MAPKAP Kinase-5 antibody

Principal name

MAPKAP Kinase-5 antibody

Alternative names for MAPKAP Kinase-5 antibody

MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAPK5, PRAK, MAP kinase-activated protein kinase 5

SwissProt ID

O54992 (Mouse), Q8IW41 (Human)

Gene ID

8550 (MAPKAPK5), 17165 (Mapkapk5)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Por (Porcine), Rt (Rat)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of MAPKAP Kinase-5 antibody

PRAK (p38-regulated /activated kinase), also referred to as mitogen-activated protein kinase (MAPK)-activated protein kinase (MAPKAPK)-5, is an ubiquitously expressed serine/threonine kinase regulated by p38α and p38β MAP kinases. Activated JNK, p38γ or p38δ are unable to induce phosphorylation of PRAK in vitro. Phosphorylation of PRAK occurs in vivo in response to p38 activation by stress-related extracellular stimuli including UV light, oxidation and proinflammatory cytokines. Two other substrates for p38, MAPKAPK-2 and MAPKAPK-3/3pK, share approximately 45% sequence homology with PRAK including the phosphorylation motif recognized by p38, Lys-X-Thr-Pro. Activated PRAK has been shown to specifically phosphorylate HSP 27 in vitro, suggesting that the protein may play a role in stress-induced small heat shock protein phosphorylation in vivo.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MAPKAP Kinase-5 antibody

AP32808PU-N (5 µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, IP, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

MAPKAP Kinase-5 (192-206) antibody

AP32809PU-N (5 µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, IP, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

MAPKAP Kinase-5 antibody

Sample(30 ug of whole cell lysate). A:293T. B:H1299. 7.5% SDS PAGE. TA308640 diluted at 1:500 Rabbit IgG Aff - Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAPKAP Kinase-5 antibody

Western blot analysis of extracts from MCF-7 cells, using MAPKAPK5 (Ab-182) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAPKAP Kinase-5 antibody

Western blot analysis on K562 cell lysate using MAPKAPK5 Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAPKAP Kinase-5 antibody

Western blot analysis on K562 cell lysate using Phospho-MAPKAPK5(Thr182) Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAPKAP Kinase-5 antibody

Western blot analysis on K562 cell lysate using Phospho-MAPKAPK5(Thr182) Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAPKAP Kinase-5 antibody

MAPKAP Kinase-5 Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAPKAP Kinase-5 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPKAPK5 (RC216715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPKAPK5. Mouse IgG2a OTI5E1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPKAP Kinase-5 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPKAPK5 (RC216715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPKAPK5. Mouse IgG2b OTI1B4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPKAP Kinase-5 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPKAPK5 (RC216715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPKAPK5. Mouse IgG2b OTI1A12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPKAP Kinase-5 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPKAPK5 (RC216715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPKAPK5. Mouse IgG1 OTI1H3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPKAP Kinase-5 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPKAPK5 (RC216715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPKAPK5. Mouse IgG2a OTI1D11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPKAP Kinase-5 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPKAPK5 (RC216715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPKAPK5. Mouse IgG2a OTI4G4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPKAP Kinase-5 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPKAPK5 (RC216715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPKAPK5. Mouse IgG2a OTI1B3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPKAP Kinase-5 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPKAPK5 (RC216715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPKAPK5. Mouse IgG2a OTI1A7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPKAP Kinase-5 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPKAPK5 (RC216715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPKAPK5. Mouse IgG1 OTI1F5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPKAP Kinase-5 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPKAPK5 (RC216715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPKAPK5. Mouse IgG2b OTI4H4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPKAP Kinase-5 antibody

Immunohistochemical analysis of paraffin-embedded human liver carcinoma (A), esophagus carcinoma (B), normal spleen tissue(C), breast carcinoma (D), showing nuclear and cytoplasmic localization using PRAK antibody Cat.-No AM06183SU-N with DAB staining. Mouse IgG1 7H10B4 Ascites Hu P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

MAPKAP Kinase-5 pSer93 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

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