MARK2 antibody

Principal name

MARK2 antibody

Alternative names for MARK2 antibody

Serine/threonine-protein kinase, 2, ELKL motif kinase, EMK1, PAR1 homolog, MARK2, EMK1, MAP/microtubule affinity-regulating kinase

SwissProt ID

O08679 (Rat), Q05512 (Mouse), Q7KZI7 (Human)

Gene ID

2011 (MARK2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of MARK2 antibody

MARK2 refers to MAP/microtubule affinity-regulating kinase 2 isoform a [Homo sapiens]. EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule
stability and cancer. Several cDNA clones have been isolated that encoded two isoforms of the human ser/thr protein kinase EMK1 called MARK2. These isoforms were characterized by the presence of a 162-bp alternative exon that gave rise to two forms, one containing the exon and the other one lacking it. Both forms were found to be coexpressed in a number of selected cell lines and tissue samples. Human MARK2 was shown to be encoded by a single mRNA ubiquitously expressed. This transcription variant includes the alternative exon in the coding region and therefore codes for a longer protein. Multiple splice variants exist for this protein.
Role in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells.

General readings

Depasquale I, Thompson WD. Prognosis in human melanoma: PAR-1 expression is superior to other coagulation components and VEGF. Histopathology. 2008 Mar;52(4):500-9. PMID: 18315603

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MARK2 antibody

TA303346 (0.3µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MARK2 antibody

Western blot analysis of extracts from COS-7 cells, using MARK2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MARK2 pThr595 antibody

Western blot using Affinity Purified anti-MARK2 pT595 antibody shows detection of a band at ~82 kDa corresponding to phosphorylated MARK2 (arrowhead). HEK293 cells were transfected with pCMV-3xFlag-MARK2 plus pCMV-Flag-PKC-zeta (lane 3) or pCMV-3xFlag-MARK2 only (lane 4). Total cell lysates were run in a 4-12% Nupage SDS-gel and probed with anti-MARK2 pT595. An untransfected HEK293 cell lysate was used as a negative control (lane 2). MW markers are shown for size comparison (lane 1). PKC-zeta appears to be involved in the phosphorylation of T595 on MARK2 as increased amounts of phosphospecific staining are observed in lysates from cells transfected with both MARK2 and PKCzeta. Rabbit IgG Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

MARK2 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MARK2 antibody

Western Blot analysis of MARK2 expression in transfected 293T cell line (H00002011-T02) by MARK2 MaxPab polyclonal antibody.

Lane 1: MARK2 transfected lysate(84.30 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

MARK2 antibody

Detection limit for recombinant GST tagged MARK2 is approximately 0.03ng/ml as a capture antibody. Mouse IgG2a 3B12 Purified Hu, Ms, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MARK2 antibody

MARK2 Mouse IgG2a 3C5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MARK2 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with MARK2 polyclonal antibody ( Cat # PAB3195 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu, Ms E, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

MARK2 antibody

MARK2 polyclonal antibody ( Cat # PAB7578 ) ( 0.3 µg/ml ) staining of human brain ( cerebellum ) lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

MARK2 antibody

Western blot using MARK2 (phospho T595) polyclonal antibody ( Cat # PAB10332 ) shows detection of a band at ~82 kDa corresponding to phosphorylated MARK2 ( arrowhead ).
HEK293 cells were transfected with pCMV-3 x Flag-MARK2 plus pCMV-Flag-PKC-zeta ( lane 3 ) or pCMV-3 x Flag-MARK2 only ( lane 4 ).
Total cell lysates were run in a 4-12% Nupage SDS-gel and probed with MARK2 (phospho T595) polyclonal antibody ( Cat # PAB10332 ).
An untransfected HEK293 cell lysate was used as a negative control ( lane 2 ).
MW markers are shown for size comparison ( lane 1 ).
PKC-zeta appears to be involved in the phosphorylation of T595 on MARK2 as increased amounts of phospho- specific staining are observed in lysates from cells transfected with both MARK2 and PKC-zeta.
Personnel Communication, S. Shaw, NCI, Center for Cancer Research. Rabbit Bov, Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

MARK2 antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using MARK2 (Ab-596) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P
50 µg / €205.00
  Assay Biotech

MARK2 antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using MARK2 (Ab-596) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P
0.1 mg / €310.00
  Assay Biotech

MARK2 antibody

Immunofluorescence analysis of A549 cells, using MARK2 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

MARK2 antibody

Immunofluorescence analysis of A549 cells, using MARK2 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €310.00
  Assay Biotech

MARK2 antibody

NBP1-00214 (0.3ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

MARK2 antibody

MARK2 Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

MARK2 antibody

MARK2 Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
50 µg / €290.00
  Novus Biologicals Inc.

MARK2 antibody

MARK2 Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, P
0.1 mg / €360.00
  Novus Biologicals Inc.

MARK2 antibody

MARK2 Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, P
50 µg / €290.00
  Novus Biologicals Inc.

MARK2 antibody

Western Blot: MARK2 Antibody [NBP1-71889]-Whole cell lysate (1 mg for IP, 20% of IP loaded) from 293T cells.  Affinity purified rabbit anti-MARK2 antibody used for IP at 6 mcg/mg lysate.  MARK2 was also immunoprecipitated by rabbit anti-MARK2 antibody NBP1-71890, which recognizes a downstream epitope. Rabbit Aff - Purified Hu IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

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