MCL1 antibody

Principal name

MCL1 antibody

Alternative names for MCL1 antibody

BCL2L3, Bcl-2-like protein 3, mcl1/EAT, EAT, r004, Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-related protein

SwissProt ID

P97287 (Mouse), Q07820 (Human), Q9Z1P3 (Rat)

Gene ID

4170 (MCL1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Fe (Feline), Mky (Monkey), Por (Porcine), Rb (Rabbit), Bov (Bovine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Flow Cytometry (F)

Background of MCL1 antibody

Mcl-1 is an anti-apoptotic member of the Bcl-2 family origilly isolated from the ML-1 human myeloid leukemia cell line during phorbol ester-induced differentiation along the monocyte/macrophage pathway. Similar to other Bcl-2 family members, Mcl-1 localizes to the mitochondria, interacts with and antagonizes pro-apoptotic Bcl-2 family members, and inhibits apoptosis induced by a number of cytotoxic stimuli. Mcl-1 differs from its other family members in its regulation at both the transcriptiol and post-translatiol level. First, Mcl-1 has an extended amino-termil PEST region, which is responsible for its relatively short half-life. Second, unlike other family members, Mcl-1 is rapidly transcribed via a PI3K/Akt dependent pathway, resulting in its increased expression during myeloid differentiation and cytokine stimulation. Mcl-1 is phosphorylated in response to treatment with phorbol ester, microtubule-damaging agents, oxidative stress, and cytokine withdrawal . Phosphorylation at Thr163, the conserved MAP kise/ERK site located within the PEST region, slows Mcl-1 protein turnover but may prime the GSK-3 mediated phosphorylation at Ser159 that leads to Mcl-1 destabilization. Mcl-1 deficiency in mice results in peri-implantation lethality. In addition, conditiol disruption of the corresponding mcl-1 gene shows that Mcl-1 plays an important role in early lymphoid development and in the maintence of mature lymphocytes.

General readings

1. Kozopas, K.M. et al. (1993) MCL1, a gene expressed in programmed myeloid cell differentiation, has sequence similarity to BCL2. P.N.A.S USA 90: 3516-3520.
2. Bae, J. et al. (2000) MCL-1S, a splicing variant of the antiapoptotic BCL-2 family member MCL-1, encodes a proapoptotic protein possessing only the BH3 domain.
J. Biol. Chem. 275: 25255-25261.
3. Rinkenberger, J.L. et al. (2000) Mcl-1 deficiency results in peri-implantation embryonic lethality.
Genes and Dev. 14: 23-27.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MCL1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-MCL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500986, Dilution 1:50) Mouse IgG2a OTI2E11 Purified Hu F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

MCL1 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-MCL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501036, Dilution 1:50) Mouse IgG1 OTI10F6 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MCL1 antibody

Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-MCL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500983, Dilution 1:50) Mouse IgG2a OTI3A12 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MCL1 antibody

Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-MCL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500984, Dilution 1:50) Mouse IgG1 OTI4B7 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MCL1 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-MCL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500985, Dilution 1:50) Mouse IgG2a OTI9D7 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MCL1 antibody

Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-MCL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500987, Dilution 1:50) Mouse IgG2b OTI9C9 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MCL1 antibody

Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-MCL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500988, Dilution 1:50) Mouse IgG2a OTI3B4 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MCL1 antibody

Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-MCL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500989, Dilution 1:50) Mouse IgG1 OTI10C5 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MCL1 antibody

Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-MCL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500990, Dilution 1:50) Mouse IgG2a OTI10D10 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MCL1 antibody

Human small intestine stained with anti-Mcl-1 antibody Rabbit Purified Hu, Ms P
0.5 ml / €360.00
  Acris Antibodies GmbH

MCL1 antibody

Human small intestine stained with anti-Mcl-1 antibody Rabbit Purified Hu P
1 ml / €490.00
  Acris Antibodies GmbH

MCL1 antibody

Human small intestine stained with anti-Mcl-1 antibody Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

MCL1 (Isoform 1) (C-term) antibody

TA302945 staining (1µg/ml) of Human Lung lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Can, Fe, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCL1 (C-term) antibody

Western blot analysis of Mcl-1 in Raji cell lysates with Mcl-1 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCL1 (N-term) antibody

Western blot analysis of Mcl-1in Raji cell lysates with Mcl-1 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCL1 (Center) antibody

Western blot analysis of Mcl-1 in Raji cell lysates with Mcl-1 antibody (IN) at (A) 0.5, (B) 1, and (C) 2 ug/mL. Rabbit IgG Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCL1 antibody

Predicted band size: 37 kDa . Positive control: Raji, Jurkat, HL-60 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1,2,3: Raji, Jurkat, HL-60 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MCL1 antibody

Western blot analysis of extracts from HuvEc cells, using MCL1 antibody (#TA326250).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MCL1 antibody

Western blot analysis of extracts of various cell lines, using MCL1 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCL1 antibody

A20 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-Mcl-1 polyclonal antibody. Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Poly6136 Purified Hu, Ms WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

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