Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Can (Canine), Bov (Bovine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Sh (Sheep)
Mouse, Rabbit, Goat
Paraffin Sections (P), Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP)
Background of Mast Cell Chymase antibody
This gene product is a chymotryptic serine proteinase that belongs to the peptidase family S1. It is expressed in mast cells and thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II. Angiotensin II has been implicated in blood pressure control and in the pathogenesis of hypertension, cardiac hypertrophy, and heart failure. Thus, this gene product is a target for cardiovascular disease therapies. This gene maps to 14q11.2 in a cluster of genes encoding other proteases. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Immunohistochemistry-Paraffin: Mast Cell Chymase Antibody (CC1) [NB100-693] - Formalin fixed paraffin embedded human tonsil stained with Mast Cell Chymase antibody.
Human COLO205; WB Suggested Anti-CMA1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: COLO205 cell lysate. CMA1 is supported by BioGPS gene expression data to be expressed in COLO205.; CMA1 antibody - C-terminal region (ARP59991_P050) in Human COLO205 cells using Western Blot
HEK293 lysate (10µg protein in RIPA buffer) over expressing Human CMA1 with DYKDDDDK tag probed with this antibody (AP31562PU-N)(0.5µg/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with this antibody (AP31562PU-N)(1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.