MCM2 antibody

Principal name

MCM2 antibody

Alternative names for MCM2 antibody

BM28, CCNL1, CDCL1, Nuclear protein BM28, DNA replication licensing factor MCM2, Minichromosome maintenance protein 2 homolog

SwissProt ID

P29469 (Yeast), P49736 (Human), P97310 (Mouse)

Gene ID

4171 (MCM2), 17216 (Mcm2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Ye (Yeast), Xen (Xenopus), Chk (Chicken), Ze (Zebrafish), African clawed frog, Mky (Monkey), S. cerevisiae (Saccharomyces cerevisiae)

Available hosts

Mouse, Rabbit

Available applications

Frozen Sections (C), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F), ELISA (detection) (E(detection))

Background of MCM2 antibody

The minichromosome maintence (MCM) 2-7 proteins are a family of six related proteins required for the initiation and elongation of D replication. MCM2-7 bind together to form the heterohexameric MCM complex that is thought to act as a replicative helicase at the D replication fork. This complex is also a key component of the pre-replication complex (pre-RC) (reviewed in 1). Cdc6 and CDT1 recruit the MCM complex to the origin recognition complex (ORC) during late mitosis/early G1 phase forming the pre-RC and licensing the D for replication. Phosphorylation of the MCM2, MCM3, MCM4, and MCM6 subunits appears to regulate MCM complex activity and the initiation of D synthesis. MCM proteins are removed during D replication, causing chromatin to become unlicensed through inhibition of pre-RC reformation. Licensing of the chromatin permits the D to replicate only once per cell cycle, thereby helping to ensure that genetic alterations and malignt cell growth do not occur. Studies have shown that the MCM complex is involved in checkpoint control by protecting the structure of the replication fork and assisting in restarting replication by recruiting checkpoint proteins after arrest.

General readings

Saade, E., et al. Proteomics 9(21):4934-4943(2009)
Xu, X., et al. EMBO J. 28(19):3005-3014(2009)
Liaw, K., et al. J. Cutan. Pathol. 36(10):1121-1122(2009)
Im, J.S., et al. Proc. Natl. Acad. Sci. U.S.A. 106(37):15628-15632(2009)
Chuang, L.C., et al. Mol. Cell 35(2):206-216(2009)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MCM2 (21-31) antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Skin. Rabbit Aff - Purified Hu, Ms, Rt, Ye E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

MCM2 antibody

  Rabbit Monoclonal anti-MCM2 Antibody  
Western Blot analysis of HeLa cell lysate with MCM2 antibody Rabbit IgG SP85 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MCM2 antibody

  MCM2 mouse monoclonal antibody, clone OTI1G4 (formerly 1G4)  
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MCM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805472) Mouse IgG2b OTI1G4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MCM2 antibody

  MCM2 mouse monoclonal antibody, clone OTI3C12 (formerly 3C12)  
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MCM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805484) Mouse IgG1 OTI3C12 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MCM2 antibody

  MCM2 mouse monoclonal antibody, clone OTI1G10 (formerly 1G10)  
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MCM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805521) Mouse IgG2b OTI1G10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MCM2 antibody

  MCM2 mouse monoclonal antibody, clone OTI8A11 (formerly 8A11)  
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MCM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805566) Mouse IgG2b OTI8A11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MCM2 antibody

Immunohistochemical analysis of paraffin-embedded colon cancer tissues using MCM2 mouse mAb with DAB staining. Mouse IgG1 2B3 Ascites Hu, Mky, Ms, Rt E, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

MCM2 antibody

Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using MCM2 mouse mAb with DAB staining. Mouse IgG1 1E7 Ascites Hu E, F, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

MCM2 antibody

  Rabbit polyclonal anti-MCM2 antibody  
Western blot analysis of extracts from 293 cells, using MCM2 antibody (#TA312162).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MCM2 (21-31) antibody

  Rabbit polyclonal anti-MCM2 antibody  
WB using anti-MCM2 antibody shows detection of both phosphorylated and unphosphorylated MCM2 in nuclear extracts from elutriated human cells(MO59K/K562). Panel A shows WB results for asynchronous cells lyastes(lane 1), cells arrested in early S with aphidicolin (lane2), and cells arrested in mitosis with nocodazole (lane 3). Panel B shows a schematic diagram of bands representing phosphorylated and unphosphorylated MCM2 present in these preparations. The primary antibody was diluted 1:400. Rabbit IgG Aff - Purified Hu, Ms, Rt, S. cerevisiae E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

MCM2 antibody

  Rabbit Polyclonal MCM2 Antibody  
Western blot analysis on 293 cell lysate using MCM2 Antibody Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MCM2 antibody

  Rabbit anti-MCM2 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using MCM2 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCM2 antibody

  Rabbit Polyclonal anti-MCM2 Antibody  
Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-MCM2 antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system. Rabbit IgG Poly6022 Purified Hu, Ms ICC/IF, WB
50 µl / €325.00
  OriGene Technologies, Inc.

MCM2 antibody

  Rabbit Polyclonal Anti-MCM2 Antibody  
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-5: 293T cells, hela cells, K562 cells, Raji cells, 231 cells, Primary antibody: (MCM2 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 second Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MCM2 antibody

  Rabbit Polyclonal anti-MCM2 Antibody  
Human Rectal Carcinoma stained with anti-MCM2 antibody. Rabbit IgG Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

MCM2 antibody

  MCM2 mouse monoclonal antibody, clone OTI1H2 (formerly 1H2)  
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MCM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805504) Mouse IgG2a OTI1H2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MCM2 antibody

  MCM2 mouse monoclonal antibody, clone OTI3B12 (formerly 3B12)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MCM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805520) Mouse IgG1 OTI3B12 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MCM2 antibody

  MCM2 mouse monoclonal antibody, clone OTI4C9 (formerly 4C9)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MCM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805533) Mouse IgG1 OTI4C9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MCM2 antibody

  MCM2 mouse monoclonal antibody, clone OTI3B6 (formerly 3B6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCM2 (RC202506, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCM2. Mouse IgG1 OTI3B6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

MCM2 antibody

  MCM2 mouse monoclonal antibody, clone OTI1C7 (formerly 1C7)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MCM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805621) Mouse IgG1 OTI1C7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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