MAPK9 / JNK2 antibody
Principal name
MAPK9 / JNK2 antibody
Alternative names for MAPK9 / JNK2 antibody
JNK-55, PRKM9, MAP kinase 9, Mitogen-activated protein kinase 9, Stress-activated protein kinase JNK2, c-Jun N-terminal kinase 2
Ncbi ID
NP_001128516.1, 9606, 10116, NP_002743, NM_016961, NP_001128516, NP_620707.1, NM_002752, NP_002743.3, NM_139068, NM_139070, NP_620707, NP_620709, NP_001157143.1, NM_207692, NM_001163671
Available reactivities
Available hosts
Available applications
Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))
Background of MAPK9 / JNK2 antibody
The stress-activated protein kise/Jun-amino-termil kise SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, growth factors and GPCR agonists. As with the other MAPKs, the core sigling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kises (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kise. Stress sigls are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs. Altertively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germil center kise (GCK) family member. There are three SAPK/JNK genes each of which undergoes altertive splicing, resulting in numerous isoforms. SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors.
General readings
Kyriakis J M., et al. (1999) J Biol Chem. 274: 5259-62Whitmarsh., et al. (1998) Trends Biochem Sci. 23: 481-5.
Figure 1. Detection of endogenous SAPK1alpha Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab SAPK1a/jnk2-12C5 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2
Immunoperoxidase of monoclonal antibody to MAPK9 on formalin-fixed paraffin-embedded human spleen tissue. [antibody concentration 3 ug/ml]
Western Blot analysis of MAPK9 expression in transfected 293T cell line by MAPK9 monoclonal antibody (M01), clone 1C1-3A8.Lane 1: MAPK9 transfected lysate(48.1 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of MAPK9 over-expressed 293 cell line, cotransfected with MAPK9 Validated Chimera RNAi ( Cat # H00005601-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MAPK9 monoclonal antibody (M01) clone 1C1-3A8 (Cat # H00005601-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Detection limit for recombinant GST tagged MAPK9 is approximately 3ng/ml as a capture antibody.
Proximity Ligation Analysis (PLA) of protein-protein interactions between TRAF2 and MAPK9 HeLa cells were stained with anti-TRAF2 rabbit purified polyclonal 1:1200 and anti-MAPK9 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Immunofluorescence of monoclonal antibody to MAPK9 on HeLa cell. [antibody concentration 10 ug/ml]
MAPK9 monoclonal antibody (M02), clone 2F8 Western Blot analysis of MAPK9 expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged MAPK9 is approximately 0.03ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to MAPK9 on HeLa cell. [antibody concentration 10 ug/ml]
MAPK9 monoclonal antibody (M03), clone 3C12 Western Blot analysis of MAPK9 expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged MAPK9 is approximately 0.3ng/ml as a capture antibody.
MAPK9 monoclonal antibody (M05), clone 1D6. Western Blot analysis of MAPK9 expression in human liver.
MAPK9 monoclonal antibody (M05), clone 1D6. Western Blot analysis of MAPK9 expression in Raw 264.7(Cat # L024V1 ).
MAPK9 monoclonal antibody (M05), clone 1D6. Western Blot analysis of MAPK9 expression in HepG2 ( Cat # L019V1 ).
MAPK9 monoclonal antibody (M05), clone 1D6. Western Blot analysis of MAPK9 expression in PC-12(Cat # L012V1 ).
Brain, cortex: Formalin-Fixed Paraffin Embedded (FFPE)
Brain, cortex: Formalin-Fixed Paraffin Embedded (FFPE)
Brain, cortex, capillary: Formalin-Fixed Paraffin-Embedded (FFPE)
Western blot analysis using MAPK9 monoclonal antibody, clone 341P16 ( Cat # MAB1909 ) on Jurkat lysate ( 1 ) , mouse 3T3-L1 lysate ( 2 ) and rat PC-12 lysate ( 3 ).
Western Blot analysis of MAPK9 expression in transfected 293T cell line (H00005601-T01) by MAPK9 MaxPab polyclonal antibody.Lane 1: MAPK9 transfected lysate(48.10 KDa).Lane 2: Non-transfected lysate.
Western blot (WB) analysis of JNK2 antibody (Cat.-No.: AP20523PU-N) in extracts from A549 cells.
Mouse Heart; WB Suggested Anti-Mapk9 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Heart; Mapk9 antibody - N-terminal region (ARP57732_P050) in Mouse Heart cells using Western Blot
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of MAPK9 over-expressed 293 cell line, cotransfected with MAPK9 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MAPK9 monoclonal antibody AM31822PU-N.
Western Blot analysis of MAPK9 expression in transfected 293T cell line by MAPK9 monoclonal antibody AM31822PU-N
Detection limit for recombinant GST tagged MAPK9 is approximately 3ng/ml as a capture antibody.
AP31925PU-N JNK2 antibody staining of HeLa lysate at 0.03 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
AP31957PU-N MAPK9 / JNK2 antibody staining of Human Cerebellum lysate at 0.5 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MAPK9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505869)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAPK9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505869)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MAPK9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505869)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MAPK9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505869)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MAPK9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505869)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK9 (RC207608, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK9.
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MAPK9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505877)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAPK9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505877)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK9 (RC207608, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK9.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-MAPK9 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MAPK9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505909)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAPK9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505909)
Anti-MAPK9 mouse monoclonal antibody (TA505909) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK9(RC207608).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK9 (RC207608, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK9.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-MAPK9 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MAPK9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505910)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAPK9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505910)
Anti-MAPK9 mouse monoclonal antibody (TA505910) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK9(RC207608).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK9 (RC207608, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK9.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-MAPK9 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAPK9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505911)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MAPK9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505911)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MAPK9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505911)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-MAPK9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505911)
Anti-MAPK9 mouse monoclonal antibody (TA505911) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK9(RC207608).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK9 (RC207608, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK9.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-MAPK9 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Predicted cell location: Cytoplasm. Positive control: Human cervical cancer tissue. Recommended dilution: 1/10-50 The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using MAPK9 antibody at dilution 1/10, on the right is treated with the synthetic peptide. (Original magnification:x200)
Western blot analysis of Raw264.7 cell, mouse brain and heart tissue using TA322064 (MAPK9 Antibody) at dilution 1/200
Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using TA322064 (MAPK9 Antibody)(left image) at dilution 1/30, the image on the right is treated with the synthetic peptide.(Original magnification: x200)
Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TA322064 (MAPK9 Antibody)(left image) at dilution 1/30, the image on the right is treated with the synthetic peptide.(Original magnification: x200)
Western blot analysis of Hela cell using JNK2 antibody.
Immunofluorescence analysis of U2OS cell using MAPK9 antibody. Blue: DAPI for nuclear staining.
Western blot analysis of extracts from 293 cells using phospho-MAPK9/MAPK10-T183 antibody.
WB Suggested Anti-Mapk9 Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Heart
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA344631 | MAPK9 / JNK2 antibody |
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| Rabbit Polyclonal Anti-MAPK9 Antibody - N-terminal region | |||||||
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Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze | WB |
50 µg /
€360.00
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| OriGene Technologies, Inc. | |||||||
