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MARCKS antibody

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Principal name

MARCKS antibody

Alternative names for MARCKS antibody

Myristoylated alanine-rich C-kinase substrate, MACS, PRKCSL

SwissProt ID

P12624 (Bovin), P16527 (Chick), P26645 (Mouse), P29966 (Human), P30009 (Rat)

Gene ID

4082 (MARCKS), 25603 (Marcks), 17118

Ncbi ID

NP_002347.5, 9606, 5874, 10116, NP_002347, NM_002356, ACE87588.1, NP_032564.1

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Xen (Xenopus), Ze (Zebrafish)

Available hosts

Rabbit, Mouse, Rat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Background of MARCKS antibody

Actin binding protein MARCKS (myristoylated alanine-rich C kise substrate) is a major substrate of PKC, and alterations in PKC-MARCKS-actin axis are critical to several biological processes such as motility, proliferation, invasion, adhesion, cell spreading phagocytosis, secretion and membrane trafficking. Intracellular MARCKS is normally attached to cytoplasmic face of plasma membrane and when phosphorylated by activated PKC, MARCKS translocates from plasma membrane to cytoplasm. MARCKS regulates cell movement by controlling actin cytoskeleton homeostasis and filopodium/lamellipodium formation. MARCKS binds to F-actin, but not to monomeric G-actin, independently of its phosphorylation status and when unphosphorylated, it induces cell migration. MAPK8 can phosphorylate MARCKS which leads to induction of actin bundles formation/stabilization followed by reduction in actin plasticity and restricted cell movement including neurol/cancer cell migration. MARCKS has also been proposed to be involved in coupling the PKC and calmodulin sigling systems. MARCKS is implicated in the pathogenesis of various maligncies such as cancer of pituitary, thyroid, breast, colon, and small intestine as well as melanoma, glioblastoma multiforme cancer and cholangiocarcinoma.

General readings

1. Rodriguez Peña MJ  et al. MARCKS Protein Is Phosphorylated and Regulates Calcium Mobilization during Human Acrosomal Exocytosis. PLoS One 8:e64551 (2013). WB ; Human . PubMed: 23704996

