MCM3 antibody

Principal name

MCM3 antibody

Alternative names for MCM3 antibody

RLF subunit beta, P102 protein, P1-MCM3, mini-chromosome maintenance protein 3, DNA replication licensing factor MCM3, DNA polymerase alpha holoenzyme-associated protein P1

SwissProt ID

A4FUD9 (Bovin), P24279 (Yeast), P25205 (Human), P25206 (Mouse), Q5ZMN2 (Chick)

Gene ID

4172 (MCM3)

Available reactivities

Hu (Human), Xen (Xenopus), Ms (Mouse), Bov (Bovine), Chk (Chicken), Rt (Rat), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ye (Yeast), Por (Porcine)

Available hosts

Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), PLA/Proximity ligation assay (PLA), Immunoprecipitation (IP), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of MCM3 antibody

MCM3 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Shukla,A., (2005) Biochem. Biophys. Res. Commun. 329 (4), 1240-1245

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MCM3 antibody

Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-MCM3 antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system. Rabbit IgG Aff - Purified Hu, Ms WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

MCM3 antibody

Western blot analysis of extracts of various cell lines, using MCM3 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCM3 antibody

Immunofluorescence of monoclonal antibody to MCM3 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2a 4F7 Purified Hu, Ms E, ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

MCM3 antibody

MCM3 monoclonal antibody (M01), clone 4F7. Western Blot analysis of MCM3 expression in K-562 ( Cat # L009V1 ). Mouse IgG2a 4F7 Ascites Hu, Ms E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

MCM3 antibody

Immunofluorescence of monoclonal antibody to MCM3 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2b 3E1 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MCM3 antibody

MCM3 monoclonal antibody (M04), clone 2H3. Western Blot analysis of MCM3 expression in human ovary cancer. Mouse IgG2a 2H3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MCM3 antibody

Sample (30 ug of whole cell lysate). A: Hep G2 . 7.5% SDS PAGE. TA308761 diluted at 1:10000. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MCM3 antibody

Predicted band size: 91 kDa . Positive control: K562, Jurkat, hela and 293T cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: K562 cell lysate Lane2: Jurkat cell lysate Lane3: Hela cell lysate Lane4: 293T cell lysate Lysates: 40 ug per lane Primary antibody: 1/550 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MCM3 antibody

Predicted band size: 91 kDa . Positive control: K562, Jurkat and hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: K562 cell lysate Lane2: Jurkat cell lysate Lane3:Hela cell lysate Lysates: 40 ug per lane Primary antibody: 1/550 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MCM3 antibody

WB Suggested Anti-MCM3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 721_B cell lysate MCM3 is supported by BioGPS gene expression data to be expressed in 721_B Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ye P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

MCM3 antibody

WB Suggested Anti-MCM3 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate MCM3 is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

MCM3 (full length) antibody

Western blot analysis of MCM3 expression in Jurkat (1), Raji (2), HeLa (3), MRC-5 (4), ZR-75-1 (5), Hep-II (6), HepG2 (7), A431 (8), NIH/3T3 (9) and WR19L (10) using AM26592AF-N. Mouse IgG2a 3A2 Azide Free Hu ICC/IF, IP, P, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

MCM3 antibody

MCM3 polyclonal antibody (A01), Lot # 050919JC01 Western Blot analysis of MCM3 expression in HepG2 ( Cat # L019V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

MCM3 antibody

MCM3 monoclonal antibody (M02), clone 2A11 Western Blot analysis of MCM3 expression in Hela NE ( Cat # L013V3 ). Mouse IgG2a 2A11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MCM3 antibody

MCM3 monoclonal antibody (M06), clone 3E11. Western Blot analysis of MCM3 expression in human spleen. Mouse IgG2a 3E11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

MCM3 antibody

Human 721_B; WB Suggested Anti-MCM3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysate. MCM3 is supported by BioGPS gene expression data to be expressed in 721_B.; MCM3 antibody - C-terminal region (ARP36644_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

MCM3 antibody

Human Lung; MCM3 antibody - C-terminal region (ARP36645_P050) in Human Lung cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

MCM3 antibody

MCM3 Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €290.00
  Boster Immunoleader

MCM3 antibody

Western Blot: MCM3 Antibody [NB100-245] - Detection of Human MCM3 by Western Blot.  Sample: Whole cell lysate (60 mcg) from HeLa cells.  Antibody: Affinity purified goat anti-MCM3 used at 0.25 mcg/ml.  Detection: Chemiluminescence. Rabbit Aff - Purified Hu WB
0.1 mg / €440.00
  Novus Biologicals Inc.

MCM3 antibody

Western Blot: MCM3 Antibody [NB100-249] - Western Blot analysis of Human MCM3, using NB100-249. Sample: Whole cell lysate (60 ug) from HeLa cells. Rabbit Aff - Purified Hu PLA, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

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