MAX antibody

Principal name

MAX antibody

Alternative names for MAX antibody

Myc-associated factor X

SwissProt ID

P28574 (Mouse), P52162 (Chick), P52164 (Rat), P61244 (Human)

Gene ID

4149 (MAX)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), Por (Porcine), Ye (Yeast)

Available hosts

Goat, Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of MAX antibody

MAX is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation.The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Multiple alternatively spliced transcript variants have been described for this gene but the full length nature for some of them is unknown.The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Multiple alternatively spliced transcript variants have been described for this gene but the full-length nature for some of them is unknown.

General readings

1. Blackwood, E., Eisenman, R. N. Max: a helix-loop-helix zipper protein that forms a
sequence-specific DNA-binding complex with Myc.Science 251: 1211-1217, 1991.
2. Gilladoga, A. D., Edelhoff, S., Blackwood, E. M., Eisenman, R. N., Disteche, C. M. Mapping
of MAX to human chromosome 14 and mouse chromosome 12 by in situ hybridization. Oncogene 7:
1249-1251, 1992.
3. Hopewell, R., Ziff, E. B. The nerve growth factor-responsive PC12 cell line does not express the
Myc dimerization partner Max. Molec. Cell Biol. 15: 3470-3478, 1995.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MAX (C-term) antibody

TA302711 staining (0.1µg/ml) of Jurkat lysate (RIPA buffer, 1.4E+05 cells per lane).  Primary incubated for 12 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt WB
20 µg / €200.00
  Acris Antibodies GmbH

MAX (C-term) antibody

TA302711 staining (0.1µg/ml) of Jurkat lysate (RIPA buffer, 1.4E+05 cells per lane).  Primary incubated for 12 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAX antibody

Predicted band size: 18 kDa . Positive control: HEK-293 cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MAX antibody

WB Suggested Anti-MAX Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: COLO205 cell lysateThere is BioGPS gene expression data showing that MAX is expressed in COLO205 Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

MAX antibody

WB Suggested Anti-MAX Antibody Titration: 1.25 ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MAX antibody

WB Suggested Anti-MAX Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Lung Rabbit IgG Purified Hu, Ms, Por, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

MAX antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between MAX and SMAD3 HeLa cells were stained with anti-MAX rabbit purified polyclonal 1:1200 and anti-SMAD3 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

MAX antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between MAPK14 and MAX HeLa cells were stained with anti-MAPK14 rabbit purified polyclonal 1:1200 and anti-MAX mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2a 4E10-1A9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MAX antibody

MAX polyclonal antibody ( Cat # PAB6023 ) staining ( 0.1 µg/ml ) of Jurkat lysate ( RIPA buffer, 1.4E+05 cells per lane ) . Primary incubated for 12 hour. Detected by western blot using chemiluminescence. Goat Hu, Ms, Rt E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

MAX antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MAX (Ab-2) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
50 µg / €205.00
  Assay Biotech

MAX antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MAX (Ab-2) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
0.1 mg / €310.00
  Assay Biotech

MAX antibody

Human HepG2; WB Suggested Anti-MAX Antibody Titration: 1.25ug/ml. Positive Control: HepG2 cell lysate; MAX antibody - middle region (ARP38145_T100) in Human HepG2 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

MAX antibody

Human Lung; WB Suggested Anti-MAX Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Lung; MAX antibody - N-terminal region (ARP39844_P050) in Human Lung cells using Western Blot Rabbit Aff - Purified African clawed frog, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

MAX antibody

Human COLO205; WB Suggested Anti-MAX Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: COLO205 cell lysate. There is BioGPS gene expression data showing that MAX is expressed in COLO205.; MAX antibody - N-terminal region (P100953_P050) in Human COLO205 cells using Western Blot Rabbit Aff - Purified African clawed frog, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

MAX antibody

MAX Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

MAX pSer11 antibody

Western blot analysis of MAX (pS11) expression in Hela (A); mouse heart (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt WB
30 µl / €210.00
  Cohesion Biosciences Ltd.

MAX pSer11 antibody

Western blot analysis of MAX (pS11) expression in Hela (A); mouse heart (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt WB
0.1 ml / €330.00
  Cohesion Biosciences Ltd.

MAX pSer11 antibody

Western blot analysis of MAX (pS11) expression in Hela (A); mouse heart (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt WB
0.2 ml / €530.00
  Cohesion Biosciences Ltd.

MAX antibody

Western blot:MAX Antibody [NB 100-793]- staining (0.1ug/ml) of Jurkat lysate (RIPA buffer, 1.4E+05 cells per lane). Goat IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

MAX antibody

Western Blot: MAX Antibody [NBP1-49963] - Detection of Human MAX by Western Blot and Immunoprecipitation.  Samples:  Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells.  Antibodies: Affinity purified rabbit anti-MAX antibody used for WB at 0.1 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate.  For blotting immunoprecipitated MAX was used.  Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B). Rabbit Aff - Purified Hu IP, P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

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