H00004082-M06 - MARCKS
Immunoperoxidase of monoclonal antibody to MARCKS on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml]
H00004082-M06 - MARCKS
Immunofluorescence of monoclonal antibody to MARCKS on HeLa cell. [antibody concentration 10 ug/ml]
H00004082-M06 - MARCKS
MARCKS monoclonal antibody (M06), clone 2C2. Western Blot analysis of MARCKS expression in Raw 264.7. (The predicted M.W. of MARCKS is 29 to 39 KDa, but it seems the actual M.W. in vivo is between 68 to 90 KDa in different species. http://www.pnas.org/content/88/6/2505.full.pdf , http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=177061)
H00004082-M06 - MARCKS
Detection limit for recombinant GST tagged MARCKS is approximately 0.03ng/ml as a capture antibody.
AP02531PU-S - MARCKS
Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using MARCKS pSer158 antibody AP02531PU (Red).
AP02531PU-S - MARCKS
Figure 1. Western blot analysis of extract from starved NIH/3T3 cells, using MARCKS antibody AP02779PU (Lane 1 and 2) and MARCKS pSer158 antibody AP02531PU (Lane 3 and 4).
AP02758PU-S - MARCKS
Western blot analysis of extract from HeLa cells, using MARCKS antibody.
AP02779PU-S - MARCKS
Immunofluorescence staining of methanol-fixed HeLa cells using MARCKS antibody (Red).
AP02779PU-S - MARCKS
Western Blot analysis of extracts from 3T3 cells using MARCKS antibody and the same antibody preincubated with blocking peptide
AP07425PU-N - MARCKS
Brain, cortex: Formalin-Fixed Paraffin Embedded (FFPE)
H00004082-M04 - MARCKS
MARCKS monoclonal antibody (M04), clone 2H4. Western Blot analysis of MARCKS expression in Raw 264.7 ( Cat # L024V1 ).
H00004082-M04 - MARCKS
Detection limit for recombinant GST tagged MARCKS is approximately 0.1ng/ml as a capture antibody.
AP01344PU-N - MARCKS
Western blot (WB) analysis of MARCKS antibody (Cat.-No.: AP01344PU-N) in extracts from mouse brain cells.
AP12245PU-N - MARCKS
Western blot analysis of MARCKS Antibody (C-term) Cat.-No AP12245PU-N in Hela cell line lysates (35ug/lane). MARCKS (arrow) was detected using the purified Pab.
MAB1713 - MARCKS
Western blot analysis of extract from C6 cells, using Marcks monoclonal antibody, clone MAR 11/2 ( Cat # MAB1713 ) . Lane 1: C6 cell activated by beta-PMA 0 min, lane 2: 15 min, lane 3: 30 min, lane 4: 45 min, lane 5: 60 min and lane 6: C6 cell no treatment (DMSO).
PAB12564 - MARCKS
View of mixed neuron/glial cultures stained with our MARCKS antibody ( red ) and MARCKS polyclonal antibody ( Cat # PAB12564 ), our antibody to MAP2 made in chicken. Note that the MARCKS antibody stains vesicular structures both in the glial cells and in the dendrites of the neurons, which are strongly stained with the MAP2 antibody.
PAB12564 - MARCKS
Western blot analysis of MARCKS in whole rat cortex ( Co ), cerebellum ( Ce ), brain stem ( BS ) and spinal cord ( SC ) homogenate stained with MARCKS polyclonal antibody ( Cat # PAB12564 ) at dilution of 1 : 10,000.
PAB9642 - MARCKS
Western blot of rat brain lysate showing specific immunolabeling of the ~87k Marcks protein phosphorylated at Serr152,156 (Control, lane 1). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase, lane 2). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the Marcks Ser152,156 antibody. The immunolabeling is completely eliminated by treatment with l-Ptase.
AP08686PU-N - MARCKS
Figure 1. Western blot of rat brain lysate showing specific immunolabeling of the ~87k MARCKS protein phosphorylated at Serr152,156 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the MARCKS Ser152,156 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.
PAB14167 - MARCKS
Western blot of whole rat cortex ( Lane 1 ), cerebellum ( Lane 2 ), brain stem ( Lane 3 ) and spinal cord ( Lane 4 ) homogenate stained with MARCKS polyclonal antibody ( Cat # PAB14167 ), at dilution of 1 : 10,000.A prominent band running with an apparent SDS-PAGE molecular weight of ~ 80kDa corresponds to MARCKS.
PAB14167 - MARCKS
View of mixed neuron/glial cultures stained with MARCKS polyclonal antibody ( Cat # PAB14167 ) ( red ) and Chicken MAP2 polyclonal antibody.Note that the MARCKS antibody stains vesicular structures both in the glial cells and in the dendrites of the neurons, which are strongly stained with the MAP2 antibody.
AP09469PU-S - MARCKS
Figure 1. Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using MARCKS pSer170 antibody AP09469PU
AP09469PU-S - MARCKS
Figure 2. Western blot analysis of extracts from 293, A2780 and Huvec cells, using MARCKS pSer170 antibody AP09469PU.
AP09479PU-S - MARCKS
Figure 1. Immunofluorescence staining of methanol-fixed HeLa cells using MARCKS pSer162 Antibody (#AP09479PU, Red).
AP20755PU-N - MARCKS
Immunohistochemistry (IHC)analyzes of MARCKS antibody (Cat.-No.: AP20755PU-N) in paraffin-embedded human brain tissue.
AP20755PU-N - MARCKS
