MCM4 antibody

Principal name

MCM4 antibody

Alternative names for MCM4 antibody

CDC21, CDC21 homolog, P1-CDC21, DNA replication licensing factor MCM4, minichromosome maintenance complex component 4

SwissProt ID

P33991 (Human), P49717 (Mouse)

Gene ID

4173 (MCM4), 17217 (Mcm4)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Chk (Chicken), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Goat, Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), EPair for Elisa (EPair), Immunoprecipitation (IP)

Background of MCM4 antibody

The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the postreplicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin in vitro. Cleavage of MCM3, which can be prevented by caspase inhibitors, results in the inactivation of the MCM complex (composed of at least MCM proteins 2-6) during apoptosis. A complex composed of MCM4, MCM6 and MCM7 has been shown to be involved in DNA helicase activity, and MCM5 is involved in IFN-γ-induced Stat1α transcription activation.

General readings

Ishimi,Y., et al., (2003) J. Biol. Chem. 278 (27), 24644-24650

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MCM4 antibody

  Anti-MCM4 Rabbit Polyclonal Antibody  
Predicted band size: 97 kDa . Positive control: NIH/3T3, Hela and K562 cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: NIH/3T3 cell lysate Lane2: Hela cell lysate Lane3: K562 cell lysate Lysates: 50 ug per lane Primary antibody: 1/700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MCM4 antibody

  Rabbit Polyclonal Anti-MCM4 Antibody  
WB Suggested Anti-MCM4 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:12500; Positive Control: Jurkat cell lysate MCM4 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCM4 antibody

  Rabbit Polyclonal Anti-MCM4 Antibody  
WB Suggested Anti-MCM4 Antibody Titration: 2.5 ug/ml; ELISA Titer: 1:62500; Positive Control: Jurkat cell lysate MCM4 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCM4 antibody

  Rabbit Polyclonal Anti-MCM4 Antibody  
WB Suggested Anti-MCM4 Antibody Titration: 5.0 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate MCM4 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCM4 antibody

Immunohistochemistry (IHC) analyzes of MCM4 antibody (Cat.-No.: AP01449PU-N) in paraffin-embedded human testis tissue. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

MCM4 antibody

Detection of human MCM4 in whole cell lysates of HeLa cells by Western blotting using Goat anti Human MCM4 (SP2048P) Goat Aff - Purified Hu WB
0.1 mg / €450.00
  Acris Antibodies GmbH

MCM4 pSer54 antibody

Western blot analysis of extracts from COS7 cells treated with nocodazole 1ug/ml 16h, using MCM4 (Phospho-Ser54) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €230.00
  Assay Biotech

MCM4 pSer54 antibody

Western blot analysis of extracts from COS7 cells treated with nocodazole 1ug/ml 16h, using MCM4 (Phospho-Ser54) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €330.00
  Assay Biotech

MCM4 antibody

Immunohistochemistry analysis of paraffin-embedded human testis tissue, using MCM4 (Ab-54) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
50 µg / €230.00
  Assay Biotech

MCM4 antibody

Immunohistochemistry analysis of paraffin-embedded human testis tissue, using MCM4 (Ab-54) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

MCM4 - human gene knockout kit via CRISPR

MCM4 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

MCM4 - human gene knockout kit via CRISPR

MCM4 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

MCM4 - human gene knockout kit via CRISPR

MCM4 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Mcm4 - mouse gene knockout kit via CRISPR

Mcm4 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Mcm4 - mouse gene knockout kit via CRISPR

Mcm4 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Mcm4 - mouse gene knockout kit via CRISPR

Mcm4 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Mcm4 - mouse gene knockout kit via CRISPR

Mcm4 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

MCM4 (transcript variant 2)

  Recombinant protein of human minichromosome maintenance complex component 4 (MCM4), transcript variant 2  
MCM4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

MCM4 (transcript variant 1)

  Recombinant protein of human minichromosome maintenance complex component 4 (MCM4), transcript variant 1  
MCM4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, MCM4 (mGFP-tagged) - Human minichromosome maintenance complex component 4 (MCM4), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, MCM4 (mGFP-tagged) - Human minichromosome maintenance complex component 4 (MCM4), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

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