MCM3 antibody

Principal name

MCM3 antibody

Alternative names for MCM3 antibody

RLF subunit beta, P102 protein, P1-MCM3, mini-chromosome maintenance protein 3, DNA replication licensing factor MCM3, DNA polymerase alpha holoenzyme-associated protein P1

SwissProt ID

A4FUD9 (Bovin), P24279 (Yeast), P25205 (Human), P25206 (Mouse), Q5ZMN2 (Chick)

Gene ID

4172 (MCM3), 17215 (Mcm3)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Chk (Chicken), Rt (Rat), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ye (Yeast), Por (Porcine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP)

Background of MCM3 antibody

MCM3 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Shukla,A., (2005) Biochem. Biophys. Res. Commun. 329 (4), 1240-1245

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MCM3 antibody

  Rabbit Polyclonal anti-MCM3 Antibody  
Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-MCM3 antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system. Rabbit IgG Aff - Purified Hu, Ms WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

MCM3 antibody

  MCM3 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using MCM3 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCM3 antibody

  Rabbit Polyclonal antibody to MCM3 (minichromosome maintenance complex component 3)  
Sample (30 ug of whole cell lysate). A: Hep G2 . 7.5% SDS PAGE. TA308761 diluted at 1:10000. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MCM3 antibody

  Rabbit polyclonal MCM3 (Thr722) antibody(Phospho-specific)  
MCM3 Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MCM3 antibody

  Anti-MCM3 Rabbit Polyclonal Antibody  
Predicted band size: 91 kDa . Positive control: K562, Jurkat, hela and 293T cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: K562 cell lysate Lane2: Jurkat cell lysate Lane3: Hela cell lysate Lane4: 293T cell lysate Lysates: 40 ug per lane Primary antibody: 1/550 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MCM3 antibody

  Anti-MCM3 Rabbit Polyclonal Antibody  
Predicted band size: 91 kDa . Positive control: K562, Jurkat and hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: K562 cell lysate Lane2: Jurkat cell lysate Lane3:Hela cell lysate Lysates: 40 ug per lane Primary antibody: 1/550 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MCM3 antibody

  Rabbit Polyclonal Anti-MCM3 Antibody  
WB Suggested Anti-MCM3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 721_B cell lysate MCM3 is supported by BioGPS gene expression data to be expressed in 721_B Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ye P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

MCM3 antibody

  Rabbit Polyclonal Anti-MCM3 Antibody  
WB Suggested Anti-MCM3 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate MCM3 is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

MCM3 (full length) antibody

Western blot analysis of MCM3 expression in Jurkat (1), Raji (2), HeLa (3), MRC-5 (4), ZR-75-1 (5), Hep-II (6), HepG2 (7), A431 (8), NIH/3T3 (9) and WR19L (10) using AM26592AF-N. Mouse IgG2a 3A2 Azide Free Hu ICC/IF, IP, P, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

MCM3 - human gene knockout kit via CRISPR

MCM3 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MCM3

  Recombinant protein of human minichromosome maintenance complex component 3 (MCM3)  
MCM3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Lysates

Catalog No.    

MCM3 overexpression lysate

MCM3 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, MCM3 (mGFP-tagged) - Human minichromosome maintenance complex component 3 (MCM3), 200 uL, >10^7 TU/mL

Lenti ORF particles, MCM3 (mGFP-tagged) - Human minichromosome maintenance complex component 3 (MCM3), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, MCM3 (Myc-DDK tagged) - Human minichromosome maintenance complex component 3 (MCM3) , 200 uL, >10^7 TU/mL

Lenti ORF particles, MCM3 (Myc-DDK tagged) - Human minichromosome maintenance complex component 3 (MCM3) , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Mcm3 (GFP-tagged) - Mouse minichromosome maintenance deficient 3 (S. cerevisiae) (Mcm3), 200 uL, >10^7 TU/mL

Lenti ORF particles, Mcm3 (GFP-tagged) - Mouse minichromosome maintenance deficient 3 (S. cerevisiae) (Mcm3), 200 uL, >10^7 TU/mL  
200 µl / €1,540.00
  OriGene Technologies, Inc.

Lenti ORF particles, Mcm3 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 3 (S, 200 uL, >10^7 TU/mL

Lenti ORF particles, Mcm3 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 3 (S, 200 uL, >10^7 TU/mL  
200 µl / €1,250.00
  OriGene Technologies, Inc.

Lenti ORF particles, Mcm3 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 3 (S. cerevisiae) (Mcm3), 200 uL, >10^7 TU/mL

Lenti ORF particles, Mcm3 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 3 (S. cerevisiae) (Mcm3), 200 uL, >10^7 TU/mL  
200 µl / €1,380.00
  OriGene Technologies, Inc.

17 Item(s)

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