Western blot (WB) analyzes of MARCKS antibody (Cat.-No.: AP20755PU-N) in extracts from HeLa cells.
A7142-1 - MARCKS
Western blot analysis of extracts from K562 cells treated with EGF 200ng/ml 30', using MARCKS (Phospho-Ser158) Antibody. The lane on the right is treated with the synthesized peptide.
A7143-1 - MARCKS
Immunohistochemistry analysis of paraffin-embedded human brain, using MARCKS (Phospho-Ser163) Antibody. The picture on the right is treated with the synthesized peptide.
B7142-1 - MARCKS
Immunofluorescence analysis of A549 cells, using MARCKS (Ab-158) Antibody. The picture on the right is treated with the synthesized peptide.
B7142-1 - MARCKS
Western blot analysis of extracts from Jurkat cells, using MARCKS (Ab-158) Antibody. The lane on the right is treated with the synthesized peptide.
B7143-1 - MARCKS
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MARCKS (Ab-163) Antibody. The picture on the right is treated with the synthesized peptide.
B7143-1 - MARCKS
Western blot analysis of extracts from HepG2 cells, treated with LPS 100ng/ml 30', using MARCKS (Ab-163) Antibody. The lane on the right is treated with the synthesized peptide.
AM31095PU-N - MARCKS
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM31095PU-N - MARCKS
Immunofluorescence of monoclonal antibody to MARCKS on HeLa cell. [antibody concentration 10 µg/ml]
AM31095PU-N - MARCKS
MARCKS monoclonal antibody clone 2C2. Western Blot analysis of MARCKS expression in Raw 264.7. (The predicted M.W. of MARCKS is 29 to 39 KDa, but it seems the actual M.W.
AM31095PU-N - MARCKS
Detection limit for recombinant GST tagged MARCKS is approximately 0.03ng/ml as a capture antibody.
ARP60325_P050 - MARCKS
Human Fetal liver; WB Suggested Anti-MARCKS Antibody. . Titration: 1.0 ug/ml. . Positive Control: Fetal Liver; MARCKS antibody - C-terminal region (ARP60325_P050) in Human Fetal liver cells using Western Blot
ARP60326_P050 - MARCKS
Human SHSY5Y; . Sample: SH-SY5Y cells 2.0x105 cells. Primary dilution: 1:2500. Secondary Antibody: HRP-conjugated goat anti-rabbit IgG. Secondary dilution: 1:5000. Image Submitted by:Atsuhiro Tanabe. Pharma Sci Kitasato University .; MARCKS antibody - C-terminal region (ARP60326_P050) in Human SHSY5Y cells using Western Blot
ARP60326_P050 - MARCKS
Human SHSY5Y; MARCKS antibody - C-terminal region (ARP60326_P050) validated by WB using SHSY5Y cell lysate. MARCKS is strongly supported by BioGPS gene expression data to be expressed in Human SHSY5Y cells.; MARCKS antibody - C-terminal region (ARP60326_P050) in Human SHSY5Y cells using Western Blot
CPA1701-100ul - MARCKS
Western blot analysis of MARCKS (pS163) expression in MCF7 PMA-treated (A); SP2/0 PMA-treated (B); PC12 PMA-treated (C) whole cell lysates.
CPA1702-100ul - MARCKS
Western blot analysis of MARCKS expression in HeLa (A); RAW264.7 (B) whole cell lysates.
TP310277 - MARCKS
TA309163 - MARCKS
Western blot of rat cortex lysate showing specific immunolabeling of the ~80k MARCKS protein.
TA309163 - MARCKS
Mixed neuron/glial cultures stained with MARCKS antibody (red) anti-MAP2 (green)
TA336534 - MARCKS
Western Blot: MARCKS Antibody [TA336534] - Western blot of whole rat cortex (Co), cerebellum (Ce), brain stem (BS) and spinal cord (SC) homogenate stained with RPCA-MARCKS, at dilution of 1:10,000. A prominent band running with an apparent SDS-PAGE molecu
TA336534 - MARCKS
Immunocytochemistry/Immunofluorescence: MARCKS Antibody [TA336534] - View of mixed neuron/glial cultures stained with our MARCKS antibody (red) and NB300-213, our antibody to MAP2 made in chicken. Note that the MARCKS antibody stains vesicular structures
TA333250 - MARCKS
Western blot analysis of extracts from MCF cells, using phospho-MARCKS-S170 antibody.
TA333249 - MARCKS
Western blot analysis of extracts from 293 cells, using Phospho-MARCKS-S162 antibody.
TA333248 - MARCKS
Western blot analysis of extracts from 3T3 cells using Phospho-MARCKS-S158 antibody.
TA314170 - MARCKS
Western blot analysis of extracts from K562 cells, treated with EGF (200ng/ml, 30mins), using MARCKS (Phospho-Ser158) antibody.The lane on the right is treated with the synthesized peptide.
TA314169 - MARCKS
Immunohistochemical analysis of paraffin-embedded human brain tissue using MARCKS (phospho-Ser162) antibody.The picture on the right is treated with the synthesized peptide.
TA309164 - MARCKS
Western blot of rat brain lysate showing specific immunolabeling of the ~87k MARCKS protein phosphorylated at Serr152,156 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the MARCKS Ser152,156 antibody. The immunolabeling is completely eliminated by treatment with λ-Ptase.
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MARCKS antibody
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 2C2 Purified Hu, Ms E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH
+3 additional images

