MCM4 antibody

Principal name

MCM4 antibody

Alternative names for MCM4 antibody

CDC21, CDC21 homolog, P1-CDC21, DNA replication licensing factor MCM4, minichromosome maintenance complex component 4

SwissProt ID

P33991 (Human), P49717 (Mouse)

Gene ID

4173 (MCM4), 17217 (Mcm4)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Chk (Chicken), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Goat, Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), EPair for Elisa (EPair), Immunoprecipitation (IP)

Background of MCM4 antibody

The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the postreplicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin in vitro. Cleavage of MCM3, which can be prevented by caspase inhibitors, results in the inactivation of the MCM complex (composed of at least MCM proteins 2-6) during apoptosis. A complex composed of MCM4, MCM6 and MCM7 has been shown to be involved in DNA helicase activity, and MCM5 is involved in IFN-γ-induced Stat1α transcription activation.

General readings

Ishimi,Y., et al., (2003) J. Biol. Chem. 278 (27), 24644-24650

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

MCM4 antibody

Immunoperoxidase of purified MaxPab antibody to MCM4 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml] Mouse Serum Hu E(detection), ICC/IF, P, WB
50 µl / €370.00
  Abnova Taiwan Corp.

MCM4 antibody

Immunoperoxidase of purified MaxPab antibody to MCM4 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml] Mouse Purified Hu E(detection), ICC/IF, P, WB
50 µg / €370.00
  Abnova Taiwan Corp.

MCM4 antibody

MCM4 MaxPab rabbit polyclonal antibody. Western Blot analysis of MCM4 expression in mouse testis. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

MCM4 antibody

Predicted band size: 97 kDa . Positive control: NIH/3T3, Hela and K562 cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: NIH/3T3 cell lysate Lane2: Hela cell lysate Lane3: K562 cell lysate Lysates: 50 ug per lane Primary antibody: 1/700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

MCM4 antibody

WB Suggested Anti-MCM4 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:12500; Positive Control: Jurkat cell lysate MCM4 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCM4 antibody

WB Suggested Anti-MCM4 Antibody Titration: 2.5 ug/ml; ELISA Titer: 1:62500; Positive Control: Jurkat cell lysate MCM4 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCM4 antibody

WB Suggested Anti-MCM4 Antibody Titration: 5.0 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate MCM4 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

MCM4 antibody

Immunohistochemistry (IHC) analyzes of MCM4 antibody (Cat.-No.: AP01449PU-N) in paraffin-embedded human testis tissue. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

MCM4 antibody

Detection of human MCM4 in whole cell lysates of HeLa cells by Western blotting using Goat anti Human MCM4 (SP2048P) Goat Aff - Purified Hu WB
0.1 mg / €450.00
  Acris Antibodies GmbH

MCM4 antibody

MCM4 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

MCM4 antibody

MCM4 Rabbit Serum Hu, Ms E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

MCM4 antibody

MCM4 Mouse IgG2a 1A6 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

MCM4 pSer54 antibody

Western blot analysis of extracts from COS7 cells treated with nocodazole 1ug/ml 16h, using MCM4 (Phospho-Ser54) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €205.00
  Assay Biotech

MCM4 pSer54 antibody

Western blot analysis of extracts from COS7 cells treated with nocodazole 1ug/ml 16h, using MCM4 (Phospho-Ser54) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €310.00
  Assay Biotech

MCM4 antibody

Immunohistochemistry analysis of paraffin-embedded human testis tissue, using MCM4 (Ab-54) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
50 µg / €205.00
  Assay Biotech

MCM4 antibody

Immunohistochemistry analysis of paraffin-embedded human testis tissue, using MCM4 (Ab-54) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / €310.00
  Assay Biotech

MCM4 antibody

Human Jurkat; WB Suggested Anti-MCM4 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:12500. Positive Control: Jurkat cell lysate. MCM4 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; MCM4 antibody - middle region (ARP36649_T100) in Human Jurkat cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

MCM4 antibody

Human Jurkat; WB Suggested Anti-MCM4 Antibody Titration: 2.5ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate. MCM4 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; MCM4 antibody - N-terminal region (ARP36662_T100) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms WB
0.1 mg / €310.00
  AVIVA Systems Biology

MCM4 antibody

Human Jurkat; WB Suggested Anti-MCM4 Antibody Titration: 5.0ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate. MCM4 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; MCM4 antibody - C-terminal region (ARP36663_T100) in Human Jurkat cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

MCM4 antibody

MCM4 Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

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