Mastermind-Like Protein 1 antibody

Principal name

Mastermind-Like Protein 1 antibody

Alternative names for Mastermind-Like Protein 1 antibody

MAML1, Mam-1

SwissProt ID

Q6T264 (Mouse), Q92585 (Human)

Gene ID

9794 (MAML1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of Mastermind-Like Protein 1 antibody

**Please note, this product is one of a range of Investigative Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot (see Application Notes below). However no additional claims are made for their ability to recognize native protein in any application.

This protein is a homolog of mastermind, a Drosophila protein that plays a role in the Notch signaling pathway involved in cell-fate determination. There is in vitro evidence that the human homolog forms a complex with the intracellular portion of human Notch receptors and can increase expression of a Notch-induced gene. This evidence supports its proposed function as a transcriptional co-activator in the Notch signaling pathway.

General readings

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Lindberg, M.J., et al. FASEB J. 24(7):2396-2404(2010)
Hao, L., et al. Oncogene 29(2):201-213(2010)
Saint Just Ribeiro, M., et al. Curr. Protein Pept. Sci. 10(6):570-576(2009)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Mastermind-Like Protein 1 (N-term) antibody

TA305818 (0.1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Mastermind-Like Protein 1 (Center) antibody

Western blot analysis of Mastermind-Like Protein 1 Antibody (Center) Cat.-No AP52591PU-N in 293,NCI-H460 cell line lysates (35ug/lane). Rabbit Ig Aff - Purified Hu F, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Mastermind-Like Protein 1 antibody

Mastermind-Like Protein 1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Mastermind-Like Protein 1 antibody

Mastermind-Like Protein 1 Goat Hu E
0.1 mg / €400.00
  Abnova Taiwan Corp.

Mastermind-Like Protein 1 antibody

Immunohistochemistry-Paraffin: MAML1 Antibody [NB100-1306] - 3.8ug/ml staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat IgG Aff - Purified Hu E, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Mastermind-Like Protein 1 antibody

Immunofluorescence: MAML1 Antibody [NBP1-33638] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MAML1 (NBP1-33638) antibody at 1:200 dilution. Rabbit IgG Hu ICC/IF, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Mastermind-Like Protein 1 antibody

Immunohistochemistry-Paraffin: MAML1 Antibody [NBP1-83025] - Immunohistochemical staining of human esophagus shows strong nuclear positivity in squamous epithelial cells. Rabbit IgG Aff - Purified Hu ICC/IF, P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Mastermind-Like Protein 1

Mastermind-Like Protein 1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Mastermind-Like Protein 1

Mastermind-Like Protein 1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Mastermind-Like Protein 1

Mastermind-Like Protein 1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

MAML1 overexpression lysate

MAML1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

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