Highly validated antibody!

MARCKS antibody
  Rabbit Anti-MARCKS (Ser152,156) Antibody (Phospho-Specific)  
Western blot of rat brain lysate showing specific immunolabeling of the ~87k MARCKS protein phosphorylated at Serr152,156 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the MARCKS Ser152,156 antibody. The immunolabeling is completely eliminated by treatment with λ-Ptase. Rabbit IgG Purified Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MARCKS antibody
  Rabbit polyclonal MARCKS (Ser162) antibody(Phospho-specific)  
Immunohistochemical analysis of paraffin-embedded human brain tissue using MARCKS (phospho-Ser162) antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

MARCKS antibody
  Rabbit polyclonal MARCKS (Ser158) antibody(Phospho-specific)  
Western blot analysis of extracts from K562 cells, treated with EGF (200ng/ml, 30mins), using MARCKS (Phospho-Ser158) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MARCKS antibody
  Rabbit Polyclonal Phospho-MARCKS (Ser158) Antibody (Phospho-specific)  
MARCKS Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MARCKS antibody
  Rabbit Polyclonal MARCKS Antibody  
MARCKS Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MARCKS antibody
  Phospho-MARCKS-S158 Rabbit Polyclonal Antibody  
Western blot analysis of extracts from 3T3 cells using Phospho-MARCKS-S158 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

MARCKS antibody
  Phospho-MARCKS-S162 Rabbit Polyclonal Antibody  
Western blot analysis of extracts from 293 cells, using Phospho-MARCKS-S162 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

MARCKS antibody
  Phospho-MARCKS-S170 Rabbit Polyclonal Antibody  
Western blot analysis of extracts from MCF cells, using phospho-MARCKS-S170 antibody. Rabbit IgG Purified Hu
0.1 mg / €325.00
  OriGene Technologies, Inc.

MARCKS antibody
  Rabbit Polyclonal MARCKS Antibody  
Western Blot: MARCKS Antibody [TA336534] - Western blot of whole rat cortex (Co), cerebellum (Ce), brain stem (BS) and spinal cord (SC) homogenate stained with RPCA-MARCKS, at dilution of 1:10,000. A prominent band running with an apparent SDS-PAGE molecu Rabbit Purified Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.
+1 additional image

MARCKS pSer152/156 antibody
MARCKS Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €430.00
  Acris Antibodies GmbH

MARCKS antibody
Western blot (WB) analysis of MARCKS antibody (Cat.-No.: AP01344PU-N) in extracts from mouse brain cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

MARCKS pSer158 antibody
Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using MARCKS pSer158 antibody AP02531PU (Red). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / €320.00
  Acris Antibodies GmbH
+1 additional image

MARCKS pSer158 antibody
Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using MARCKS pSer158 antibody AP02531PU (Red). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
50 µl / €240.00
  Acris Antibodies GmbH
+1 additional image

MARCKS antibody
Western blot analysis of extract from HeLa cells, using MARCKS antibody. Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €320.00
  Acris Antibodies GmbH

MARCKS antibody
Western blot analysis of extract from HeLa cells, using MARCKS antibody. Rabbit Aff - Purified Hu, Ms WB
50 µl / €240.00
  Acris Antibodies GmbH

MARCKS antibody
Immunofluorescence staining of methanol-fixed HeLa cells using MARCKS antibody (Red). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / €320.00
  Acris Antibodies GmbH
+1 additional image

MARCKS antibody
Immunofluorescence staining of methanol-fixed HeLa cells using MARCKS antibody (Red). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
50 µl / €240.00
  Acris Antibodies GmbH
+1 additional image

MARCKS pSer162 antibody
Brain, cortex: Formalin-Fixed Paraffin Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

MARCKS pSer170 antibody
Figure 1. Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using MARCKS pSer170 antibody AP09469PU Rabbit Aff - Purified Hu P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH
+1 additional image

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