MBD1 antibody

Principal name

MBD1 antibody

Alternative names for MBD1 antibody

Methyl-CpG-binding domain protein 1, CXXC3, PCM1, Protein containing methyl-CpG-binding domain 1

SwissProt ID

Q9UIS9 (Human), Q9Z2E2 (Mouse)

Gene ID

4152 (MBD1), 17190 (Mbd1)

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), ChIP assay (ChIP)

Background of MBD1 antibody

MBD1 belongs to a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD1 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters; in addition, variants with three CXXC domains also repress unmethylated promoter activity. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters; in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21.DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters; in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21.

General readings

Villa,R., et al., (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (5), 1400-1405

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MBD1 antibody

  Rabbit polyclonal anti-MBD1 antibody  
Western blot analysis of MBD1 using Jurkat cell lysate. Rabbit Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MBD1 antibody

  Rabbit Polyclonal Anti-MBD1 Antibody  
WB Suggested Anti-MBD1 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

MBD1 antibody

  Mouse Monoclonal MBD1 Antibody (100B272.1)  
Western Blot: MBD1 Antibody (100B272.1) [TA336395] - analysis of Jurkat cell lysate using anti-MBD1 antibody. Image from verified customer review. Mouse IgG1 100B272.1 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MBD1 antibody

  Rabbit Polyclonal Anti-MBD1 Antibody  
WB Suggested Anti-MBD1 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 312500; Positive Control: Jurkat cell lysate.MBD1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MBD1 antibody

  Rabbit Polyclonal Anti-MBD1 Antibody  
WB Suggested Anti-MBD1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Can, Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

MBD1 antibody

  Rabbit Polyclonal MBD1 Antibody  
WB using the antibody against MBD1 diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right, the marker (in kDa) is shown on the left. Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

MBD1 - human gene knockout kit via CRISPR

MBD1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

MBD1 - human gene knockout kit via CRISPR

MBD1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

MBD1 - human gene knockout kit via CRISPR

MBD1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

MBD1 - human gene knockout kit via CRISPR

MBD1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Mbd1 - mouse gene knockout kit via CRISPR

Mbd1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Mbd1 - mouse gene knockout kit via CRISPR

Mbd1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Mbd1 - mouse gene knockout kit via CRISPR

Mbd1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Mbd1 - mouse gene knockout kit via CRISPR

Mbd1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

MBD1 overexpression lysate

MBD1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

MBD1 overexpression lysate

MBD1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

MBD1 overexpression lysate

MBD1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

MBD1 overexpression lysate

MBD1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Lysates (Knockout)

Catalog No.    

MBD1-knockout Cell lysate

MBD1-knockout Cell lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, MBD1 (mGFP-tagged)-Human methyl-CpG binding domain protein 1 (MBD1), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, MBD1 (mGFP-tagged)-Human methyl-CpG binding domain protein 1 (MBD1), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,230.00
  OriGene Technologies, Inc.

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