MBD2 antibody

Principal name

MBD2 antibody

Alternative names for MBD2 antibody

Methyl-CpG-binding domain protein 2, Demethylase, DMTase

SwissProt ID

Q9UBB5 (Human), Q9Z2E1 (Mouse)

Gene ID

8932 (MBD2), 17191 (Mbd2)

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Rt (Rat), Bov (Bovine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Electrophoretic Mobility Shift Assay (EMSA), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C)

Background of MBD2 antibody

MBD2 belongs to a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated D. MBD2 can also repress transcription from methylated gene promoters. MBD2 may function as a mediator of the biological consequences of the methylation sigl.It is also reported that the MBD2 functions as a demethylase to activate transcription, as D methylation causes gene silencing.D methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated D. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation sigl. It is also reported that the this protein functions as a demethylase to activate transcription, as D methylation causes gene silencing. D methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated D. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation sigl. It is also reported that the this protein functions as a demethylase to activate transcription, as D methylation causes gene silencing.

General readings

Auriol E, Billard LM, Magdinier F, Dante R. Specific binding of the methyl binding domain protein 2 at the BRCA1-NBR2 locus. Nucleic Acids Res. 2005 Jul 28;33(13):4243-54. Print 2005. PMID: 16052033

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MBD2 (15-29) antibody

Human Skin (formalin-fixed, paraffin-embedded) stained with MBD2 antibody AP22575PU-N followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat Aff - Purified Bear, Bov, Bt, Can, Elephant, Eq, Hst, Hu, Mky, Ms, Op, Por, Prim, Rb, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

MBD2 antibody

Immunohistochemistry-Paraffin: MBD2 Antibody [NB100-81656] - human breast carcinoma  Affinity purified rabbit anti-MBD2 used at a dilution of 1:100.  Detection: Red-fluorescent goat anti-rabbit IgG highly crossadsorbed Antibody used at a dilution of 1:100. Rabbit IgG Aff - Purified Hu ICC/IF, IP, P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

MBD2 (isoform 1) antibody

TA303172 (0.3µg/ml) staining of Jurkat cell lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MBD2 antibody

Western blot analysis of MBD2a using Jurkat cell lysate. Rabbit Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MBD2 antibody

MBD2 Antibody (Center) (Cat. #TA324684) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the MBD2  antibody detected the MBD2  protein (arrow). Rabbit IgG Purified Hu, Ms ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

MBD2 antibody

Lanes: ; Lane 1: 15ug WT mouse ES lysate; Lane 2: 15ug MBD2 KO mouse ES lysate; Primary Antibody Dilution: ; 1:1000; Secondary Antibody: ; Goat anti-rabbit-HRP; Secondary Antibody Dilution: ; 1:2500; Gene Name: ; MBD2 a; Submitted by: ; Austin J. Cooney, Rabbit IgG Purified Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

MBD2 antibody

Lanes: Lane 1: 15ug WT mouse ES lysate; Lane 2: 15ug MBD2 KO mouse ES lysate; Primary Antibody Dilution: 1: 1000; Secondary Antibody: Goat anti-rabbit-HRP; Secondary Antibody Dilution: 1: 2500; Gene Name: MBD2 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MBD2 antibody

WB was performed on nuclear extracts from mouse fibroblastst (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse MBD2, diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest is indicated on the right. Rabbit IgG Purified Ms E
0.1 ml / €325.00
  OriGene Technologies, Inc.

MBD2 (N-term) antibody

Western Blot analysis of anti-MBD2 N-term Antibody (Cat.#AP11082PU-N) to detect MBD2 in A375 cell lysate (lane 1) and mouse brain tissue lysate (lane 2) lysate. Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MBD2 antibody

Kidney stained in IHC-P with MBD2 antibody Rabbit Purified Hu C, E, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

MBD2 antibody

The MBD2 polyclonal antibody ( Cat # PAB2234 ) is used in Western blot to detect MBD2 in A-375 cell lysate ( lane 1 ) and mouse brain tissue lysate ( lane 2 ) lysate. Rabbit Hu, Ms E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

MBD2 antibody

MBD2 polyclonal antibody ( Cat # PAB7163 ) ( 0.3 µg/ml ) staining of Jurkat cell lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

MBD2 antibody

Western blot was performed on nuclear extracts from mouse fibroblastst ( NIH/3T3 ) and embryonic stem cells ( E14Tg2a ) with Mbd2 polyclonal antibody ( Cat # PAB14065 ), diluted 1 : 500 in BSA/PBS-Tween. Rabbit Ms E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

MBD2 antibody

Mouse ES; . Lanes: Lane 1: 15ug WT mouse ES lysate. Lane 2: 15ug MBD2 KO mouse ES lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Goat anti-rabbit-HRP. Secondary Antibody Dilution: 1:2500. Gene Name: MBD2 a. Submitted by: Austin J. Cooney, Baylor College of Medicine.; MBD2 antibody - N-terminal region (ARP32485_P050) in Mouse ES cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

MBD2 antibody

Mouse ES; . Lanes: Lane 1: 15ug WT mouse ES lysate. Lane 2: 15ug MBD2 KO mouse ES lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Goat anti-rabbit-HRP. Secondary Antibody Dilution: 1:2500. Gene Name: MBD2 a. Submitted by: Austin J. Cooney, Baylor College of Medicine.; MBD2 antibody - middle region (ARP40169_T100) in Mouse ES cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

MBD2 (Isoform 1) antibody

Anti-MBD2a antibody produced in rabbit Rabbit Serum Hu, Ms EMSA, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

MBD2 antibody

Immunohistochemistry-Paraffin: MBD2 Antibody [NB100-55415] - (2.5µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

MBD2 antibody

Western Blot and  Immunoprecipitation: MBD2 Antibody [NB100-81657] - Detection of Human and Mouse MBD2 on HeLa whole cell lysate using NB100-81657. MBD2 was also immunoprecipitated by rabbit anti-MBD2 antibody NB100-81656. Rabbit IgG Aff - Purified Hu, Ms IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Assay Kits

Catalog No.    

MBD2 Binding Activity/Inhibition Assay Kit

MBD2 Binding Activity/Inhibition Assay Kit  
1 kit / €590.00
  Abnova Taiwan Corp.

19 Item(s)

per page
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