MARK3 antibody

Principal name

MARK3 antibody

Alternative names for MARK3 antibody

CTAK1, C-TAK1, MAP/microtubule affinity-regulating kinase 3, Serine/threonine-protein kinase p78, PAR-1, STK10, Cdc25C-associated protein kinase 1

SwissProt ID

P27448 (Human), Q03141 (Mouse), Q8VHF0 (Rat)

Gene ID

4140 (MARK3), 5341407 (yajB)

Available reactivities

Hu (Human), Ms (Mouse), Chk (Chicken), Rt (Rat), Ze (Zebrafish), African clawed frog, Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Sheep, Mouse

Available applications

Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of MARK3 antibody

MARK3 is a dual-specificity protein kinase that controls entry into mitosis by dephosphorylating Cdc2 on both threonine 14 and tyrosine 15. MARK3 is phosphorylated on serine 216 throughout interphase but not during mitosis. Serine 216 phosphorylation mediates the binding of 14-3-3 protein to MARK3, and MARK3/14-3-3 complexes are present throughout interphase but not during mitosis (1). Kinase suppressor of Ras (KSR) is a conserved component of the Ras pathway that interacts directly with MEK and MAPK. It has been shown that KSR1 translocates from the cytoplasm to the cell surface in response to growth factor treatment and that this process is regulated by MARK3 (2). MARK3 seems to be a positive regulator of the beta-catenin pathway and an inhibitor of the JNK pathway. These findings show that MARK3, a regulator of polarity, is also a modulator of Wnt-beta-catenin signalling, indicating a link between two important developmental pathways (3).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MARK3 antibody

Western blot analysis of extracts from HeLa cells and 293 cells, using MARK3 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MARK3 antibody

Sample Type: Lane1 = 10ug mouse brain extract; Lane 2 = 50ug mouse brain extract; Primary Antibody Dilution: Anti-MARK3 1:2500; Submitted By: Dr. Veronique Dorval, Laval University Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

MARK3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MARK3 (RC205758, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MARK3. Mouse IgG1 OTI2A4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MARK3 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MARK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805764) Mouse IgG1 OTI2G8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MARK3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MARK3 (RC205758, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MARK3. Mouse IgG1 OTI2D3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MARK3 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MARK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805790) Mouse IgG2a OTI2D9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MARK3 antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MARK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805880) Mouse IgG1 OTI3F6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MARK3 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MARK3 mouse monoclonal antibody. (TA805881; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min) Mouse IgG1 OTI3E11 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MARK3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MARK3 (RC205758, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MARK3. Mouse IgG2b OTI3D8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MARK3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MARK3 (RC205758, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MARK3. Mouse IgG1 OTI4A3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MARK3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MARK3 (RC205758, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MARK3. Mouse IgG2b OTI5E5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MARK3 antibody

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG n.a Purified Hu, Ms IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

MARK3 antibody

Western blot analysis using c-tak1 antibody on His-tagged c-tak1 (1), RKO cell lysate (2) and HCT116 cell lysate (3). Sheep IgG Purified Hu IP, WB
0.25 mg / €360.00
  Acris Antibodies GmbH

MARK3 antibody

Western Blot analysis of MARK3 expression in transfected 293T cell line (H00004140-T01) by MARK3 MaxPab polyclonal antibody.

Lane 1: MARK3 transfected lysate(80.19 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

MARK3 antibody

Western Blot analysis of MARK3 expression in transfected 293T cell line (H00004140-T01) by MARK3 MaxPab polyclonal antibody.

Lane 1: MARK3 transfected lysate(80.19 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

MARK3 antibody

Western Blot analysis of MARK3 expression in transfected 293T cell line (H00004140-T02) by MARK3 MaxPab polyclonal antibody.

Lane 1: MARK3 transfected lysate(81.40 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

MARK3 antibody

Western Blot analysis of MARK3 expression in transfected 293T cell line by MARK3 monoclonal antibody (M01), clone 1A10.

Lane 1: MARK3 transfected lysate(81.4 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2b 1A10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MARK3 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with MARK3 polyclonal antibody ( Cat # PAB2052 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu E, P
0.1 mg / €390.00
  Abnova Taiwan Corp.

MARK3 antibody

Western blot analysis using MARK3 polyclonal antibody on His-tagged c-tak1 (lane 1), RKO cell lysate (lane 2) and HCT-116 cell lysate (lane 3). Sheep Hu IP, WB
0.25 mg / €400.00
  Abnova Taiwan Corp.

MARK3 antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using MARK3 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

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