MAPKK 2 antibody

Principal name

MAPKK 2 antibody

Alternative names for MAPKK 2 antibody

MAP kinase kinase 2, ERK activator kinase 2, MAPK/ERK kinase 2, MEK2, MKK2, MAP kinase kinase 2, Dual specificity mitogen-activated protein kinase kinase 2

SwissProt ID

P36506 (Rat), P36507 (Human), Q63932 (Mouse), Q90891 (Chick)

Gene ID

5605 (MAP2K2)

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Mky (Monkey)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), EPair for Elisa (EPair), Flow Cytometry (F), Frozen Sections (C)

Background of MAPKK 2 antibody

MAP2K2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in MAP2K2 gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene.The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Crews C M, et al. (1992) Science. 258:478-480.
Alessi D R, et al. (1994) EMBO J. 13:1610-1619.
Rosen L B, et al. (1994) Neuron. 12:1207-1221.
Cowley S, et al. (1994) Cell. 77:841-852.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MAPKK 2 antibody

The anti-MAP2K2 Pab (Cat. #TA325009) is used in Western blot to detect MAP2K2 in mouse liver tissue lysate. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

MAPKK 2 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAP2K2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500466) Mouse IgG1 OTI5F6 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPKK 2 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MAP2K2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500471) Mouse IgG1 OTI8G6 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPKK 2 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MAP2K2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505680) Mouse IgG2a OTI1A2 Purified Can, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

MAPKK 2 antibody

Immunohistochemistry-Paraffin: MEK2 Antibody (8G6) [NBP1-47836] - Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-MAP2K2mouse monoclonal antibody. (Dilution 1:50) Mouse 8G6 Aff - Purified Hu F, P, WB
0.1 ml / €530.00
  Novus Biologicals Inc.

MAPKK 2 antibody

Predicted band size: 44 kDa . Positive control: Hela, NIH/3T3 and Jurkat cell lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lane1: Hela cell lysate Lane2: NIH/3T3 cell lysate Lane3: Jurkat cell lysate Lysates: 40 ug per lane Primary antibody: 1/1900 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAPKK 2 antibody

Predicted band size: 44 kDa . Positive control: 293T and NIH/3T3 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: 293T cell lysate Lane2: NIH/3T3 cell lysate Lysates: 40 ug per lane Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAPKK 2 antibody

Western blot analysis of MEK2 expression in ovarycancer whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAPKK 2 antibody

TA326316 (0.3µg/ml) staining of Daudi (A), Jurkat (B), K562 (C) and Molt4 (D) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAPKK 2 antibody

Western blot analysis of extracts of various cell lines, using MEK2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAPKK 2 antibody

Anti-MAP2K2 mouse monoclonal antibody (TA505716) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K2(RC201827). Mouse IgG2a OTI5G8 Purified Can, Hu, Mky, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPKK 2 antibody

Anti-MAP2K2 mouse monoclonal antibody (TA505762) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K2(RC201827). Mouse IgG1 OTI5B4 Purified Can, Hu, Ms ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MAPKK 2 (incl. pos. control) antibody

Detection of endogenous MEK2 Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab MEK2-8E8(0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2 Mouse IgG1 8E8 Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

MAPKK 2 antibody

Immunofluorescence analysis of Hela cells using anti-MAP2K2 antibody Cat.-No AM06400SU-N (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye. Mouse IgG1 7F5 Ascites Hu, Ms, Rt E, ICC/IF, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

MAPKK 2 pThr394 antibody

MAPKK 2 Rabbit Aff - Purified Hu, Ms, Rt E, IP, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

MAPKK 2 pThr394 antibody

Immunohistochemical staining of paraffin-embedded human breast carcinoma tissue using MEK-2 (pThr394) antibody (left) or the same antibody preincubated with blocking peptide (right) Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

MAPKK 2 pThr394 antibody

Immunohistochemical staining of paraffin-embedded human breast carcinoma tissue using MEK-2 (pThr394) antibody (left) or the same antibody preincubated with blocking peptide (right) Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
50 µl / €240.00
  Acris Antibodies GmbH

MAPKK 2 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK2 antibody (AP02552PU-S; AP02552PU-N). Rabbit Aff - Purified Hu, Rt P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

MAPKK 2 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK2 antibody (AP02552PU-S; AP02552PU-N). Rabbit Aff - Purified Hu, Rt P, WB
50 µl / €230.00
  Acris Antibodies GmbH

MAPKK 2 antibody

Immunohistochemistry (IHC) analyzes of MEK2 antibody (Cat.-No.: AP06221PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt IP, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

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