MBD1 antibody

Principal name

MBD1 antibody

Alternative names for MBD1 antibody

Methyl-CpG-binding domain protein 1, CXXC3, PCM1, Protein containing methyl-CpG-binding domain 1

SwissProt ID

Q9UIS9 (Human), Q9Z2E2 (Mouse)

Gene ID

4152 (MBD1)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), ChIP assay (ChIP)

Background of MBD1 antibody

MBD1 belongs to a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD1 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters; in addition, variants with three CXXC domains also repress unmethylated promoter activity. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters; in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21.DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters; in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21.

General readings

Villa,R., et al., (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (5), 1400-1405

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MBD1 antibody

Western blot analysis of MBD1 using Jurkat cell lysate. Rabbit Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MBD1 antibody

WB Suggested Anti-MBD1 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

MBD1 antibody

Western Blot: MBD1 Antibody (100B272.1) [TA336395] - analysis of Jurkat cell lysate using anti-MBD1 antibody. Image from verified customer review. Mouse IgG1 100B272.1 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MBD1 antibody

WB Suggested Anti-MBD1 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 312500; Positive Control: Jurkat cell lysate.MBD1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MBD1 antibody

WB Suggested Anti-MBD1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Can, Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

MBD1 antibody

WB using the antibody against MBD1 diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right, the marker (in kDa) is shown on the left. Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

MBD1 antibody

Immunofluorescence of purified MaxPab antibody to MBD1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

MBD1 antibody

Immunofluorescence of purified MaxPab antibody to MBD1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

MBD1 antibody

Western Blot analysis of MBD1 expression in transfected 293T cell line (H00004152-T01) by MBD1 MaxPab polyclonal antibody.

Lane 1: MBD1 transfected lysate(66.60 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

MBD1 antibody

Immunoperoxidase of monoclonal antibody to MBD1 on formalin-fixed paraffin-embedded human lung. [antibody concentration 3 ug/ml] Mouse IgG2b 2C7 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MBD1 antibody

MBD1 monoclonal antibody (M03), clone 4F6. Western Blot analysis of MBD1 expression in IMR-32 ( Cat # L008V1 ). Mouse IgG2b 4F6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MBD1 antibody

MBD1 monoclonal antibody (M04), clone 2H3. Western Blot analysis of MBD1 expression in Jurkat ( Cat # L017V1 ). Mouse IgG2b 2H3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MBD1 antibody

MBD1 monoclonal antibody (M05), clone 2B10. Western Blot analysis of MBD1 expression in human kidney. Mouse IgG2b 2B10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MBD1 antibody

MBD1 Mouse IgG2b 2B10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MBD1 antibody

Western blot analysis of MBD1 in HeLa cell lysate with MBD1 polyclonal antibody ( Cat # PAB0172 ) . A protein band with an approximate molecular weight of 55 kDa was detected. Rabbit Hu WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

MBD1 antibody

Human osteosarcoma cells ( U-2 OS ) were transfected with an expression vector for TY1-tagged MBD1.
The presence of the TY1-MBD1 in the cell lysates was demonstrated by western blot analysis with the antibody directed against the TY1-tag and with MBD1 polyclonal antibody ( Cat # PAB14113 ) ( lane 2 ), diluted 1 : 2,000 in TBST containing 3% milk powder. Rabbit Hu E, WB
50 µg / €390.00
  Abnova Taiwan Corp.

MBD1 antibody

ELISA was performed using a serial dilution of MBD1 polyclonal antibody ( Cat # PAB14126 ), crude serum and Flow Through in antigen coated wells.
By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 16,500. Rabbit Hu ChIP, E
50 µg / €470.00
  Abnova Taiwan Corp.

MBD1 antibody

Human HepG2; WB Suggested Anti-MBD1 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; MBD1 antibody - C-terminal region (ARP32012_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

MBD1 antibody

Human Jurkat; WB Suggested Anti-MBD1 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate. MBD1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; MBD1 antibody - C-terminal region (ARP40168_T100) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

MBD1 antibody

Human HepG2; WB Suggested Anti-MBD1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; MBD1 antibody - middle region (ARP40172_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

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