HeLa lysate at 0.03 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

MAPK9 / JNK2 (217-230) antibody

AP31957PU-N MAPK9 / JNK2 antibody staining of Human Cerebellum lysate at 0.5 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

MAPK9 / JNK2 antibody

Predicted cell location: Cytoplasm. Positive control: Human cervical cancer tissue. Recommended dilution: 1/10-50  The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using MAPK9 antibody at dilution 1/10, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAPK9 / JNK2 antibody

Western blot analysis of Raw264.7 cell, mouse brain and heart tissue using TA322064 (MAPK9 Antibody) at dilution 1/200 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAPK9 / JNK2 antibody

Western blot analysis of Hela cell using JNK2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAPK9 / JNK2 antibody

Western blot analysis of extracts from 293 cells using phospho-MAPK9/MAPK10-T183 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAPK9 / JNK2 antibody

WB Suggested Anti-Mapk9 Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Heart Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

MAPK9 / JNK2 (incl. pos. control) antibody

MAPK9 / JNK2 Mouse IgG1 12C5 Biotin Can, Hu, Ms, Rt C, E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

MAPK9 / JNK2 (incl. pos. control) antibody

Figure 1. Detection of endogenous SAPK1alpha Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab SAPK1a/jnk2-12C5 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).  
lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2 Mouse IgG1 12C5 Purified Can, Hu, Ms, Rt C, E, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

MAPK9 / JNK2 (C-term) antibody

Brain, cortex: Formalin-Fixed Paraffin Embedded (FFPE) Rabbit IgG Aff - Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

MAPK9 / JNK2 (N-term) antibody

Brain, cortex: Formalin-Fixed Paraffin Embedded (FFPE) Rabbit IgG Aff - Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Items 1 to 20 of 57 total

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