MCEE antibody

Principal name

MCEE antibody

Alternative names for MCEE antibody

mitochondrial Methylmalonyl-CoA epimerase, DL-methylmalonyl-CoA racemase

SwissProt ID

Q2KIZ3 (Bovin), Q96PE7 (Human), Q9D1I5 (Mouse)

Gene ID

84693 (MCEE)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of MCEE antibody

MCEE catalyzes the interconversion of D- and L-methylmalonyl-CoA during the degradation of branched chain amino acids, odd chain-length fatty acids, and other metabolites. This protein deficiency is an autosomal recessive inborn error of amino acid metabolism, involving valine, threonine, isoleucine and methionine. This organic aciduria may present in the neonatal period with life-threatening metabolic acidosis, hyperammonemia, feeding difficulties, pancytopenia and coma. Recombinant human MCEE protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

General readings

Yang GE., et al. (2009) Appl Biochem Biotechnol. 152(3):353-65.
Gradinger AB., et al. (2007) Hum Mutat. 28(10):1045.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MCEE antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MCEE mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808532)(1:150) Mouse IgG2b OTI1B5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MCEE antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MCEE mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808534)(1:150) Mouse IgG2a OTI6A1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MCEE antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MCEE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808608)(1:150) Mouse IgG1 OTI11G7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MCEE antibody

WB Suggested Anti-MCEE Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human brain Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

MCEE antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCEE (RC205018, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCEE.(1:2000) Mouse IgG1 OTI9E12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MCEE antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCEE (RC205018, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCEE.(1:2000) Mouse IgG1 OTI4A6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MCEE (C-term) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma reacted with MCEE Antibody (C-term) Cat.-No AP52633PU-N, which peroxidase conjugated to the secondary antibody and followed by  DAB staining. Rabbit Ig Aff - Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

MCEE antibody

Western Blot analysis of MCEE expression in transfected 293T cell line (H00084693-T01) by MCEE MaxPab polyclonal antibody.

Lane 1: MCEE transfected lysate(19.36 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

MCEE antibody

MCEE Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

MCEE (C-term) antibody

Human Brain; WB Suggested Anti-MCEE Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human brain; MCEE antibody - C-terminal region (ARP60330_P050) in Human Brain cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

MCEE antibody

MCEE Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

MCEE antibody

Immunohistochemistry-Paraffin: MCEE Antibody [NBP1-81823] - Immunohistochemical staining of human placenta shows strong cytoplasmic and membranous positivity in trophoblastic cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MCEE (full length, N-term HIS tag)

MCEE Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

MCEE (37-176, His-tag)

MCEE Human Purified > 90 % E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

MCEE (37-176, His-tag)

MCEE Human Purified > 90 % E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

MCEE

MCEE Human Purified
2 µg / €300.00
  Abnova Taiwan Corp.

MCEE

MCEE Human Purified
0.1 mg / €380.00
  Novus Biologicals Inc.

MCEE

MCEE Human Purified
0.5 mg / €870.00
  Novus Biologicals Inc.

Lysates

Catalog No.    

MCEE 293T Cell Transient Overexpression Lysate(Denatured)

MCEE 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

MCEE overexpression lysate

MCEE overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

20 Item(s)

per page
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