MBNL1 antibody

Principal name

MBNL1 antibody

Alternative names for MBNL1 antibody

Muscleblind-like protein 1, EXP

SwissProt ID

Q5ZKW9 (Chick), Q9JKP5 (Mouse), Q9NR56 (Human)

Gene ID

4154 (MBNL1)

Available reactivities

Hu (Human), Can (Canine), Chk (Chicken), Ms (Mouse), Ze (Zebrafish), Bov (Bovine), Rt (Rat), Eq (Equine), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C)

Background of MBNL1 antibody

pre-mRs splicing /removal of non-coding regions is a critical process which contributes to a diverse proteome as well as gene expression regulation, and there are several splicing factors that are exclusively involved in a subset of splicing decisions - human muscleblind-like family of R-binding proteins: MBNL1/2/3 also known as MBNL/EXP, MBLL/MPL1, and MBXL/CHCR, respectively. Muscleblind-like 1 (MBNL1) is a known mediator of pre-mR altertive splicing regulation and it acts either as activator or repressor of splicing on specific pre-mR targets. Several pathologies are linked to expansion of polynucleotide sequences (mostly trinucleotide repeats) and CUG/CCUG expansion Rs accumulates in Myotonic Dystrophy patient cells wherein it contributes to the disease by dysregulating altertive splicing factors such as CUGBP, Elav-like Family Member 1 (CELF1) and MBNL1. The expanded CUG repeats sequester MBNL proteins into nuclear foci, leading to loss of active protein and the sequestration of MBNL1 leads to missplicing of developmentally regulated events that are responsible for symptoms in DM types 1 and 2, such as the missplicing of the chloride channel (CLCN1) leading to myotonia. CELF1 and MBNL1 regulate a number of common splicing events, but in opposite directions and loss of MBNL1 activity is more critical than upregulation of CELF1 activity for abnormal splicing.

General readings

Warf MB, Berglund JA.
MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T.
RNA. 2007 Dec;13(12):2238-51.
PMID: 17942744

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MBNL1 antibody

Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 3E7 Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

MBNL1 (C-term) antibody

  Goat Anti-MBNL1 Antibody  
TA305699 (1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MBNL1 antibody

  Mouse Monoclonal Muscleblind-like 1 Antibody (HL-1822 3A4-1E9)  
Western Blot: Muscleblind-like 1 Antibody (HL-1822 3A4-1E9) [TA336512] - Detection of Muscleblind in HeLa whole cell extract (33ug) using NB 110-37256. Mouse IgG1 HL-1822 3A4-1E9 Purified Hu, Ms, Rt C, ICC/IF, P, WB
0.5 ml / €325.00
  OriGene Technologies, Inc.

MBNL1 antibody

  Rabbit Polyclonal Anti-MBNL1 Antibody  
WB Suggested Anti-MBNL1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Jurkat cell lysate; MBNL1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Can, Eq, Hu, Ms, Por, Rb, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

MBNL1 antibody

  Rabbit Polyclonal Anti-MBNL1 Antibody  
WB Suggested Anti-MBNL1 Antibody Titration: 2.5 ug/ml; ELISA Titer: 1: 62500; Positive Control: Jurkat cell lysateMBNL1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Hu, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

MBNL1 - human gene knockout kit via CRISPR

MBNL1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

MBNL1 - human gene knockout kit via CRISPR

MBNL1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

MBNL1 - human gene knockout kit via CRISPR

MBNL1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

MBNL1 - human gene knockout kit via CRISPR

MBNL1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MBNL1 (transcript variant 6)

  Recombinant protein of human muscleblind-like (Drosophila) (MBNL1), transcript variant 6  
MBNL1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

MBNL1 (transcript variant 1)

  Recombinant protein of human muscleblind-like (Drosophila) (MBNL1), transcript variant 1  
MBNL1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

MBNL1 (transcript variant 5)

  Recombinant protein of human muscleblind-like (Drosophila) (MBNL1), transcript variant 5  
MBNL1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

MBNL1 (transcript variant 3)

  Purified recombinant protein of Homo sapiens muscleblind-like (Drosophila) (MBNL1), transcript variant 3  
MBNL1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

MBNL1 (transcript variant 7)

  Purified recombinant protein of Homo sapiens muscleblind-like (Drosophila) (MBNL1), transcript variant 7  
MBNL1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

MBNL1 overexpression lysate

MBNL1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

MBNL1 overexpression lysate

MBNL1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

MBNL1 overexpression lysate

MBNL1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

MBNL1 overexpression lysate

MBNL1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

MBNL1 overexpression lysate

MBNL1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

MBNL1 overexpression lysate

MBNL1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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