Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Rt (Rat)
Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C)
Background of Mastermind-like protein 3 antibody
The three MAML genes are widely expressed in adult tissues but exhibit distinct expression patterns in mouse early spinal cord development. All MAML proteins localize to nuclear bodies, share a conserved basic domain in their N termini that binds to the ankyrin repeat domain of Notch, and contain a transcriptional activation domain in their C termini. MAML3 acts as a transcriptional coactivator for NOTCH proteins and has been shown to amplify NOTCH-induced transcription of HES1.
Western Blot and IP: MAML3 Antibody [NB100-2128] - Detection of Human and Mouse MAML3 on HeLa whole cell lysate using NB100-2128. MAML3 was also immunoprecipitated by rabbit anti-MAML3 antibodies NB100-2129 and Another MAML3 Antibody.
Western Blot and Immunoprecipitation. Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. Affinity purified rabbit anti-MAML3 antibody NB100-2129. MAML3 was also immunoprecipitated by rabbit anti-MAML3 NB100-2128.
MAML3 Antibody (C-term) (GTX81755) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymphnode followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MAML3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
MAML3 Antibody (C-term) (GTX81755) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.