MBD3 antibody

Principal name

MBD3 antibody

Alternative names for MBD3 antibody

Methyl-CpG-binding domain protein 3

SwissProt ID

O95983 (Human), Q9Z2D8 (Mouse)

Gene ID

53615 (MBD3), 17192 (Mbd3)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Rt (Rat), Eq (Equine), GP (Guinea Pig)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C)

Background of MBD3 antibody

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex.DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Nan X., et al. (1998) Nature. 393:386-389Hendrich B., et al. (1999) Mamm Genome. 10: 906-912.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MBD3 antibody

Western blot analysis of extracts from Jurkat cells, using MBD3 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MBD3 antibody

MBD3 Antibody (C-term) (Cat. #TA325172) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the MBD3 antibody detected the MBD3 protein (arrow). Mouse IgG1 995CT3.2.2 Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

MBD3 antibody

Western Blot: MBD3/MBD2 Antibody (106B691) [TA336393] - Analysis of MBD2 and MBD3 using this antibody. HeLa cell lysate (nuclear fraction) probed with MBD3/2 antibody at 2 ug/ml in the A) absence and B) presence of immunizing peptide. Mouse IgG1 106B691 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MBD3 antibody

WB Suggested Anti-MBD3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

MBD3 antibody

WB Suggested Anti-Mbd3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Mouse Heart Rabbit IgG Purified Can, GP, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

MBD3 antibody

WB on nuclear extracts from a Human leukemic monocyte lymphoma cell line (U937 NE, 60 ug) with the ab at 1:2,000 in TBS-Tween with 5% milk. B: TY1-tagged MBD3 (TTE-MBD3) was over-expressed in osteosarcoma cells (U2OS). Nuclear extracts were prepared and separated by electrophoresis. An ab against the TY1 tag or the ab against MBD3 (1:2,000 in TBS-Tween & 5% milk) were used to detect TTE-MBD3 (lane 1 and 2, respectively). Rabbit IgG Purified Hu E
0.1 ml / €325.00
  OriGene Technologies, Inc.

MBD3 antibody

Western blot analysis on Jurkat cell lysate using MBD3 Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MBD3 (215-230) antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Mouse IgG1 106B691 Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, Fi, Hst, Hu, Mky, Ms, Op, Platypus, Por, Prim, Rb, Tr, Xen P, WB
50 µg / €470.00
  Acris Antibodies GmbH

MBD3 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MBD3 antibody

Western blot (WB) analysis of MBD3 antibody (Cat.-No.: AP20404PU-N) in extracts from Jurkat cells. Rabbit Aff - Purified Hu, Ms WB
0.1 mg / €350.00
  Acris Antibodies GmbH

MBD3 antibody

MBD3 polyclonal antibody (A01), Lot # 060509JCS1 Western Blot analysis of MBD3 expression in Y-79 ( Cat # L042V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

MBD3 antibody

Western Blot analysis of MBD3 expression in transfected 293T cell line (H00053615-T01) by MBD3 MaxPab polyclonal antibody.

Lane 1: MBD3 transfected lysate(32.01 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

MBD3 antibody

Immunoprecipitation of MBD3 transfected lysate using anti-MBD3 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with MBD3 MaxPab mouse polyclonal antibody (B01) (H00053615-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

MBD3 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the MBD3 polyclonal antibody ( Cat # PAB2214 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu, Ms E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

MBD3 antibody

Western blot was performed on nuclear extracts from a Human leukemic monocyte lymphoma cell line ( U-937 NE, 60 µg ) with MBD3 polyclonal antibody ( Cat # PAB14055 ), diluted 1 : 2,000 in TBS-Tween containing 5% skimmed milk. Rabbit Hu E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

MBD3 antibody

Western blot analysis of extracts from Jurkat cells, using MBD3 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €205.00
  Assay Biotech

MBD3 antibody

Western blot analysis of extracts from Jurkat cells, using MBD3 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €310.00
  Assay Biotech

MBD3 antibody

Transfected 293T; WB Suggested Anti-MBD3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; MBD3 antibody - N-terminal region (ARP40170_P050) in Transfected 293T cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

MBD3 (N-term) antibody

MBD3 Rabbit Aff - Purified Hu, Ms WB
50 µg / €350.00
  AVIVA Systems Biology

MBD3 antibody

Western Blot: MBD3 Antibody [NBP1-19559] - extracts from Jurkat cells. Rabbit Aff - Purified Hu, Ms WB
0.1 mg / €420.00
  Novus Biologicals Inc.

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