MAPK9 / JNK2 antibody

Principal name

MAPK9 / JNK2 antibody

Alternative names for MAPK9 / JNK2 antibody

JNK-55, PRKM9, MAP kinase 9, Mitogen-activated protein kinase 9, Stress-activated protein kinase JNK2, c-Jun N-terminal kinase 2

SwissProt ID

P45984 (Human), P49186 (Rat), P79996 (Chick), Q9WTU6 (Mouse), Q9WTU6-2

Gene ID

5601 (MAPK9), 50658 (Mapk9), 26420

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Por (Porcine), Mky (Monkey), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Goat

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Dot blot (Dot), Immunoprecipitation (IP)

Background of MAPK9 / JNK2 antibody

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]. Transcript Variant: This variant (JNK2-a1) uses a different acceptor splice site in the last coding exon compared to transcript variant JNK2-a2, resulting in a frameshift and a shorter isoform (JNK2 alpha1) with a different C-terminus, compared to isoform JNK2 alpha2. The JNK2-a1 variant differs from the JNK2-b1 variant in the use of an alternate internal coding exon of the same length. Thus, JNK2 alpha1 isoform is the same length as JNK2 beta1 isoform, with a few aa differences in an internal protein segment. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Kyriakis J M., et al. (1999) J Biol Chem. 274: 5259-62Whitmarsh., et al. (1998) Trends Biochem Sci. 23: 481-5.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MAPK9 / JNK2 antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MAPK9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505869) Mouse IgG1 OTI2H3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPK9 / JNK2 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MAPK9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505877) Mouse IgG1 OTI2D11 Purified Can, Hu, Mky, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPK9 / JNK2 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MAPK9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505909) Mouse IgG1 OTI2G3 Purified Can, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPK9 / JNK2 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MAPK9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505910) Mouse IgG1 OTI4D1 Purified Can, Hu, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPK9 / JNK2 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAPK9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505911) Mouse IgG1 OTI1A1 Purified Can, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

MAPK9 / JNK2 (1-425) antibody

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 1C1-3A8 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

MAPK9 / JNK2 antibody

Immunoperoxidase of monoclonal antibody to MAPK9 on formalin-fixed paraffin-embedded human spleen tissue. [antibody concentration 3 ug/ml] Mouse IgG1 1C1-3A8 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MAPK9 / JNK2 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between TRAF2 and MAPK9 HeLa cells were stained with anti-TRAF2 rabbit purified polyclonal 1:1200 and anti-MAPK9 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG1 2F8 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MAPK9 / JNK2 antibody

MAPK9 monoclonal antibody (M05), clone 1D6. Western Blot analysis of MAPK9 expression in human liver. Mouse IgG2a 1D6 Purified Hu, Ms, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MAPK9 / JNK2 (217-230) antibody

AP31925PU-N JNK2 antibody staining of HeLa lysate at 0.03 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

MAPK9 / JNK2 (217-230) antibody

AP31957PU-N MAPK9 / JNK2 antibody staining of Human Cerebellum lysate at 0.5 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

MAPK9 / JNK2 antibody

Predicted cell location: Cytoplasm. Positive control: Human cervical cancer tissue. Recommended dilution: 1/10-50  The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using MAPK9 antibody at dilution 1/10, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAPK9 / JNK2 antibody

Western blot analysis of Raw264.7 cell, mouse brain and heart tissue using TA322064 (MAPK9 Antibody) at dilution 1/200 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAPK9 / JNK2 antibody

Western blot analysis of Hela cell using JNK2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAPK9 / JNK2 antibody

Western blot analysis of extracts from 293 cells using phospho-MAPK9/MAPK10-T183 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAPK9 / JNK2 antibody

WB Suggested Anti-Mapk9 Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Heart Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

MAPK9 / JNK2 (incl. pos. control) antibody

MAPK9 / JNK2 Mouse IgG1 12C5 Biotin Can, Hu, Ms, Rt C, E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

MAPK9 / JNK2 (incl. pos. control) antibody

Figure 1. Detection of endogenous SAPK1alpha Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab SAPK1a/jnk2-12C5 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).  
lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2 Mouse IgG1 12C5 Purified Can, Hu, Ms, Rt C, E, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

MAPK9 / JNK2 (C-term) antibody

Brain, cortex: Formalin-Fixed Paraffin Embedded (FFPE) Rabbit IgG Aff - Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

MAPK9 / JNK2 (N-term) antibody

Brain, cortex: Formalin-Fixed Paraffin Embedded (FFPE) Rabbit IgG Aff - Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

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